OBJECTIVETo study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-kappaB) cis-elements on expression of inflammation mediators in pMPhi cells from rats.

METHODSWith carriers of cationic liposomes, decoy-ODNs were transfected into pMPhi cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and IL-10 were analyzed.

RESULTSDecoy-ODNs could decrease the expression of TNFalpha and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10.

CONCLUSIONSDecoy-ODNs targeting NF-kappaB can decrease the expression of inflammatory mediators in pMPhi cells from rats.

Animals ; Base Sequence ; Cells, Cultured ; Dose-Response Relationship, Drug ; Female ; Inflammation Mediators ; metabolism ; Interleukin-10 ; metabolism ; Interleukin-6 ; metabolism ; Male ; Molecular Sequence Data ; NF-kappa B ; drug effects ; metabolism ; Oligodeoxyribonucleotides ; pharmacology ; RNA, Messenger ; analysis ; Random Allocation ; Rats ; Rats, Wistar ; Reference Values ; Reverse Transcriptase Polymerase Chain Reaction ; methods ; Sensitivity and Specificity ; Tumor Necrosis Factor-alpha ; drug effects ; metabolism