1.Effect of Kangfuxin on ulcerative colitis induced by trinitrobenzene sulfonic acid in rats
Han-Chao ZHANG ; Peng-Chuan WANG ; Heng LIU ; Fu-Neng GENG ; Xiu-Ying MA ; Miao HE ; Cheng-Gui ZHANG ; Yue LI
Chinese Pharmacological Bulletin 2018;34(4):496-501
Aim To study the effect of the Kangfuxin liquid on 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced ulcerative colitis in rats, and to explore its mechanism. Methods Male SD rats were divided into normal control, model control, SASP groups, and Kangfuxin low,medium and high dose groups. In ad-dition to the normal control group, other groups were induced ulcerative colitis with TNBS solution. Disease activity index (DAI), organ index, colon mucosa damage index (CMDI) and histopathological score (HS),the expression levels of IL-4, IL-17 in serum, and MPO, EGF, TGF-β1 in colonic mucosa were de-termined. Results The DAI score showed that the model was successful. Compared with the normal group,the level of IL-4 and IL-17, EGF and TGF-β1 in the model group were reduced significantly, while the CMDI score,HS score,colon index and MPO were elevated significantly. The DAI, CMDI, HS and MPO were reduced (P<0.05 or P<0.01), and the levels of IL-4, IL-17, EGF and TGF-β1 increased signifi-cantly(P <0.01). Conclusions Kangfuxin liquid can effectively alleviate ulcerative colitis induced by TNBS in rats. The mechanism may be related to the down-regulation of MPO expression and up-regulation of IL-4,IL-17,EGF and TGF-β1 levels.
2.Advances in Research on Active Ingredients and Related Pharmacology of Periplaneta americana
Yang-yang GAO ; Fu-neng GENG ; Si-min CHEN ; Xiu-ying MA
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(4):240-250
3.Analysis of Material Basis and Mechanism of Sangjiang Ganmao Injection in Treatment of Common Cold Based on UPLC-Q-Orbitrap HRMS and Network Pharmacology
Cheng-yi PENG ; Yi-jia ZENG ; Hai-jun XIONG ; Fu-neng GENG ; Qin-wan HUANG ; Da-yong ZHANG
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(14):155-163
Objective:To explore the material basis and mechanism of Sangjiang Ganmao injection (SG) in the treatment of common cold by ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) and network pharmacology. Method:UPLC-Q-Orbitrap HRMS was used to identify the chemical components of SG with mobile phase of acetonitrile (A)-0.1% formic acid aqueous solution (B) for gradient elution (0-10 min, 4%-15%A; 10-35 min, 15%-30%A; 35-45 min, 30%-33%A; 45-55 min, 33%-60%A; 55-58 min, 60%A), flow rate of 0.2 mL·min-1, electrospray ionization (ESI) and scanning range of
4.Establishment and Application of Identification Method for Fishy Odor of Cordyceps Based on HS-SPME/GC-QQQ-MS/MS
Peng TAN ; Wei ZHU ; Xiao-ming BAO ; Fu-neng GENG ; Yong-sheng WEN ; Ding-kun ZHANG
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(7):100-111
Objective:To establish and apply a new practical analytical method for identifying the fishy odor of Cordyceps based on headspace-solid phase microextraction-gas chromatography-triple quadrupole mass spectrometry (HS-SPME/GC-QQQ-MS/MS) technique. Method:The InertCap Pure-WAX capillary column (0.25 mm×30 m, 0.25 μm) was used for chromatographic separation. The injection port temperature was set at 250 ℃. The injection mode was split injection with a ratio of 5∶1. High purity helium was used as the carrier gas and control mode was set to constant pressure. The column flow rate was 1.43 mL∙min-1, the linear velocity was 43.3 cm∙s-1, and the purge flow rate was 3.0 mL∙min-1. The chromatographic column temperature program as follows:maintained the initial temperature at 50 ℃ for 5 min, and increased the temperature at a rate of 10 ℃∙min-1 to 250 ℃, held for 10 min. The column equilibrium time was 2.0 min. The ion source of mass spectrographic analysis was electron ionization with ion source temperature of 200 ℃, and the monitoring mode was set to multiple reaction monitoring. Result:Seven batches of Cordyceps samples were collected, including 3 batches from Sichuan, 3 batches from Qinghai and 1 batch from Tibet. There were six batches of counterfeits, including 3 batches from Sichuan, 2 batches from Guizhou and 1 batch in Xinjiang. A total of 81 volatile compounds were screened out in Cordyceps, which could be divided into 13 types (esters, ketones, aldehydes and others) according to the compound structure, indicating that the fishy odor of Cordyceps was a complex odor. There was no significant difference in the types of volatile compounds of Cordyceps from different regions, which suggested that these volatile compounds in Cordyceps produced in Tibet (Naqu), Qinghai (Yushu and Guoluo) and Sichuan (Litang, Rangtang and Seda) were relatively consistent. However, the contents of some volatile compounds in Cordyceps produced in different regions were quite different, and 16 volatile compounds with significant difference were screened out, including 1-methoxy-2-propyl acetate,
5.Research progress on triptolide functionalized nanoparticulate drug delivery systems for tumor treatment.
Yao-Yao LUO ; Jin-Feng SHI ; Liang CHEN ; Jia-Xin LI ; Xiu-Ying MA ; Jin-Ming ZHANG ; Fu-Neng GENG
China Journal of Chinese Materia Medica 2019;44(21):4566-4572
Nowadays,the advantages of traditional Chinese medicine(TCM) for treatment of tumors are increasingly prominent.Triptolide shows wide-spectrum and highly effective anti-tumor activity. Moreover,nano-carrier-based triptolide drug delivery system is more powerful in improving water solubility and pharmacokinetic behavior of the drug,but it is easy to cause toxic and side effects that should not be neglected on human body. Because of tumor vascular heterogeneity and PEGylation dilemma,nanoparticulate drug delivery systems need to overcome multiple physiological and pathological barriers from drug administration to functioning. It is difficult for traditional triptolide nanoparticulate drug delivery systems to achieve active accumulation of nano-drug in tumor tissues and specific drug release in tumor target site solely relying on enhanced permeability and retention effect of solid tumor,limiting their application and clinical transformation in treatment of tumors. Based on the traditional nano-preparation system,the new functionalized nano-drug delivery system further enhances the nano-drug enrichment,penetration and controlled release at the tumor sites,which is of great significance in improving bioavailability,anti-tumor efficacy and reducing the side effects of drugs. In this paper,we summarized and analyzed the researches on new triptolide functionalized nano-drug delivery system from four perspectives,including tumor active targeting,tumor microenvironment response,polymer-drug conjugates,and multidrug co-delivery for tumor treatment,expecting to provide ideas for in-depth research and clinical application of triptolide and some other active anti-tumor TCM ingredients.
Diterpenes/chemistry*
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Drug Delivery Systems
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Epoxy Compounds/chemistry*
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Humans
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Nanoparticles
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Phenanthrenes/chemistry*
6.Preparation of Periplaneta americana Thermosensitive Hydrogel and Investigation of Its Wound Healing Effect on Diabetic Rats
Yao-yao LUO ; Qian LIAO ; Ting WANG ; Xiu-ying MA ; Yang-yang GAO ; Jie LI ; Jin-feng SHI ; Chao-mei FU ; Jin-ming ZHANG ; Fu-neng GENG
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(14):164-172
Objective:To prepare
7.Physicochemical properties and anti-inflammatory and immunomodulatory effects of Shengfupian polysaccharides.
Qi HU ; Yu LIU ; Li HAN ; Yu-Sen HOU ; Chen-Juan ZENG ; Fu-Neng GENG ; Ming YANG ; Ya-Nan HE ; Ding-Kun ZHANG
China Journal of Chinese Materia Medica 2023;48(10):2757-2766
In this study, the crude polysaccharides was extracted from Shengfupian and purified by Sevag deproteinization. Then, the purified neutral polysaccharide fragment was obtained by the DEAE-52 cellulose chromatography column and Sephadex G-100 co-lumn. The structure of polysaccharides was characterized by ultraviolet spectroscopy, infrared spectroscopy, ion chromatography, and gel permeation chromatography. To investigate the anti-inflammatory activity of Shengfupian polysaccharides, LPS was used to induce inflammation in RAW264.7 cells. The expression of the CD86 antibody on surface of M1 cells, the function of macrophages, and the content of NO and IL-6 in the supernatant were examined. An immunodepression model of H22 tumor-bearing mice was established, and the immunomodulatory activity of Shengfupian polysaccharides was evaluated based on the tumor inhibition rate, immune organ index and function, and serum cytokine levels. Research indicated that Shengfupian polysaccharides(80 251 Da) was composed of arabinose, galactose, glucose, and fructose with molar ratio of 0.004∶0.018∶0.913∶0.065. It was smooth and lumpy under the scanning electron microscope. In the concentration range of 25-200 μg·mL~(-1), Shengfupian polysaccharides exhibited little or no toxicity to RAW264.7 cells and could inhibit the polarization of cells to the M1 type and reduce the content of NO and IL-6 in the cell supernatant. It could suppress the phagocytosis of cells at the concentration of 25 μg·mL~(-1), while enhancing the phagocytosis of RAW264.7 cells within the concentration range of 100-200 μg·mL~(-1). The 200 mg·kg~(-1) Shengfupian polysaccharides could alleviate the spleen injury caused by cyclophosphamide, increase the levels of IL-1β and IL-6, and decrease the level of TNF-α in the serum of mice. In conclusion, Shengfupian polysaccharides has anti-inflammatory effect and weak immunomodulatory effect, which may the material basis of Aconm Lateralis Radix Praeparaia for dispelling cold and relieving pain.
Animals
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Mice
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Interleukin-6/genetics*
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Cytokines/metabolism*
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Polysaccharides/chemistry*
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RAW 264.7 Cells
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Anti-Inflammatory Agents/chemistry*
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Spectrophotometry, Infrared
8.Stems and leaves of Aconitum carmichaelii Debx. as potential herbal resources for treating rheumatoid arthritis: Chemical analysis, toxicity and activity evaluation.
Ya-Nan HE ; Shui-Ping OU ; Xi XIONG ; Yuan PAN ; Jin PEI ; Run-Chun XU ; Fu-Neng GENG ; Li HAN ; Ding-Kun ZHANG ; Ming YANG
Chinese Journal of Natural Medicines (English Ed.) 2018;16(9):644-652
According to folk usage of Aconitum carmichaelii Debx., the present study was designed to determine the feasibility of the stems and leaves of Aconitum carmichaelii Debx. as a new medicinal resource. Fourteen alkaloids in mother roots, fibrous roots, stems, and leaves of Aconitum carmichaelii Debx. were measured by HPLC-MS/MS. And multivariate analysis methods, such as clustering analysis and principal component analysis, were applied to analyze the difference among various parts. In addition, the acute toxicity, analgesia, and anti-inflammatory tests were carried out. The results suggested that the contents of alkaloids in mother roots and fibrous roots were approximate, but those of leaves and stems were different from mother roots and fibrous roots. The results of the acute toxicity testing demonstrated the toxicity of fibrous root was strongest, and mother roots were slightly less toxic than fibrous roots. The stems and leaves were far less toxic than mother and fibrous roots. In addition, the analgesia and inflammatory tests showed the effects of the various tissues had no difference each other. These results provided a basis for developing new complementary and alternative treatments for rheumatoid arthritis patients. Simultaneously, the approach may also turn wastes into treasure and promote the development of circular economy.
Aconitum
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chemistry
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Alkaloids
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administration & dosage
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chemistry
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toxicity
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Animals
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Anti-Inflammatory Agents
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administration & dosage
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chemistry
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toxicity
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Arthritis, Rheumatoid
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drug therapy
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Chromatography, High Pressure Liquid
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Drug Evaluation, Preclinical
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Drugs, Chinese Herbal
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administration & dosage
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chemistry
;
toxicity
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Female
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Humans
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Male
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Mice
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Plant Leaves
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chemistry
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Plant Roots
;
chemistry
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Plant Stems
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chemistry
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Tandem Mass Spectrometry