Animals ; Cytidine ; analogs & derivatives ; pharmacology ; DNA Methylation ; drug effects ; DNA Modification Methylases ; metabolism ; Gene Knockout Techniques ; Histone Acetyltransferases ; metabolism ; Histone Deacetylase Inhibitors ; pharmacology ; Humans ; Hydroxamic Acids ; pharmacology ; Neoplasms ; genetics ; physiopathology ; therapy ; RNA Interference ; Valproic Acid ; pharmacology

Objective To explore the contribution of endoplasmic reticulum stress to cardiac myocyte apoptosis in mouse congestive heart failure induced by Doxorubicin.Methods Doxorubicin (DOX)was administered intraperitoneally to rats,2 mg/kg doses over 8 weeks.Echocardiographic and hemodynamics measurements were obtained at 8 weeks after treatment.Then left ventricular (LV) samples were collected.The successfully established rats were divided into 3 groups (Fu Shen Ke Li 40 mg/kg,60 mg/kg,and 80 mg/kg).After 5 weeks post the operation,an echocardiographic was performed on all the rats under narcotism,Western blot to test the B-cell lymphoma-2 (Bcl-2),myeloid cell leukemia-1 (Mcl-1),Bcl-2-Associated X (Bax),Caspase-3,C/EBP homologous protein (CHOP),and 78-kDa glucose-regulated protein(GRP78),and reverse transcription-polymerase chain reaction (RT-PCR) to test the mRNA expression levels of Mcl-1,and NOXA.Results The left ventricular end-diastolic diameter (LVEDD),left ventricular end systolic diameter (LVESD),left ventricular ejection fraction (LVEF) prompted modeling success The left ventricular end-diastolic diameter (LVEDD),left ventricular ejection fraction (LVEF),and fractional shortening (FS) have been improved in high and middle groups of Fu Shen Ke Li.Compared to the model group,higher expression levels of Bcl-2,and Mcl-1 were,lower expressions of Bax,NOXA,CHOP,and GRP78 were (P ＜ O.05) in 3 groups of Fu Shen Ke Li.Compared to the model group,there were higher mRNA expression level of Mcl-1 was,lower mRNA expression of NOXA was (P ＜ 0.05) in 3 groups of Fu Shen Ke Li.Conclusions Fu Shen Ke Li depresses endoplasmic reticulum stress,which can reduce the apoptosis of myocardial cells,and ultimately achieve the purpose of treatment of heart failure.

BACKGROUND: Vascular dementia is a syndrome characterized by cognitive impairment, which is induced by ischemic or hemorrhagic apoplexy or ischemic-anoxic brain injury. It is assigned to "imbecility", "dementia", "sluggish consciousness", "susceptible to forget things" etc. By traditional Chinese medicine. Impairments of memory and cognition etc. Caused by dementia seriously affect psychosomatic health and quality of life of elderly people.OBJECTIVE: To observe the effects of shuanggen cephalocathartic decoctum on learning, memory and spatial discrimination ability of vascular dementia rats.DESIGN: Randomized control trail taking experimental animals as objects.SETTING: Teaching and Research Section of Basic Theory of Traditional Chinese Medicine, Preclinical Medicine College, Nanjing University of Traditional Chinese Medicine.MATERIALS: The experiment was performed in Laboratory of Viscerastate of Teaching and Research Section of Basic Theory of Traditional Chinese Medicine, Preclinical Medicine College, Nanjing University of Traditional Chinese Medicine, from January to February 2004. A total of 40 male SD rats were selected and randomly divided into sham operation group, model group, model plus traditional Chinese medicine group and model plus Western medicine group, with 10 in each group. Before the experiment, all the rats were adaptively fed for one week, free drinking and eating before and during the experiment. Medicine and reagent are shuanggen cephalocathartic decoctum and Nimotop tablet, and main experimental apparatuses are DTT-2 jumping apparatus and SMG-2 water maze apparatus.METHODS: Blood was collected from left ventricle under aseptic condi tion, and dried in incubator at 37 ℃, after trituration, it was sifted with sieve of 200μm meshes and embolus was prepared. 1 mg embolus and 0.3 mL physiological saline was mixed and shaken up into suspension. Common carotid artery was temporarily occluded with a vascular clamp, and external carotid artery was retrogradely intubated and the embolus suspension was injected into encephalon to establish model of multiple cerebral infarction. In sham operation group, canulate tubule was intubated only to the bifurcation of common carotid artery, with no fluid injected in. Before the experiment, rats in model group and sham operation group were normally fed, while rats in model plus traditional Chinese medicine group were additionally given shuanggen cephalocathartic decoctum [4 g/(kg·d)] infusion and rats in model plus western medicine group were additionally given Nimotop [2 mg/(kg·d)] infusion, once a day for consecutive 15 days. Passive escape jumping experiment was conducted on rats in each group on the 9th day.Recorded the reaction time for rat jumping onto the rubber cushion after electric shock and the frequency of mistakes of suffering electric shock due to jumping down from the platform in 3 minutes, and took the results as learning indexes. The test was repeated on the 10th day. Put the rat into the box for 3-minture adaptation, then put it on the rubber cushion; recorded the latency for rat first jumping down from the rubber cushion and the frequency of jumpingdown from the rubber cushion in 3 minutes, and took the results as memory indexes. Water maze spatial discrimination experiment was conducted on rats in each group on the 11th day, recorded the frequency of mistakes of rats entering dead end and the latency for landing on safe platform. Every time, took a 40-second rest after swimming. Each rat was trained 10 times a day for consecutive three days. MAIN OUTCOME MEASURES: Results of passive escape jumping experiment and water maze spatial discrimination experiment. RESULTS: Each group had a rat dead in the process of operation except sham operation group in which all rats moved normally. Totally 37 rats entered results analysis. ① Results of passive escape jumping experiment: On the 9th day, compared with model group, the reaction time of rats in sham operation group, model plus traditional Chinese medicine group and model plus western medicine group significantly shortened (P < 0.01 -0.05), the frequency of mistakes in 3 minutes remarkably decreased in sham operation group and model plus traditional Chinese medicine group (P < 0.05); on the 10th day, the latency was significantly prolonged in sham operation group as compared with model group (P < 0.05); compared with model group, the frequency of mistakes in 3 minutes significantly decreased in sham operation group, model plus traditional Chinese medicine group and model plus western medicine group (P < 0.05). ② Results of water maze spatial discrimination: On the 3rd day, compared with model group, the frequency of mistakes of rats entering dead end significantly decreased in sham operation group, model plus traditional Chinese medicine group and model plus western medicine group (P < 0.001-0.05); the frequency of mistakes in model plus traditional Chinese medicine group obvi ously decreased as compared with model plus western medicine group (P < 0.05). During the 2nd and 3rd days of water maze experiment, the time for the rat reaching terminal was significantly shortened in sham operation group, model plus traditional Chinese medicine group and model plus western medicine group as compared with model group (P < 0.05).CONCLUSION: Shuanggen cephalocathartic decoctum can significantly improve learning and memory ability of rat model of vascular dementia, and is more effective than Nimotop in the aspect of improving spatial discrimination ability of rat.

AIM: To analyze the postoperative infection of pathological myopia with pocket scleral reinforcement. ·METHODS:The clinical data of 167 cases of pathological myopia treated with pocket scleral reinforcement in June to December 2014 were analyzed. The postoperative infection rate, the resistance of pathogenic bacteria were analyzed, and the related factors of infection were analyzed. ·RESULTS: A total of 286 eyes were obtained in 167 patients. The infection rate was 6. 3% in 10 patients ( 18 eyes) . There were 30 pathogenic bacteria isolated from the 18 infected eyes, in which were 10 Staphylococcus aureus, 10 Staphylococcus epidermidis, 6 Klebsiella pneumoniae, 4 Pseudomonas aeruginosa infection. Gram positive bacteria showed higher resistance to penicillin, ampicillin and ciprofloxacin, and were sensitive to vancomycin. The resistance rates of gram negative bacteria to cefotaxime were higher, but to imipenem was low. The two groups of patients age, culture level, operation time, the number of operation, intraoperative nursing staff seniority, postoperative medication compliance rate was statistically significant (P<0. 05), which related to the infection after pocket scleral reinforcement. ·CONCLUSION:The infection caused by Staphylococcus aureus is the most common after pocket scleral reinforcement, and it is sensitive to vancomycin, and gram negative bacteria is sensitive to imipenem. Shortening the operation time, using the experienced nursing staff to cooperate, reducing the number of operation and improving the compliance of the patients can reduce the postoperative infection.

Objective To compare the clinical results of choledocholithotomy by laparoscopic surgery and open surgery.Methods One hundred and Sixty-nine patients with cholecystoithiasis combined with choledocholithiasis were divided into laparoscopic surgery group(85 cases of laparoscopic choledocholithotomy,LCD-TD) and open surgery group(84 cases of open choledocholithotomy,OCH-TD).The clinical data in the two groups were compared and analyzed.Results The amount of intraoperative bleeding,the recover time of intestinal peristalsis after treatment,length of hospital stay,cases of postoperative pain and biliary fistulas in laparoscopic surgery group were evidently better than those of open surgery group(all P

Objective To understand the relationship between Toxoplasma gondii infection and pet-breeding and hygienic status. Methods One or two districts were chosen from Jining City, Za-ozhuang City and Linyi City as study areas and the questionnaire surveys were carried out for hygienic status, etc. The pet-breeders and non-pet-breeders were paired by sex, age, living place and occupation so on in a ratio of one to one. Blood samples taken from each were tested for antibodies against T. gondii with ELISA in a double-blind method. Results The total infection rate was 14. 25%, much higher than that investigated ten years ago (1. 53%). The mean positive infection rate was 19.91% in the pet-breeding groups and 8.60% in the control groups (x2 = 11.57, P

ObjectiveTo explore the morbidity rate of deviation of nasal septum about personnels who take part in physical examination in the enterprise and facilities of Qinhuangdao,and clinic symptom.MethodsTransverse questionnaire investigation and normal physical examination were adopted.2604 personnels who take part in physical examination were choiced.ResultsMorbidity rate of deviation of nasal septum was 17.6％.The rate of men' s exceeds that of women' s.ConclusionMorbidity rate of deviation of nasal septum was rather high,clinic symptom of snuffle was primary.

Objective:To explore the influence of tumor cells on CD4~+ CD25~+ Treg cells via TLRs.Methods:The numbers changes of CD4~+ CD25~+ Treg cells in the co-culture system of Lewis lung cancer cells and splenic lymphocytes were detected by flow cytometry;The expression of Foxp3 and TLR1-9 mRNA after co-culture were detected by RT-PCR;TLR9 expression on Lewis lung cancer cells was blocked by TLR9 receptor antagonist chloroquine.Results:Compared with control group,the number of CD4~+ CD25~+ Treg cells and Foxp3 mRNA expression were significantly increased in the co-culture group (P＜0.05).The expression of a variety of TLRs were affected by the co-culture of lymphocytes with Lewis lung cancer cells,and TLR9 mRNA expression was significantly increased compared with that of the control group (P＜0.05);Blocking TLR9 of Lewis lung cancer cells significantly reduce CD4~+ CD25~+ Treg cells and Foxp3 mRNA (P＜0.05).Conclusion:Lewis lung cancer cells could increase both number and function of CD4~+ CD25~+ Treg cells through inducing TLR9 expression in immunocells.This might be one of mechanisms of tumor-induced immune tolerance,and by which to contribute to the occurrence and progression of tumors.

Bronchiectasis combined with pulmonary carcinoma in situ is very rare condition.This study reported four cases and reviewed some literatures.The data of 66 cases treated from August 2012 to October 2013,whose initial diagnoses as bronchiectases were analysed.Four cases were diagnosed postoperatively as bronchiectasis with pulmonary carcinoma in situ after sugery,accounted for 6％ of surgical patients for bronchiectasis.Bronchiectasis has the risk of combined with pulmonary carcinoma in situ,this should be brought to the attention.

Objective To promote the successful treatments of massive hemoptysis by summarizing the clinical data of 14 patients receiving surgery.Methods 14 cases of massive hemoptysis were retrospectively analysed,Eight male and 6 femal,aged 27-64 (45 ± 18) years including the application of CT,bronchoscopy and extracorporeal membrane oxygenation (ECMO).Results 14 patients received preoerative bronchoscopy and emergency operation.In addition,hymoptysis had not occurred after the surgery in these cases.Conclusion The standardized clinical pathways for massive hemoptysis and the application of preoerative bronchoscopy is essential for emergency treatment of massive hemoptysis.