Objective To investigate the risk factors that may affect postoperative complications in patients with meningiomas. Methods Of all 300 patients with diagnosed pathologically intracranial meningiomas undergoing resection were retrospectively ana-lyzed in terms of clinical datas and radiological features of preoperative MRI.These patients were divided into two groups according to whether postoperative complications occurred after tumor resection.Both univariate and multivariate regression models were used to analyze the effect of patient age and several MRI characteristics on postoperative complications.Results There were several signif-icant differences between the two groups in patient age,tumor location,tumor size,shape of tumor,wrapping around nerves and blood vessels,and tumor enhancement (P <0.05).According to the results of multivariate Logistic regression analysis,tumor loca-tion,tumor size,wrapping around nerves and blood vessels,and tumor enhancement were independently identified risk factors in the prediction of postoperative complications associated with meningioma resection.Conclusion The features of preoperative MRI may be useful for clinicians in predicting postoperative complications of intracranial meningiomas,and can provide imaging evdience for effective treatment and prognostic assessment in patients with meningiomas.

Objective:To examine whether the existence of the donor-and-recipient-derived DNA chimerism in recipient’s plasma can be a predictive marker for the status of transplanted organ.Methods:One hundred and twenty-six female patients who had been transplanted with male kidney were enrolled in the present study.In these female recipients,the SRY1,DYZ11st and DYZ12nd genes on the Y chromosome from the plasma were prospectively examined using reverse transcription polymerase chain reaction (RT-PCR).Results:SRY1,DYZ11st and DYZ12nd sequences were detected in the cell-free blood (plasma) of 97 (77%) of 126 female patients with male kidney.The average time-span when the transplanted kidneys functioned was 8.7 years and 5.4 years among microchimerism-positive and microchimerism-negative recipients,respectively.The frequency of the patients who had acute rejection after renal transplantation was approximately 10% and 28%in microchimerism-positive and microchimerism-negative recipients,respectively.Serum creatinine levels in microchimerism-positive patients were significantly lower than those in microchimerism-negative patients.Conclusion:These results suggest that plasma DNA microchimerism is present in certain patients following renal transplantation and measurement of plasma DNA microchimerism using quantitative RT-PCR might be a useful predictor for the acceptance of transplanted kidneys.

Objective To comprehensively analyse the transcriptional changes of genes and their biologic significance that occurred in the process of development of human fetal skin by using high-density oligonucleotide DNA array. Methods Samples of human fetal skin were obtained from aborted fetuese of 10W, 15W, 24W, 32W EGA (estimated gestational age) respectively. Total RNAs were isolated from of skin specimens of fetuses of different EGA, and mRNAs were purified and labeled with incorporation of fluorescent dUTP to prepare the hybridization probes by using reverse transcription polymerase chain reaction (RT-PCR). Approximately 21 329 human genes were spotted on a chemical-material-coated-glass plate in array. Results According to the hybridization results from oligonucleotide DNA microarray, gene expresion patterns and functions were analysed. Gene-chip disclosed a large scale of information in developmental human fetal skin, rendering a convenient way to investigate the temporal and spatial expressions of gene profile among skin cells. Many specific genes transcription expressed differently at different stages of development of fetal skin, suggesting their key roles in development, differentiation and regulation. Conclusions Microarray or DNA chip technology has revolutioned biological research by empowering to broaden the scope of collecting genomic information. Therefore, microarray-based study is able to reveal a substantial number of genes which might participate in embryogenesis and development of human skin. The present study demonstrated a previously unrecongnized role of gene expression in the control of human fetal skin growth and structure during its developmental stage. A complicated network of skin development process was fairly well characterized.

Object To establish a process for the preparation of QINGXUE JIANGZHI CAPSULE(Hemocathartic Antilipemia Capsule)  Methods The study was carried out by uniform experimental design guided by the content of total organic acid and polysaccharide in the resulting preparation, to optimize the ratio of solvents used for the extraction, time needed for the decoction and alcoholic concentration for precipitation Results The most favorable preparative conditions were: decocting the drug twice with 10 times of water for 60 min each time, and precipitate the product at a concentration of 50% alcohol Conclusion Products prepared by this process ensured the content of total organic acid with no loss of polysaccharides

AIM: To optimize the extraction process of the total flavonoids of Pollen typhae. METHODS: The optimum extraction process was selected with the orthogonal design (L 27 (3 13 )), using the contents of typhaneoside and isorhamnetin 3 O neohesperidoside as the evaluating criteria. RESULTS: The significant effects of alcohol concentration, extraction time and extraction times on the extraction of the total flavonoids of Pollen typhae were discovered. CONCLUSION: The optimum extraction process was as follows: Pollen typhae was extracted with 40% alcohol for two times, each with the solvent volume 14 times of the weight of the raw materials and extraction time for 3 h.

This article was aimed to study the mechanism of interaction between human serum albumin (HSA) and zinc ions, in order to provide the information on the secondary structure modification of HSA and the thermodynamics parameters using circular dichroism (CD) and isothermal titration calorimetry (ITC). CD and ITC were applied in the study. The concentration of HSA were 0.025 mmol·L-1, 0.05 mmol·L-1, 0.1 mmol·L-1, and 0.2 mmol·L-1, respectively. The concentration of zinc ion was 5 mmol·L-1. The CD analysis revealed that the secondary structure modification of HSA differed depending on the concentration of HAS. And the content ofα-helix was inversely proportional to the concentration of HSA. When the concentration of HSA was 0.2 mmol·L-1, the content ofα-helix was special. A series of thermodynamics parameters including association constants (Kb), stoichiometry (N), entropy (ΔS) and enthalpy (ΔH) were investigated by ITC analysis. And two types of binding sites were observed when ZnSO4(mmol·L-1)?HSA (mmol·L-1)= 5?0.2. The secondary structure modification of HSA interacting with zinc ions depended on the concentration of HSA, a dramatic reduction ofα-helix was detected when the concentration of HSA attained 0.2 mmol·L-1. And the protein hydrophobicity reduction and peptide chains expansion were equally observed at this concentration. The ITC analysis also revealed endothermic and exothermic binding sites in the ZnSO4-HSA interaction when ZnSO4 (mmol·L-1)?HSA (mmol·L-1)= 5?0.2, indicating that the endothermic sites were specific but not preferential for zinc ions interactions. These results provided theoretical supports for the application of Zn2+ to open the endothermic sites of HSA.

Objective To identify the miR-122 which regulateing GATA4 expression during the induction of rat bone marrow mesenchymal stem cells (bMSCs) differentiating into cardiomyocyte-like cells. Methods BMSCs were isolat-ed from bone marrow and induced to differentiate into cardiomyocyte-like cells using 5-azacytidine. The miR-122 which may regulate expression of GATA4 were predicted using miRanda and TargetScan softwares and identified by dual luciferase report system. The expressions of miR-122 and GATA4 were determined using q-PCR during the differentiation of bMSCs into cardiomyocyte-like cells. Results The induced cells were completely in contacted with adjoining cells and uniform in shape and aligned parallelly. Cardiac troponin I (cTnI) expression was detected by immunofluorescence cytochemistry. Using dual luciferase reporter system in vitro, miR-122 were proved to be able to effectively inhibit GATA4 expression by binding the 3′UTR of GATA4 mRNA. q-PCR results showed that the expression of miR-122 is negatively correlated with that of GATA4 mRNA transcription. Conclusion These results indicated that miR-122 regulate the expression of GATA4 during the induction of cardiomyocyte-like cells.

Objective To explore time difference attack therapy in Acinetobacter bauamnnii infection. Methods 67 patients with Acinetobacter bauamnnii were divided into two groups. The experimental group were first given Fosfomycin 4 g 5% GS 100 ml iv girt to finish within 60 min and then given Cefperazone/Sulbactam 4 g 0.9% NS 250 ml iv gitt immediately bid. The control group were given: Ampicillin/Sulbactam 3 g 0.9% NS 250 ml iv gitt (tid) + Ciprofloxacin 0.2 g iv girt (bid). The treatment course all was 11 days. Results The overall effective rate of experimental group methods was superior to that of control group(X2 =9.56 ,P =0. 023). Conclusion The Fos-fomycin Cefperazone/Sulbactam time difference attack therapy for the treatment of Acinetobacter the bauamnnii in-fection is a new way.

AIM:To study the differences of basic fibroblast growth factor(bFGF)gene expression in lens epithelial cells (LECs) between fetuses and cataract patients. METHODS: In situ hybridization was used to detect bFGF mRNA in the LECs that were cultured and in tissue sections from fetuses and in the LECs from the anterior capsule of cataract patients. Image analysis was used for the relative quantitative analysis of bFGF mRNA. RESULTS: bFGF gene existed in the LECs that were cultured and in tissue sections from fetuses and in the LECs from the anterior capsule of cataract patients. The integral absorbance for the fetal cultured cells, the fetal tissue sections and the capsule membrane cells of cataract patients were 627.1±268.7, 131.5±42.8 and 79.2±26.3 respectively. The integral absorbance of fetal cultured LECs was significantly higher than that of fetal section LECs (P<0.01). The integral absorbance of cataract LECs was significantly lower than that of fetal LECs (P<0.01). CONCLUSION: The in vitro culture of LECs can improve bFGF gene expression. The bFGF gene expression in fetal LECs is significantly higher than that in cataract LECs.

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