Stem cells offer considerable promise in the repair or replacement of damaged tissue in the body.Mature and functional endothelial cells play a pivotal role in neovascularization and maintenance of blood vessel integrity.Although being the best choice for transplantation endothelial,cells isolated from autologous vessels have been heavily obstacled in medical application due to poor resources and limitted proliferation potential in culture.Thus,stem cells have been the focus of the studies soareh for suitable cell sources.Several types of stem cells have been reported to possess endothelial cell differentiation potential.This article summarized the recent study on the sources,distribution,features-differentiation,animal experiments and clinical use of stem cells.Advantages and disadvantages are compared to provide a reference for the choice of evdothelial celia source.

Objective To compare the effect of atropine sulphate and cyclopentolate hydrochloride in the treatment of youth hyperopia cycloplegic refraction,and provide the basis for a reasonable choice of the youth cycloplegic refraction drugs.Methods Ninety hyperopia children met inclusion criteria with refractive errors (180 eyes) were subjected to cycloplegic refratometry by 1％ cyclopentolate hydrochloride followed with 1％ atropine sulphate eye used gel.The refractive results,pupil size,residual adjustment amount and systemic side reactions were measured and compared by TOPCON.Results The coincidence rate of spherical lens degrees,cylinder lens degrees and axis of astigmatism degrees respectively was 84.44％ (152/180),92.78％ (167/180) and 92.78％ (167/180) between atropine sulphate and cyclopentolate hydrochloride.The spherical lens degrees of cyclopentolate hydrochloride was lower than atropine sulphate,the difference was statistically significant (P ＜0.01),the cylinder lens degrees and axis of astigmatism degrees had no significant difference between two drugs (P＞ 0.05).The pupil size and residual adjustment amount of cyclopentolate hydrochloride had no significant difference with atropine sulphate (P ＞ 0.05).The incidence of adverse reactions of cyclopentolate hydrochloride was 3.33％ (3/90),atropine sulphate was 13.33％ (12/90),the cyclopentolate hydrochloride was significantly lower than atropine sulphate (P ＜ 0.05).Conclusion The cyclopentolate hydrochloride has powerful,fast and secure feature in the treatment of youth hyperopia cycloplegic refraction,has good ciliary muscle paralysis effect,has longer paralysis time,as effective as the atropine sulphate,and has a shorter recovery time and better security,is ideal cycloplegic drugs for youth hyperopia cycloplegic refraction.

Objective To study P300 wave extraction by a new method. Methods According to the respective characteristics of the brain electrical signal and the signal analysis method, the algorithm by Wavelet Transformation (WT) combined with Independent Component Analysis (ICA), abbreviated to WICA, was applied to P300 wave extraction. Results With WT, the target component of the signal could be strengthened, while the non-target component and the noise were weakened which became weak in multi -channel ICA input signal, and then WICA algorithm could effectively separate strong target component. Conclusion WICA algorithm behaves well in P300 wave extraction.

Gene Expression Regulation ; immunology ; Gene Expression Regulation, Viral ; HIV Infections ; genetics ; immunology ; metabolism ; HIV-1 ; genetics ; immunology ; Humans ; Immunity, Innate ; genetics ; immunology

Objective To discuss the core professional values of nursing(CPVN) in modern China and further promote the education of CPVN. Methods 622 clinical nurses and nursing students were re-cruited to participate the questionnaire survey to explore the understanding of CPVN. Results The top five CPVN were responsibility, accountability, aptitude, professionalism and ethics. Conclusions CPVN should be established based on the development of the times and national conditions in order to improve the overall level of nursing profession in China.

Objective To investigate the reliability and feasibility of sentinel lymph node biopsy (SLNB) in papillary thyroid carcinoma ( PTC ) using methylene blue staining techniques.Methods Nineteen patients,older than 45,with PTC were included in the study.No case had clinical evidence of cervical lymph node involvement(cNO).Methylene blue was injected around the tumor during surgery.The stained lymph nodes were dissected.Subtotal thyroidectomy and modified radical neck dissection were performed.Both the bulk specimen and SLN were submitted for routine histology.Results The sentinel lymph nodes( SLN )were identified in 18 cases,with SLN positive in 13 cases.The sensitivity and specificity of SLNB were 86.6％ and 94.4％ respectively.There was 1 case with SLN metastasis in the lateral neck,and 1 case with positive lymph node and negative SLN.Conclusion SLNB is sensitive in detecting cervical lymph node metastasis and has clinical significance in making operative plans for cN0 PTC.

Objective To investigate the variation of vascular cell adhesion molecule-1 (VCAM-1) expression and lipid peroxidation in aorta and plasma of Ins2~ Akita diabetic mice, and to inquire into the relationship between the variation and the cardiovascular diseases in diabetic patients. Methods With the aortic and plasma samples from Ins2Akita diabetic mice, the protein expression levels of VCAM-1 were detected by Western Blot or ELISA, and the levels of lipid peroxides were determined by lipid peroxidation kit. Results Compared with that of normal control, VCAM-1 protein and lipid peroxides levels were significantly elevated in the aorta and plasma of Ins2~ Akita diabetic mice (P

Objective To investigate the effect of clearing away heat and activating blood decoctions on salivary gland function of patients with Sj?gren's Syndromes (SS). Methods 24 cases of SS (SS group) and 7 cases of normal control (normal group) were tested with salivary gland scintigraphy using 99mTCO4-, and 11 cases of patients were re-tested after treatment. Results The introjection fraction (IF) of salivary gland had no statistical difference between the two groups, while the evacuation fraction (EF) was lower in SS group than in normal group (P

2*!cm. Conclusions Large invasive and metastatic carcinoid tend to be malignant, and the prognosis is usually poor, with which a radical resection may help.

Objective To evaluate circumferential mucosectomy for the treatment of prolapsed hemorrhoids. Methods Ten patients with Ⅲ or Ⅳ degree of circular prolapsed hemorrhoids were operated on with circumferential mucosectomy using circular stapler. The mean age was 65 years old with hemorrhoids history of 3～25 years. Results Hemorrhoids in 8 of the 10 patients disappeared immediately after operation and 2 disappeared on the second day postoperatively. 3 patients complained local pain and 6 patients suffered from temporary urine retention after operation. No significant edema or bleeding ensued. The mean postoperative hospital stay was 4 days. No recurrence developed at an averaged 2 months′ follow up. Conclusion The new technique provides a significantly less painful choice of treatment for severely prolapsed circular hemorrhoids. However, the long-term outcome remains to be identified.