AIM: To establish the HPLC fingerprints of semen Trigonellae processed with salt-water.(METHODS:)HPLC analysis was performed on a phenomenex Hydro-RP 80A(4.6 mm?250 mm,5 ?m) with mobile phase acetonitrile-water under isocratic and gradient elutions,and detection wavelength was 211nm,the flow rate was 1 mL/min,column temperature was 40 ℃. RESULTS: HPLC fingerprint of 17 main peaks was separated.It was found that the preparative HPLC of standardized processing saline semen Trigonellae from different places have no significant differences. CONCLUSION: The method is reliable,accurate and can be used for quality control of saline semen Trigonellae.

Panax notoginseng (BurK.) F.H.Chen is a traditional Chinese medicinal material with a time-honored history of cultivation.There are a series of problems,such as high pesticide residues,serious disease and pest,and continuous cropping obstacles in the process of the cultivation of notoginseng.Pollution-free cultivation is an effective strategy for the sustainable development of notoginseng industry.We herein summarized three points of the pollution-free cultivation of notoginseng in this review.The standard of lands suitable for the cultivation of notoginseng was established on the basis of the analysis of medicinal plants around global producing areas.The integrated measures of soil improvement were put forward by cfficient rotation and soil disinfection with new varieties breeding combined with the management of water,light and fertilization,and the safe and low-toxic methods of disease and pest control.Additionally,the mode of wild tending should be carried out when the marker-assisted breeding of new varieties was developed,and the platform of comprehensive disease and pest control was founded.Above-mentioned points can effectively perfect and optimize the pollution-free cultivation of notoginseng and promote sustainable development of notoginseng industry.

Objective To investigate the effects and the mechanism of thrombospondin 4 (Thbs4) gene-edited bone marrow mesenchymal stem cells (BMSCs) transplantation on vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) in diabetic rats with hind limb ischemia. Methods Thirty Sprague-Dawley rats were randomly divided into the model group, BMSCs treatment group and Thbs4-BMSCs treatment group on average. After constructing the typeⅡdiabetic rat model with hind limb ischemia, 100μl normal saline, BMSCs suspension and Thbs4-BMSCs suspension (cell number: 2×106) were locally injected into the ischemic injury area of rats for the model group, BMSCs group and Thbs4-BMSCs group, respectively. The rats were sacrificed on the 14th day after stem cell transplantation, and the muscle tissues near the ischemic area were collected. The relative expression of VEGF and p-Smad2/3 protein was detected by Western Blot. The Ang-1 protein expression was detected by immunofluorescence staining. The levels of related genes were detected by qRT-PCR, and the von Willebrand Factor (vWF) protein expression was detected by immunohistochemistry staining. Results The relative expression levels of VEGF, Ang-1 and vWF protein in the Thbs4-BMSCs group were significantly higher than those in the model group and BMSCs group (VEGF protein:P<0.01 and P<0.05). The mRNA expression of VEGF and Ang-1 were significantly up-regulated, the differences were statistically significant(VEGF mRNA:all P<0.01;Ang-1:P<0.01 and P<0.05). The expression of p-Smad2/3 protein in the Thbs4-BMSCs group was significantly higher than that in the model group and the BMSCs treatment group (all P<0.01). The expression of p-Smad2/3 protein was significantly decreased after the addition of p-Smad2/3 pathway inhibitor, the differences were statistically significant (P<0.05). Conclusions Thbs4-BMSCs transplantation can effectively promote angiogenesis in diabetic rats with hind limb ischemia, and the effect of angiogenesis may be related to the activation of Smad2/3 signaling pathway.

Objective To investigate the prevalence of complication and related risk factors in Uygur patients with type 2 diabetes mellitus (T2DM).Methods Total 1 507 Uygur patients aged above 20 with T2DM were recruited in the First Affiliated Hospital of Xinjiang Medical University from January 2010 to October 2015.Clinical characteristics,biochemical parameters and related complications were analyzed in different age groups of patients.Multivariate logistic regression was performed to determine associated factors for diabetic complications.Results Among 1 507 cases,there were 61.3％ male patients (n=924),37.4％ (n=564) with diabetes duration ≥5 years,12.5％ (n=189) with HbA1c＞10％,31.5％ (n=475) with hypertension,28.8％ (n=434) with smoking history and 37.4％ (n=564) patients without antidiabetic drug treatment.The average body mass index (BMI) of patients was (27.4±5.2)kg/m2 with a obesity rate of 43.0％ (648/1 507),and 65.0％ (980/1 507) had physical exercise＜150 min/week.The prevalence of diabetic complication was 70.6％(1 064/1 507),in which 28.7％,19.2％ and 22.7％ cases had 1,2 and 3 or more complications,respectively.The proportion of patients with coronary heart disease,stroke/transient ischemic attack and large artery atherosclerosis was 32.4％,10.4％ and 35.8％,respectively.The proportion of patients with diabetic retinopathy,nephropathy and neuropathy were 23.4％,6.5％ and 35.8％,respectively.Patients aged ≥60 years had highest prevalence of large artery atherosclerosis (38.2％,163/427) and diabetic retinopathy (27.2％,116/427);patients in age group 50-59 years had highest prevalence of diabetic neuropathy (40.3％,207/514).Multivariate logistic regression analysis showed that HbA1c＞10％ (OR=2.74,95％CI:1.40-5.34),obesity (OR=1.54,95％CI:1.07-2.21),systolic pressure≥140 mmHg (1 mmHg=0.133 kPa) (OR=1.42,95％CI:1.01-1.99),diastolic pressure≥90 mmHg (OR=0.37,95％CI:0.21-0.67),smoking (OR=1.53,95％CI:1.09-2.15) and physical aetivity＜150 min/week (OR=1.42,95％CI:1.07-1.89) were the risk factors of diabetic complications.Conclusion Uygur patients with type 2 diabetes in Xinjiang show a relatively high prevalence of complications.Poor glycemic control,obesity,hypertension,smoking and physical inactivity may contribute to development of complications,while well-controlled blood glucose and hypertension and quitting smoking and increasing physical activity may reduce diabetic complications.