1.Mechanical and biodegradable properties of absorbable hydroxyapatite/poly-DL-lactide screws
Rong FU ; Xiaobo YOU ; Zhendong ZHONG ; Yan LI
Chinese Journal of Tissue Engineering Research 2013;(38):6709-6716
BACKGROUND:Through compounding hydroxyapatite and poly-DL-lactide, the mechanical properties, physical and chemical properties of the implants can be enhanced.
OBJECTIVE:To detect the effect of hydroxyapatite/poly-DL-lactide composite internal fixation screws in canine femoral condyle cancel ous bone fracture repair.
METHODS:Forty-two beagle dogs were operated to bilateral femoral condyle fracture models. The left side was fixed using hydroxyapatite/poly-DL-lactide screws as experimental group;while the right side was fixed with pure poly-DL-lactide screws as control group. After 2, 4, 8, 12, 24, 36 and 48 weeks, conditions of fracture were observed using X-ray, femoral and screw specimens were observed histopathological y, and the bending strength and the average molecular weight were detected. The biological absorption rate, intensity decay rate and biodegradation rate were calculated.
RESULTS AND CONCLUSION:After 2-48 weeks, bilateral fractures were fixed wel and new bone grew wel , but the biological absorption rate of the screws in the experimental group was lower than that in the control group (P ≤0.01). In the first 2, 4 weeks, the bending strength of the experimental screws was higher than that of the control screw (P ≤0.05), but the biodegradable speed was slower in the former one (P ≤ 0.05 or P ≤ 0.01). The pathological changes were similar in the two groups. After 48 weeks, the fractures were healed and bone tissue reconstruction was completed. Compared with the pure poly-DL-lactide screws, the hydroxyapatite/poly-DL-lactide composite internal fixation screws have better fixation effects, biocompatibility, mechanical properties and biodegradability.
4.Prevention and Treatment of Ventilator-associated Pneumonia:Their Characteristics
Feng YAN ; Yijing FU ; Wenling LUO ; Rong CUI ; Ruiqin WANG
Chinese Journal of Nosocomiology 2005;0(11):-
50.0% and increased year by year,the rate of aminoglycoside resistance
5.Effect of hydroxycamptothecin on apoptosis-inducing factor (AIF) expression and on AIF translocation in human hepatocellular cancer cell SMMC-7721.
Yu-rong FU ; Zong-yin QIU ; Yu-rong YAN
Chinese Journal of Hepatology 2006;14(4):285-288
OBJECTIVETo study the effect of hydroxycamptothecin (HCPT) on apoptosis-inducing factor (AIF) expression and AIF translocation from mitochondria to the nucleus in human hepatocellular cancer cell SMMC-7721 during apoptosis.
METHODSAfter treatment with 80 mg/ml of HCPT, the cancer cells were stained with A0/EB to monitor their apoptosis. Their mitochondria was examined with electronmicroscopy and the AIF expression of the cells was tested by RT-PCR and Western blot. The translocation of AIF from mitochondria to the nucleus during apoptosis was analyzed by confocal microscopy.
RESULTSSMMC-7721 cells treated with HCPT showed chromatin condensation, nuclear fragmentation and mitochondria swelling. The mRNA and protein expression of AIF in treated and untreated SMMC-7721 cells were not significantly different. However, cells treated with 80 mg/ml HCPT for 6 h or 12 h showed massive translocation of AIF into the nuclei.
CONCLUSIONThese results show the important role the mitochondrial pathway of apoptosis plays in HCPT-induced tumor cell death, at least in SMMC-7721 cells.
Antineoplastic Agents, Phytogenic ; pharmacology ; Apoptosis ; Apoptosis Inducing Factor ; biosynthesis ; genetics ; Camptothecin ; analogs & derivatives ; pharmacology ; Carcinoma, Hepatocellular ; pathology ; Cell Line, Tumor ; Humans ; Liver Neoplasms ; pathology ; Translocation, Genetic
6.Application of TLE1 expression and fluorescence in-situ hybridization in diagnosing poorly differentiated synovial sarcoma.
Rong-jun MAO ; Qi-ming LI ; Hui-qiong FANG ; Fu-lan HAN ; Xun-fu HUANG ; Yan-xing WU ; Min ZENG
Chinese Journal of Pathology 2011;40(6):403-405
12E7 Antigen
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Adolescent
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Adult
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Antigens, CD
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metabolism
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Biomarkers, Tumor
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metabolism
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Brain Neoplasms
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secondary
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Cell Adhesion Molecules
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metabolism
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Child
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Child, Preschool
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Diagnosis, Differential
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Extremities
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Female
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Follow-Up Studies
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Humans
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Immunohistochemistry
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In Situ Hybridization, Fluorescence
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Infant
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Ki-67 Antigen
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metabolism
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Male
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Neuroectodermal Tumors, Primitive
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metabolism
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pathology
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Oncogene Proteins, Fusion
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metabolism
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Repressor Proteins
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metabolism
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Sarcoma, Ewing
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metabolism
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pathology
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Sarcoma, Synovial
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diagnosis
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metabolism
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pathology
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surgery
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Soft Tissue Neoplasms
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diagnosis
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
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Young Adult
7.99Tcm-MDP whole body bone imaging in 25 patients with SAPHO syndrome
Zhan-li, FU ; Yan, FAN ; Jian-hua, ZHANG ; Xu-he, LIAO ; Jing-hui, LIN ; Rong-fu, WANG
Chinese Journal of Nuclear Medicine 2011;31(5):324-327
Objective To investigate the usefulness of 99Tcm-MDP whole body bone scintigraphy (WBBS) in patients with synovitis,acne,pustulosis,hyperostosis,osteitis (SAPHO) syndrome.Methods 99Tcm- MDP WBBS was performed in 25 patients (6 males,19 females,mean age =(55.1 ±9.8) years)with SAPHO syndrome.Bone lesions were classified into five categories:anterior chest wall,spine,mandible,sacroiliac joint,and limbs.The typical scintigraphic manifestations of SAPHO syndrome were summarized and compared to other radiological imaging data.Results Among 25 patients,32% of cases (8/25)were associated with skin lesion; 48% ( 12/25 ) were pathologically diagnosed with chronic nonspecific bone inflammation by bone biopsy.On 99Tcm-MDP WBBS,abnormal metabolic foci at anterior chest wall were found in all cases,most of which located in the sternocostoclavicular region (96%,24/25 ),including sternoclavicular joints (60%,15/25),first costosternal junctions (48%,12/25),and manubriosternal junctions (44%,11/25 ).Only 20% of the patients (5/25) demonstrated the typical scintigraphic characteristic:“bull's head” sign.The second most frequent part was spine (44%,11/25).Appendicular skeleton was affected in 16% (4/25) patients.WBBS also demonstrated additional skeletal lesions in 68% (17/25 ) of the patients,mainly in first costosternal junctions (7 patients),sternoclavicular joints (6 patients),manubriosternal junctions (5 patients) and spine (4 patients).Conclusions Abnormal metabolic foci in sternocostoclavicular region and other imaging manifestations on 99Tcm- MDP WBBS can be used to diagnose,differentiate,and localize the insidious lesion and evaluate the lesion activity in patients with SAPHO syndrome.
8.Changes in Wnt pathway inhibiting factors in nitrosamine-induced esophageal precancerosis lesions and effect of gexia zhuyu decoction.
Wen-Rong SHI ; Yan LIU ; Jin-Dong XIE ; Shi ZHUO ; Chun-Xiang TU ; Zuo-Fu XIE
China Journal of Chinese Materia Medica 2014;39(16):3131-3135
OBJECTIVETo discuss the changes in Wnt pathway inhibiting factors in esophageal precancerosis lesions induced by methyl benzyl nitrosamine (MBNA) and the effect of Gexia Zhuyu decoction.
METHODWistar rats were subcutaneously injected with MBNA (3.5 mg x kg(-1) for twice per week to establish the model. Since the 1st day after the model establishment, they were orally administered with Gexia Zhuyu decoction (16, 8 mg x kg(-1)). At the 10th week, esophageal tissues were collected to observe the pathological changes of esophageal mucosa, detect SFRP1, sFRP4, Axin1, Axin2 and GSK-3β mRNA levels.by fluorescent quantitation PCR analysis and β-catenin protein level by Western blotting.
RESULTBeing induced by MBNA, rats in the model group showed slight atypical hyperplasia in the histopathological examination. Compared with the normal group, Gexia Zhuyu decoction dose high and low groups showed no significant pathomorphological and histological changes. The model group showed lower gene transcription levels of esophageal tissues sFRP1, sFRP4, Axin1 and Axin2 (P < 0.05 or P < 0.01) and higher β-catenin protein expression level (P < 0.01) than the normal control group. The Gexia Zhuyu decoction low dose group showed higher gene transcription levels of esophageal tissues sFRP1, sFRP4, Axin1 and Axin2 (P < 0.05 or P < 0.01) and lower β-catenin protein expression level (P < 0.01) than the normal control group.
CONCLUSIONUp-regulated β-catenin protein level and down-regulated Wnt pathway could enhance Wnt pathway activity of MBNA-induced esophageal precancerous lesions. Gexia Zhuyu decoction could down-regulate the β-catenin protein level and up-regulate the transcription level of Wnt pathway inhibiting factors, but could not block MBNA-induced esophageal precancerosis lesions.
Animals ; Axin Protein ; genetics ; metabolism ; Drugs, Chinese Herbal ; administration & dosage ; Esophageal Diseases ; drug therapy ; genetics ; metabolism ; pathology ; Glycogen Synthase Kinase 3 ; genetics ; metabolism ; Glycogen Synthase Kinase 3 beta ; Humans ; Male ; Necrosis ; Nitrosamines ; adverse effects ; Proteins ; genetics ; metabolism ; Rats ; Rats, Wistar ; Wnt Proteins ; genetics ; metabolism ; Wnt Signaling Pathway ; drug effects
10.Levels of main platelet thrombin receptors in older chronic haemodialysis patients.
Yan LI ; Lin SHEN ; Rui CHEN ; Fu-rong LU ; Jing LI ; Jian-guo LIU
Chinese Medical Journal 2010;123(17):2495-2496
Aged
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Aged, 80 and over
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Blood Platelets
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chemistry
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Humans
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Receptor, PAR-1
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blood
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Receptors, Thrombin
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blood
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Renal Dialysis
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Uremia
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blood
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therapy