1.Research progress in cancer genomics.
Chinese Journal of Oncology 2006;28(7):555-557
3.Genetic analysis of complex diseases: status quo and prospects.
Acta Academiae Medicinae Sinicae 2009;31(6):661-663
This review comments the status quo, especially the major problems, of the genetic analysis of the complex diseases, provides some possible solutions, and explores the further development trends in this field.
Genetic Testing
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Humans
4.Peroxisome proliferator activated receptor gamma Pro12Ala: old topic of conversation and new question.
Acta Academiae Medicinae Sinicae 2011;33(6):591-592
Comments concerning Meta analysis for relationship between peroxisome proliferator activated receptor gamma Pro12Ala polymorphism and type 2 diabetes susceptibility in different cohorts in this mini review were given. The comments pointed out existent problems and presented suggestions for genetic analysis of diseases in Chinese populations.
Asian Continental Ancestry Group
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genetics
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Diabetes Mellitus, Type 2
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genetics
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Genetic Predisposition to Disease
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Humans
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PPAR gamma
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genetics
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Polymorphism, Genetic
5.New trends in research on cancer genomics.
Chinese Journal of Oncology 2007;29(7):544-544
DNA Methylation
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DNA, Neoplasm
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genetics
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Genomics
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trends
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Human Genome Project
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Humans
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Neoplasms
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genetics
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Research
7.Study on serological cross-reactivity of six pathogenic phleboviruses.
Wei WU ; Shuo ZHANG ; Quan-Fu ZHANG ; Chuan LI ; Mi-Fang LIANG ; De-Xin LI
Chinese Journal of Virology 2014;30(4):387-390
This article aimed to study the antigenicity of nucleocapsid proteins (NPs) in six pathogenic phleboviruses and to provide theoretical evidence for the development of serological diagnostic reagents. NPs of six pathogenic phleboviruses were expressed and purified using a prokaryotic expression system and rabbits were immunized with individual recombinant NPs. Cross-reactions among NPs and rabbit sera were determined by both indirect ELISA and Western blotting analyses, and the sera titer was determined by indirect ELISA. Furthermore, sera from SFTS patients were also detected by each recombinant NP as a coating antigen using indirect ELISA. The cross-reactions and the sera titer were subsequently determined. Both the concentration and purity of recombinant NPs of six pathogenic phleboviruses met the standards for immunization and detection. The results of indirect ELISA and Western blotting showed that each anti-phlebovirus NP rabbit immune serum had potential serological cross-reactivity with the other five virus NP antigens. Furthermore, the sera from SFTS patients also had cross-reactivity with the other five NP antigens to a certain extent. Our preliminary study evaluated the antigenicity and immune reactivity of six pathogenic phleboviruses NPs and laid the foundation for the development of diagnostic reagents.
Animals
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Antibodies, Viral
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immunology
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Antigens, Viral
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genetics
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immunology
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Cross Reactions
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Humans
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Nucleocapsid Proteins
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genetics
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immunology
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Phlebotomus Fever
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diagnosis
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immunology
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virology
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Phlebovirus
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classification
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genetics
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immunology
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isolation & purification
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Rabbits
8.Cloning , Expression of Human sTNFR1 Gene and the Biological Activity of Its Recombinant Protein
Lei FU ; Shi-Fang PENG ; De-Ming TAN ; Hong-Bo LIU ;
China Biotechnology 2006;0(07):-
Human sTNFR1 (soluble tumor necrosis factor receptor 1) gene was amplified by RT-PCR from Hela cells. A recombinant expression vector of sTNFR1-MBP was constructed in pMAL-c2x, and transformed into E. Coli JM109.It was sequenced and confirmed to be identifical to the sTNFR1 gene in data bank. Recombinant protein sTNFR1-MBP was induced by IPTG and purified by Amylose resin Affinity Chromatography. sTNFR1-MBP was binded to sTNFR1's antibody in Western-blotting. From MTT assays, the results showed that sTNFR1-MBP could effectively block the cytotoxicity mediated by TNF?on QSG7701 cells. Annexin V-FITC staining and flowcytometry were used to observe the recombinant protein's anti-apoptosis capacity and the recombinant protein has marked anti-apoptosis effect in vitro.sTNFR1-MBP had good biological activity and it will be employed in further study.
10.Virus-like particle-based immunoglobulin M capture enzyme-linked immunosorbent assay for the detection of IgM antibodies against Chikungunya virus.
Jian-dong LI ; Quan-fu ZHANG ; Shuo ZHANG ; Chuan LI ; Qin-zhi LIU ; Mi-fang LIANG ; De-xin LI
Chinese Journal of Virology 2014;30(6):599-604
To establish a MacELISA method for the detection of IgM antibodies against Chikungunya virus (CHIKV), we prepared virus like particle (VLP) antigens of CHIKV using the whole structural protein C-E3-E2-6K-E1 encoding gene with a baculovirus expression system in Sf9 insect cells. The VLPs were purified and used to immunize Kunming mice. Then, polyclonal antibodies were purified from the samples of ascites with a protein G HiTrap SP column and labeled with horseradish peroxidase. A MacELISA method for the detection of IgM antibodies against CHIKV was assembled with goat anti-human IgM antibody, VLP antigens and an enzyme-labeled polyclonal antibody. The results were evaluated with a serum panel containing serum samples from laboratory-confirmed CHIK, HFRS patients, healthy donors, and commercially available CHIKV IgM as a quality control. It was shown that the MacELISA had a specificity of 99% (99/100), the coefficients of variation (CoV) within a plate were <10%, and the CoV of different ELISA plates in terms of the plate variation coefficient was <15%. A comparative analysis was performed to compare the current method against a commercial CHIKV IgM antibody detection kit for IIFA-IgM. The detection limit of MacELISA was significantly lower than that of the IIFA-IgM commercial kit (P< 0.0001). Here, we demonstrate that the VLP-based MacELISA is a promising tool for the early diagnosis and epidemiological investigation of CHIKV infection, with a high level of sensitivity and specificity for the detection of IgM antibodies against CHIKV.
Animals
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Antibodies, Viral
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blood
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Chikungunya Fever
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blood
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diagnosis
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virology
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Chikungunya virus
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immunology
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isolation & purification
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Enzyme-Linked Immunosorbent Assay
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methods
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Humans
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Immunoglobulin M
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blood
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Mice