3.Effects of Trillim tschonskii maxim on microvessels response and anti-oxidation enzyme in burned rats.
Chinese Journal of Applied Physiology 2011;27(4):483-494
Animals
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Burns
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drug therapy
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metabolism
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physiopathology
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Drugs, Chinese Herbal
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pharmacology
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therapeutic use
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Female
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Male
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Mesentery
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blood supply
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Microvessels
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drug effects
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physiopathology
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Phytotherapy
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Rats
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Rats, Sprague-Dawley
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Superoxide Dismutase
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metabolism
;
Trillium
;
chemistry
4.Effects of pulmonary arterial endothelium cells injuryed by tumor necrosis factor ? on proliferation of pulmonary arterial smooth muscle cells and the interference effects of heat stress response
Ling PEI ; Junke WANG ; Wen FU
Chinese Journal of Anesthesiology 1994;0(01):-
Objective To investigate the effects of pulmonary arterial endothelium cells(PAEC) injuryed by tumor necrosis factor ? (TNF-?) on the proliferation of pulmonary arterial smooth muscle cells (PASMC) and the influence of heat stress response (HSR) on it .Methods Normal PAEC or PAEC endured with HSR were incubated with TNF-? at the concentrations of 500, 1000 and 2000u/ml in 1 hour, then cultured in DMEM without serum in 24 hours, the upper liquid was collected to prepare the endothelial cell-conditioned medium liquid (EC-CMⅠ or EC-CMⅡ ), in which PASMC were incubated in 24 hours as group Ⅰ or group Ⅱ respectively. The normal endothelial cell-conditioned medium liquid was also prepared to incubate PASMC in 24 hours as group Ⅲ.The PASMC were incubated without the endothelial cell-conditioned medium liquid as group Ⅳ.Flow cytometry was applied to determining the intracellular DNA content of the incubated PASMC. The fractions of different cytocycle phases were calculated according to the areas under the curves of DNA content.Results Compared with those of group Ⅳ, the percentage of PASMC in G0-G1 phases increased markedly ,and in S and G2-M phases decreased significantly in group Ⅲ (P
5.Neuromuscular effects and safety of an intravenous infusion of rapacuronium under inhalation or intravenous anesthesia
Wen FU ; Junke WANG ; Ling PEI
Chinese Journal of Anesthesiology 1994;0(04):-
0 05) The duration of T 1 recoving to 90% of baseline was 34 57min,and the recovery index was 24 29 min No any histamine related side effects were observed in all patients Conclusions Intravenous infusion of rapacuronium can produce safe and effective neuromuscular blockasde during desflurane, sevoflurane, isoflurane, or propofol anesthesia After the rapacuronium infusion of 45 60 min, the recovery from the neuromuscular blockasde is prolonged
6.The effect of gingkgo biloba on cytokine levels and clinical efficacy in treatment of patients with asthma
Aishuang FU ; Ling WANG ; Yaning ZHAO ; Hongyang WANG
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2014;(5):328-330
ObjectiveTo observe the effect of extract of ginkgo biloba(EGB) on cytokine levels and clinical efficacy of patients with asthma.Methods A prospective randomized control trial was conducted. 112 patients with asthma in Department of Respiratory Medicine of Affiliated Hospital of Hebei Union University were enrolled. The patients were divided into ginkgo lamina group(58 cases) and conventional therapy group(54 cases) by random number table.According to the Global Initiative for Asthma(GINA) Prevention and Treatment, both groups received conventional therapy, and in addition, every patient in ginkgo lamina group took 2 tablets once of EGB(40 mg/tablet) orally, 3 times a day for 2 weeks. The radioimmunoassay was used to detect the levels of tumor necrosis factor-α(TNF-α), interleukins(IL-6, IL-8, IL-10) in patients of two groups before and after treatment, and the changes of pulmonary functions were observed.Results After treatment, serum IL-6,IL-8 and TNF-α were significantly decreased and IL-10 was increased in patients of both groups, the above changes of indexes in ginkgo lamina group being more obvious compared with those in conventional therapy group〔IL-6(μg/L): 0.15±0.05 vs. 0.23±0.12, IL-8 (μg/L): 0.51±0.24 vs. 1.42±0.54, IL-10(μg/L): 69.18±13.12 vs. 32.61±12.51, TNF-α(μg/L): 1.35±0.59 vs. 2.14±1.52,allP<0.05〕. After treatment, the pulmonary function indexes of patients in the two groups were increased obviously, and the increase in ginkgo biloba group being more significant compared with that in conventional therapy group〔percentage of 1 second forced expiratory volume/predicted value(FEV1%):(68.12±0.38)% vs. (55.32±0.24)%, FEV1/forced vital capacity(FEV1/FVC):(71.32±0.59)% vs.(56.56±0.42)%, percentage of peak expiratory flow rate/predicted value(PEF%):(63.28±0.24)% vs.(52.14±0.24)%, allP<0.05〕. ConclusionGinkgo biloba may improve the airway inflammation in patients with asthma by affecting the levels of serum IL-6, IL-8, IL-10 and TNF-α.
7.Study on pure red cell aplastic lymphocyte subsets characteristics and the effect of cyclosporin A
Zengsheng WANG ; Qin HUANG ; Hong LIU ; Ling FU ; Xiaomin WANG
Cancer Research and Clinic 2015;27(8):526-528
Objective To detect and analyse acquired pure red cell aplasia (PRCA) T lymphocyte subsets distribution and to assess its condition and cyclosporine immune function of T lymphocytes subsets.Methods Flow cytometry was applicated to detect peripheral blood T lymphocyte subsets of acquired PRCA patients before and after 3 months of treatment with cyclosporine-based immunosuppressive regimen and normal controls.Results Among 22 patients,17 cases of blood returning normal (three cases of bone marrow returning normal),the total effective rate was 95.5 % (3 cases of cure,14 cases of remission,4 cases of improvement,1 case of ineffectiveness).Th (CD3+ CD4+) cells and Th/Ts ratios in acquired PRCA patient group were lower than those in the normal control group,while Ts (CD3+ CD8+) cells was higher than that in the normal control group,the differences were statistically significant (P < 0.05).After 3 months of treatment,Th cells and Th/Ts ratio were higher than those before,and Ts cells were decreased compared with the previous (P < 0.05).Conclusion Disorders of T lymphocyte subsets in acquired PRCA patients lead to immune dysfunction,however,cyclosporine can improve T lymphocyte subsets in patients with imbalance,which is an effective way to treat the disease with significant curative effect and mild adverse reactions.
9.Consideration and Discussion on Establishment ofStandardized Manipulations of Acupuncture and Moxibustion
Jing WANG ; Ling LUO ; Yong TANG ; Shuguang YU ; Wenbin FU
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(6):1310-1314
TheStandardized Manipulations of Acupuncture and Moxibustion has been published and gradually standardized the clinical practice. It provided a solid foundation for the standardization of acupuncture and moxibustion therapeutic effects. It also provided powerful convictable effects on the accuracy and scientificity of acupuncture and moxibustion clinical practice, which gained well-acceptance from patients. In the treatment of certain diseases, acupuncture and moxibustion have become irreplaceable so that acupuncture and moxibustion can be strongly promoted both at home and abroad. There were some common problems occurred during the establishment of the national standardization onStandardized Manipulations of Oral and Lip Acupuncture. This article reviewed, analyzed and discussed questions occurred during the standardization establishment. The results showed that according to the principle of standardized production, a rigorous, scientific and reasonable text standard can be established. Advices or opinions gained during the process can be used as references for future standardization establishment. It was concluded that the standardization of acupuncture had great guidance in the acupuncture clinical application. It is an arduous and long-term mission to achieve and strengthen the position of international standardization of acupuncture and moxibustion. The government should increase the effort on investment and promotion of acupuncture and moxibustion standardization. Conversation and cooperation should also be strengthened with international communities to promote the standardization implementation.
10.Evaluation of the application of regional citrate anticoagulation in sustained low efficiency hemodialysis
Tingli WANG ; Ling ZHANG ; Zhiwen CHEN ; Ping FU
Chinese Journal of Internal Medicine 2014;53(12):953-956
Objective To compare the anticoagulation effect of regional citrate and heparin in patients with sustained low-efficiency hemodialysis (SLED).Method This study was conducted in the teaching hospital of Sichuan University between November 2011 and January 2013.Sixty-three patients suffering from acute kidney injury or end-stage renal diseases (ESRD) were enrolled and further randomized to 2 groups:citrate and heparin anticoagulation treatment groups in SLED.SLED was conducted by Fresenius 4008sARrTplus dialyzer for 8 hours each session,and blood flow was set at 150 ml/min.Prothrombin time (PT),activated partial thromboplastin time (APTT) and platelet (PLT) count were analyzed.Result Sixty-three patients underwent 118 sessions of SLED.Among them,59 patients (93.7%) was discharged after treatment or converted to outpatient intermittent hemodialysis,and 4 patients died of multiple organ failure during hospitalization.Compared with that in the citrate group,both PT and APTT in heparin group was significantly higher [PT:(15.5 ± 2.0) s vs (12.3 ± 2.7) s,P < 0.001 ; APTF:(56.0 ± 10.9) s vs (32.8 ± 6.1) s,P < 0.001 ; respectively] at 2 h during SLED.However,the PT and APTT levels in heparin group decreased afterwards and were similar with those in the citrate groups at 5 h during treatment.There is no difference on PLT counts between these two groups after treatment.Conclusion The anticoagulation effect of regional citrate and heparin was similar in patients when receiving SLED.Regional citrate may be an alternative anticoagulant approach for the patients at high risk of bleeding who require the treatment of SLED.