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Objective To compare three kinds of double-dosimetry algorithms used in estimating interventional radiology worker's effective dose.Methods TLDs were put in the Alderson Radiation Therapy Phantom (ARTP) dressed with lead apron,lead cap and lead collar.TLDs were put on the front left chest inside the lead apron and on the left outside lead collar,respectively.Radiation scattering models,intensity-modulated radiotherapy body model at one time and CT dose detection model at another time,were put on the operating table.The ARTP was set at the major operator's position exposed to the Xray,mimicking the real interventional radiology operation.Effective doses were estimated on the basis of organ absorbed doses.Effective doses were also obtained by the use of three kinds of double-dosimetry algorithms.The later three effective doses were compared with the first one.Results Two groups of effective doses were obtained,with each including four data.For intensity-modulated radiotherapy body model,effective doses were 0.138 mSv for phantom method,0.097 mSv for NCRP algorithm,0.161 mSv for Niklason's algorithm and 0.173 mSv for Boetticher's algorithm,respectively.For CT dose detection model,effective doses were 0.018,0.013,0.019 and 0.026 mSv,respectively.The result obtained by using the Niklason's algorithm was the closest to that by using phantom method.Conclusions Niklason's algorithm is more accurate and more practical in eatimating interventional radiology worker's effective dose.

0.05) . The CREB-1 protein expression in cortex and hippocampus was significantly up-regulated while that in nucleus accumbens was significantly down-regulated in chronic morphine dependence and abstinence group (groupⅢandⅣ) as compared with control group. The CREB-1 protein expression in nucleus accumbens in groupⅣwas significantly lower than that in groupⅢ. Conclusion Acute morphine dependence and abstinence do not significantly affect CREB-1 protein expression in the brain. The changes in CREB-1 protein expression are different in different brain regions in chronic morphine dependence and abstinence rats.

In the treatment for anterior teeth alignment in 2 case with mild or intermediate anterior teeth crowding,stainless steel wire ben-ding slide rod with the diameter of 0.5 mm was placed on either side of crowding anterior teeth,Ni-Ti open coil springs were attached to the rod,the gap between the teeth was opened and maintained by the springs.The crowding anterior teeth and irregular midline were adjusted to the normal position.

Objective:To study the clinical significance of examining serum pyridinoline cross-linked N-telopeptides of type I collagen(NTx) and serum bone sialoprotein(BSP) in diagnosing bone metastasis of lung cancer and breast cancer.Methods:A total of 105 patients treated in the Oncology Department of Changhai Hospital were included in this study.Patients were divided into 2 groups:bone metastasis(n=50) and non-bone metastasis groups(n=55).The levels of serum NTx and serum BSP were measured by ELISA.Results:The levels of serum NTx and serum BSP in patients with bone metastasis were significantly higher than in those without bone metastasis(P

Animals ; Corticosterone ; pharmacology ; Hippocampus ; drug effects ; physiology ; In Vitro Techniques ; Long-Term Potentiation ; drug effects ; physiology ; Male ; Rats ; Rats, Wistar

BACKGROUND:Ideal tissue-engineered urinary tract should have good mechanical properties to bear long-term attack of urine and excretion. Muscular conduit of urinary tract in static culture exerts poor strength. It is reported that mechanical stimuli promote cellular growth and secretion of extracellular matrix. OBJECTIVE:To investigate the feasibility of constructing tissue-engineered muscular conduit of urinary tract in bioreactor. METHODS:Adipose-derived stem cells were harvested by col agen enzyme method. After series culture and expansion in vitro, flow cytometric analysis was carried out to detect the immunophenotypes of adipose-derived stem cells. Then the cel-polyglycolic acid complex was constructed by seeding adipose-derived stem cells on polyglycolic acid fibers. After 1 week of in vitro culture, cel-polyglycolic acid complex was cultured in a bioreactor. The experimental group was subjected to pulsatile stimuli, while the control group was cultured in static state. After 3 weeks of in vitro culture in basic medium, the cel-polyglycolic acid complex was induced in the induced culture medium for 4 weeks, and then engineered tissue was examined both grossly and histological y. RESULTS AND CONCLUSION:Flow cytometry demonstrated that the adipose-derived stem cells expressed CD90 (99.42%), CD44 (98.12%) and CD105 (93.27%), but not CD34 (4.92%) or CD45 (0.38%). In the experimental group, tissue-engineered muscular conduit of urinary tract appeared bright color with a round lumen. Immunohistochemical staining showed that after cel-polyglycolic acid complex was induced for 4 weeks, the cells expressed desmin and α-smooth muscle actin. More col agen was found in the complex. In contrast, the control group appeared pale surface and its lumen col apsed slightly. Less col agen was in the complex. Tissue-engineered muscular conduit of urinary tract with good structure can be constructed in a bioreactor.

[Objective]To study the biomechanics of the femoral neck on the treatment of ischemic of the femoral head in dogs using titanium alloy cage,single core decompression and normal.[Method]Twenty-one couples of fresh moist dogs ' femur specimens were divided randomly into 3 groups.In 2 groups,biomechanical test of the femoral neck was made on one of every couple in different conditions:core decompression,core decompression with titanium alloy cage implanting.The other of every couple was tested as normal control.In the third group,one of every couple was tested in condition of core decompression with titanium alloy cage implanting.The other of every couple was tested in condition of core decompression.[Result]The stiffness and the max load of the single core decompression were less than that of the normal control.The max load of femoral head after titanium alloy cage implanting were lager than that of the core decompression or the normal control.[Conclusion]The titanium alloy cage implanting could increase mechanical support for femoral neck significantly and prevent femoral neck broken after manipulation.

Objective To investigate the changes in CREB_1 proteins in five brain regions of rats with morphine addiction and withdrawal with the technique of immunohistochemistry. Methods Thirty six adult male Sprague-Dawley rats were randomly divided into six groups (n=6 for each), i.e. acute morphine dependent group, acute abstinence group, acute control group, chronic morphine dependent group, chronic abstinence group and chronic control group. Animals in dependent groups and abstinence groups were administered with morphine by intraperitoneal injection till morphine dependent models were established. The rats in abstinence groups withdrawal syndromes were induced with naloxone 5mg/kg for 30min. The rats in control groups were injected with saline. All rats were sacrificed by decapitation. The coronal sections of discrete brain regions (hypothalamus, nucleus accumbens, prefrontal cortex, locus coeruleus, hippocampus) were obtained. The relative concentrations of CREB_1 protein were determined with immunohistochemistry. Results In acute morphine dependent and abstinence groups, CREB_1 protein decreased significantly compared with the acute control group in locus coeruleus (P0.05). Conclusion The morphine-induced CREB_1 protein changes may reflect differential G protein-cAMP-CREB signal transduction pathways in morphine dependent and abstinence rats.

Objective The molecular basis for opiate tolerance and dependence remains poorly understood despite extensive investigation in several preparations, including the hippocampus. Recent studies have implicated that the hippocampus played a central role in opiate tolerance, dependence and withdrawal. The current study is to explore the change in guanine nucleotide binding protein-inhabitant (Gi_2) protein in primary cultured hippocampal neurons with morphine treament. Methods The hippocampus was harvested from newborn Sprague-Dawley rats. Primary hippocampal neuronal cultures of 7 days in vitro were used and divided randomly into six groups (n=6), i.e. morphine treatment 4h group (M4), 8h group (M8), 16h group (M16), 24h group (M24), 48h group (M48) and control group (C). All morphine treatment groups were treated with morphine (10?mol/L). C group was treated with saline. The G protein levels were determined with immunofluorscence and laser scanning confocal microscope (LSCM) imaging techniques. Results Gi_2 protein levels in M16, M24 and M48 groups decreased significantly compared with that in C group (P0.05). Among M16, M24 and M48 groups, Gi_2 protein level was lowest in the M48 group. Conclusion The results indicated that Gi_2 protein levels decreased significantly in primary cultured hippocampal neurons with morphine treatment, which might be a potential molecular mechanism of opioid tolerance and dependence.