Objective To establish transgenic mouse models expressing human HSP22 protein.Methods pCAGGS-HA-Wt HSP22 transgenic expressing vector carrying human HSP22 gene was constructed by gene recombination technology.The linearized DNA was got by SalI、Hind Ⅲ and BsaⅪ digestion of PCAGGS-HA-Wt HSP22,purified and microinjected into fertilized eggs from C57BL mice.The tail DNA of pups was tested by PCR and DNA sequencing.Expression of human HSP22 protein was detected by western blot with anti-HA tag monoclonal antibody.Results 4 transgenic founder mice (Tg646,Tg648,Tg649,Tg661) carrying human HSP22 gene were identified by PCR and DNA sequencing.The human HSP22 protein was expressed in the lines Tg646,Tg648 and Tg649 founder mice,but was not expressed in the line Tg661 founder mouse.Conclusions The mouse models expressing human HSP22 protein are established successfully and provide the foundation for HSP22 gene research in vivo.

Objective? To explore the effects of WeChat combined with module teaching in minimally invasive surgery nursing training. Methods? From July 2016 to June 2018, 56 junior nurses in operating room were randomly divided into control group and experimental group, 28 nurses in each group by random table. Control group carried out routine teaching combined with module teaching, while experimental group adopted WeChat combined with module teaching. We compared the theoretical achievements, skills performance and satisfaction of surgeons with nursing. Results? Twelve months after training, the theoretical achievements and skills performance of experimental group were (76.93±5.31) and (78.79±4.86) respectively, higher than those of control group with statistical differences (t=-3.34, -3.13;P＜ 0.05). Satisfaction of surgeons with nursing provided by nurses was (94.68±3.37) in experimental group and (72.26±4.67) in control group with a statistical difference (t=-35.64,P＜ 0.05). Conclusions? The application of WeChat combined with module teaching in minimally invasive surgery nursing training for junior nurses can stimulate the enthusiasm of autonomous learning, raise the theoretical achievements and skills performance and improve satisfaction of surgeons with nursing for surgeries.