Objective To compare the effects between bloodletting at auricular points and acupuncture at specific points [Yintang (GV 29) or Yanglao (SI 6)] for analgesia in acute lumbar sprain.Method Totally 100 patients with acute lumbar sprain were randomized into a treatment group and a control group, 50 cases in each group. The treatment group was intervened by bloodletting at auricular points, while the control group was by acupuncture at specific points [Yintang (GV 29) or Yanglao (SI 6). The Present Pain Intensity (PPI) from the Short-form McGill Pain Questionnaire was taken as the observation index. The changes of PPI were compared respectively 10 min and 30 min after treatment.Result The PPI scores were significantly changed 30 min after treatment in both groups (P<0.05). The PPI scores in the treatment group were significantly different from that in the control group both 10 min and 30 min after intervention (P<0.05).Conclusion Bloodletting at auricular points and acupuncture at specific points are both effective in releasing pain in acute lumbar sprain, while bloodletting at auricular points can produce a more significant real-time analgesic effect.

Purpose To find the reasons and explore the appropriate countermeasures for failure ureteroscopy operation.Methods The clinical data of 74 cases with failure ureteroscopy operation for ureteral calculi were analysed respectively.Results The failure reasons including the difficult of ureteroscopy insert (46 lateral),difficult of calculi disposal (12 lateral) and ureteral injure (15 lateral).ESWL(34 sides) and open surgery(44 sides) were remedial measures after failure ureteroscopy and the success rates were 85 3%, 100% respectively.nephrectomy were carried out in and 1 case died from serious uriaemia.The mortality was only 1.4%.Conclusions The reasons of failure including unable approach the stone, difficult to disposal calculi and ureteral injury. The effect is related to applicances, experience, indications . ESWL and open surgery are the suitable remedy measures according to the failure reason.

Microtubules play important roles in mitotic spindle assembly and chromosome segregation to maintain normal cell cycle progression. A number of microtubule-associated proteins have been identified in epithelial and neural cell cultures; however, their physiological significance is not well characterized due to the lack of appropriate in vivo animal models. Nucleolar spindle-associated protein (NuSAP) is a microtubule-binding protein and is reported to be involved in mitosis by cell culture studies. In this report, we identified the zebrafish homologue of human NuSAP and investigated its expression profile and functions. Using in situ hybridization, we demonstrated that transcripts of zebrafish nusap1 are specifically expressed in the retina, forebrain, hindbrain and neural crest. When the in vivo expression of nusap1 was knocked down through antisense oligonucleotide morpholino technology, the morphants of nusap1 showed impaired morphogenesis in the trunk and yolk extension, implying the involvement of Nusap1 in cell migration. Mechanistic studies revealed that nusap1 morphants have an altered expression pattern of neural crest markers crestin and sox9b, but normal expression of blood vessel and notochord markers gata1 and shh. In addition, nusap1 mRNA injection caused serious apoptosis in retina and hindbrain tissue, and these phenotypes can be rescued by co-injection of morpholino against nusap1. These observations not only suggest a role for Nusap1 in connecting apoptosis with cell migration, but also provide strong evidences that Nusap1 is potentially involved in morphogenesis in vertebrates.
Amino Acid Sequence ; Animals ; Animals, Genetically Modified ; Apoptosis ; genetics ; physiology ; Base Sequence ; Cell Movement ; genetics ; physiology ; Cloning, Molecular ; DNA Primers ; genetics ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Knockdown Techniques ; Humans ; In Situ Hybridization ; Microtubule-Associated Proteins ; genetics ; physiology ; Molecular Sequence Data ; Neural Crest ; cytology ; embryology ; physiology ; Phylogeny ; Sequence Homology, Amino Acid ; Zebrafish ; embryology ; genetics ; Zebrafish Proteins ; genetics ; physiology