4.Histological observation on rabbit skull osteoinduction by advanced platelet-rich fibrin
Zhili JIAO ; Xiaoling XIE ; Dongmei FU ; Feng ZHAO ; Yong SUN
Chinese Journal of Tissue Engineering Research 2017;21(14):2208-2214
BACKGROUND: Previous studies have proved platelet-rich fibrin (PRF) with osteoinduction ability, and the centrifugal speed and time to prepare rabbit advanced PRF (A-PRF) with the most similar structure to that of human PRF have been determined.OBJECTIVE: To observe the histological changes during A-PRF-induced osteogenesis.METHODS: Thirty Japanese white rabbits were randomly divided into A-PRF and blank control groups (n=15 per group).The full-thickness defect models were established on the rabbit parietal bone, followed by implanted with A-PRF or nothing, respectively. The model rabbits were killed immediately, at 2, 4, 8 and 12 weeks after modeling, to grossly observe the bone formation, and the histological changes in the defect region were observed through hematoxylin-eosin staining, Masson staining and immunohistochemistry.RESULTS AND CONCLUSION: Unhealed defects were observed in the blank control group. Gross and histological observations showed that the speed, amount and maturity of bone formation in the A-PRF group were significantly better than those in the blank control group immediately, 4, 8 and 12 weeks after modeling (P < 0.05). Our findings suggest that the rabbit skull bone defect is successfully established. A-PRF can induce osteogenesis, and more mature newly born bones appear with time. Additionally, osteoclasts can act with osteoblasts synergically under the A-PRF induction to promote the bone formation.
6.Changes of Somatostatin Levels in Plasma and Cerbrospinal Fluid of Children with Convulsive Diseases
xiang-yang, GUO ; fu-yong, JIAO ; xin-li, ZHANG ; hong-tao, LEI ; qing, NIU
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To study the changes of somatostatin(SOM) in plasma and cerebrospinal fluid (CSF) of children with convulsive diseases.Methods Sixty-seven children with convulsive diseases were studied as following:obtaining the samples of plasma in the 1st and 7th day after being in hospital,and the samples of CSF in the 1st after being in hospital.We investigated the changes of SOM in plasma and CSF with radioimmunoassay(RIA).Results 1.Convulsive group:the concentration of SOM in plasma in the 7th day(29.47?9.40 ng/L) was significant lower than that in the 1st day(39.23?11.00 ng/L)(t=21.530 P0.05).The concentration of SOM in plasma in the 1st day in control group was(19.58?6.04) ng/L.There were significant differences in convulsive group and encephalitis group without convulsion, control group(t= 6.847,7.921 P
7.Effect of childhood physical and emotional abuse on psychological health in undergraduate students.
Qin-Qin YAN ; Wen-Yan JIAO ; Jin LIN ; Fu-Yong JIAO
Chinese Journal of Contemporary Pediatrics 2009;11(8):675-678
OBJECTIVETo study the effect of childhood physical and emotional abuse on psychological health in undergraduate students.
METHODSA questionnaire investigation (Bernstein, revised) on childhood abuse history was performed in 1 200 undergraduate students in Xi'an. Their psychological states were assessed by Symptom Check List-90.
RESULTSSix hundred and sixty-eight students (55.7%) had physical and emotional abuse experiences, including humiliation, corporal punishment, beating or activity confinement, before 16 years old. Twenty-one percent of the 1 200 students experienced beating, even serious beating with equipment. The students who experienced serious physical and emotional abuse during childhood were more prone to the development of somatization symptoms, obsession, interpersonal sensitivity, depression, anxiety, hostility, phobic anxiety and paranoia than those students without abuse experiences.
CONCLUSIONSChildhood physical and emotional abuse is common in China and this may adversely affect the psychological health of undergraduate students who have experienced it.
Adolescent ; Adult ; Child Abuse ; psychology ; statistics & numerical data ; Female ; Humans ; Incidence ; Male ; Mental Health ; Retrospective Studies ; Students ; psychology ; Universities
8.Vitamin D receptor gene polymorphism in patients with rickets.
Guang WANG ; Li-wei SUN ; Li-xin JIAO ; Qi CHEN ; Yu LIU ; Yu-qi LIU ; Cheng-xun WANG ; Yong-ping WANG ; Yong-fu LI
Chinese Journal of Pediatrics 2004;42(10):788-789
9.Safety and efficacy of ruxolitinib as a salvage regimen for patients with refractory acute and chronic graft-versus-host disease after allogeneic hematopoietic stem cell transplantation.
Hao AI ; Yue Wen FU ; Yong Qi WANG ; Xue Li JIAO ; Xu Dong WEI ; Yong Ping SONG
Chinese Journal of Hematology 2018;39(12):1026-1029
10.Efficacy comparison of lumber disc herniation treated with mild moxibustion at Yaoyangguan (GV 3) under different conditions.
Xiu-jun XIE ; Ri-xin CHEN ; Yong FU ; Lin JIAO ; Bo ZHANG ; Jun XIONG ; Lv HE ; Hai-liang QIANI ; Jun-xian LI ; Xiao-jun LUO
Chinese Acupuncture & Moxibustion 2014;34(11):1077-1080
OBJECTIVETo compare the difference in clinical efficacy on lumber disc herniation (LDH) treated with Yaoyangguan (GV 3) between mild moxibustion under thermosensitive condition and that under non-thermo-sensitive condition.
METHODSFifty-seven LDH patients were selected as the study objects. Mild moxibustion at Yaoyangguan (GV 3) was applied for 45 min each time. Additionally, the conventional acupuncture was given, once a day, for 20 days. At the end of treatment, two groups were classified. A thermosensitive acupoint group (thermosensitive group) was composed of the cases with thermo-sensitization at Yaoyangguan (GV 3) and presenting for > or =4 times in the entire treatment. A non-thermosensitive acupoint group (tranquilization group) was composed of the cases without thermo-sensitization or the frequency of thermo-sensitization <4 times in the entire treatment. The modified Japanese orthopedics association scoring system (M-JOA) was adopted to observe the cases before and after treatment and 6 months after treatment in the two groups. The efficacy was compared between the two groups.
RESULTSAfter treatment and in 6 months after treatment, the score of M-JOA was apparently reduced as compared with that before treatment in the two groups (all P<0.05). The score reducing in the thermosensitization group was more obvious than that in the tranquilization group (both P<0.01). After treatment and in 6 months after treatment, the curative rate and remarkably effective rate were 89.7% (26/29) and 79.3% (23/29), which was better than 71.4% (20/28) and 60.7% (17/28, both P<0.05) in the tranquilization group separately.
CONCLUSIONMild moxibustion at acupoint under thermosensitive condition achieves the better short-term and long-term effects as compared with that under non-thermosensitive condition.
Acupuncture Points ; Adult ; Female ; Humans ; Intervertebral Disc Displacement ; therapy ; Male ; Middle Aged ; Moxibustion ; Treatment Outcome