Adolescent ; Adult ; Aged ; Carbon-Nitrogen Ligases ; genetics ; Female ; Gene Expression ; Humans ; Male ; Middle Aged ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Nasopharynx ; pathology ; Young Adult

10.3969/j.issn.1000-3606.2013.06.003

Objective To explore the dose and effects of mixtures of pethidine and haloperidol for Postoperative Deliri-um. Methods Retrospective analysis was conducted on treatment dose and effects of 85 cases of patients with deliri-um during narcotic awake period or 1 day to 2 days after operation by mixtures (haloperidol with pethidine) from 2005 to 2006 in Affiliated Hospital of Hainan Medical College. Results 1/4 dose of mixture (pethidine 100 mg and haloperidol 5 mg) cured 9 patients, 1/2 dose of mixture cured 61 cases, we add 1/4 dose of mixture when 10 cases occurred delirium again ,5 cases added 1/2 dose of mixture again, all were cured. After administration ,aublood pres-sure declined all, but all were within normal range, the 17 cases's pulse oxygen saturation is less than 95%, Conclu-sion The mixture (haloperidol with pethidine) can be effective treatment for delirium during awake period of general anesthesia or after operation. However, application of low dose therapy from the beginning, and attention to its impact on respiration.

Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Humans ; Middle Aged ; Pneumoconiosis ; epidemiology

A descriptive study to investigate the situation of cross-cultural adaptation and health status among medical international students was carried out using cluster sampling method and self-designed questionnaire in Dalian Medical University.148 international students come from 24 countries.There are 5 religions belief among these students.Islam,Catholic,Hindu,Buddhism and Sikhism.The main obstacles of cross-cultural adaptation are language,accommodation and diet.Economic status and stress of interpersonal relationship are also the main causes affecting the physical and mental health of medical international students.

BACKGROUND: Endothelial cell structural and functional integrity is importnat decisive fatcor for ischemic time-window and hemorragic transformation follwing brain ischemic injury.OBJECTIVE: To investiagte the endotheliocyt endurance to various course of ischemic injury basing on dynamical observation of morphological and ultrastructural changes of endotheliocyte during IR injury.DESIGN: Randomized controlled experiment.SETTING:Neurological Internal Department of the Second Affiliated Hospital of Jinan University.MATERIALS: This experiment was carried out in the Animal Experimental Laboratory of the Second Affiliated Hospital of Jinan University from March 1998 to March 1999. Totally 53 SD rats were randomly dihours of 6 rats.METHODS: Thread-bolt occlusion method was used to establish focal brain ischemia model on rats. Brain tissue was evenly cut into five coronary segments: namely A, B, C, D and E, segments C underwent TTC staining for marginal region location. segments D was taken for routine dehydration, transparency, envelop, slice and HE staining,optical microscopic observation. Ischemic surrounding area and central brain tissues was obtained from slice B, fixed and enveloped before cutting into ultrathin slices that was observed under transmission electron microscope.the occurring time of hemorrhagic infarction at different ischemic time cell vacuolization degree in foot process layer at different ischemic time points.RESULTS: Totally 53 rats were enrolled in this experiment and all data was entered into results analysis. Under optical microscope: Neuropil loose and small vascular surrounding edema was observed at ischemia 3 hours.Small arterial broken and hemorrhage occurred at ischemia 12 hours reperfusion 3 hours. Under electron microscope: Capillary endothelial nuclear swelling was observed at ischemia 3 hours, with cytoplasmic pinocytosi increasing and vacuolization in foot process layer appearing+; At ischemia 3 hours reperfusion 3 hours, the foot process layer vacuolization in center area was (++) and (+++) in marginal area; while at ischemia 6 hours reperfusion 3 hours, endothelia tight junction opened and vacuolization in foot process layer was (+++); pinocytosis was found obviously reduced after ischemia 12 hours reperfusion 3 hours, mitochondrial swelling was seldom observed, but tight junction increasingly opened and vacuolization in foot process layer appeared (+++) - (++++).CONCLUSION: Obvious structural changes of endotheliocyte appeared in post-ischemia 3 hours, endotheliocyte tight junction openning was observed at ischemia 6 hours, and hemorrage transformation occurred after ischemia 12 hours, mainly at the post-reperfusional ischemia center.

ObjectiveThe purpose of this study is to discuss the diagnostic accuracy of nornalized liver ADC using the spleen and renal cortex as reference organs for the diagnosis of liver fibrosis.Methods Forty three patients with liver disease (chronic liver disease group) at compensated stage and 10 healthy volunteers (control group) were retrospectively assessed with diffusion-weighted imaging at a 3.0 T MR unit.Liver ADC,spleen ADC,renal ADC and normalized ADC (defined as the ratio of liver ADC to spleen ADC or renal cortex ADC,S-ADC and R-ADC for short) were measured in patients stratified by fibrosis stage.Spearman analysis was used to see the correlation between fibrosis stages and ADC,one-way ANOVA was used to compare the ADCs in different fibrosis stages.Logistic regression analysis was used to determine the performance of ADC for prediction of liver fibrosis,and show the area under the curve ( AUC),sensitivity and specificity choosing the optimal cutoff value that maximized the Youden index.ResultsTen volunteers belonged to SO stage.From SO to S4 stage,there were 2,5,9,12 and 15 patients,correspondingly,liver ADCwere (1.37±0.13) ×10-3,(1.33±0.16) ×10 -3,(1.17±0.16) ×10-3,(1.23±0.14) ×10-3and ( 1.12 ±0.11 ) × 10-3mm2/s,S-ADC were 1.86 ±0.18,1.68 ±0.12,1.34 ±0.14,1.48 ±0.15 and 1.34±0.10,R-ADC were 0.71 ±0.08,0.68 ±0.12,0.61 ±0.09,0.64 ±0.11 and 0.60 ±0.08respectively,and the differences among them were significant ( F =6.48,18.70 and 3.04,P ＜0.05 ).The correlation between fibrosis stage and S-ADC was stronger than between fibrosis stage and liver ADC,R-ADC (r =- 0.71,- 0.51,- 0.41 ;P ＜ 0.01 ).S-ADC was superior to liver ADC and R-ADC for detection of S2,S3 and S4 fibrosis stage (Youden index:0.91,0.58,and 0.59).ConclusionSpleen normalized liver ADC improves diagnostic accuracy for detection of liver fibrosis than liver ADC and renal normalized liver ADC.

Objectives To provide prenatal diagnosis and genetic counseling for four athigh-risk pregnant women with a suspected family or personal history of fragile X syndrome (FXS) by genetic screening of fragile X mental retardation (FMR1) gene.Methods This study was conducted on four pregnant women (No.l to 4) who received outpatient treatment in Peking University First Hospital from August 2014 to June 2016.Genomic DNA was extracted from peripheral blood samples of the pregnant women and six of their family members,four of which were suspected or confirmed FXS and the other two were FMR1 gene carriers.Amplide X kits were used to detect CGG repeat size in FMR1 gene.Two amniocytes and one chorionic villi samples were collected from three pregnant women to extract DNAs for FMR1 gene and karyotyping analyses.Results There were patients diagnosed with FXS in all the families by detecting CGG repeat numbers in FMR1 gene.The pregnant woman No.1 was a permutation carrier;No.2 carried normal FMR1 alleles while her brother had a mutation with over 20 CGG repeats in FMRI gene at chromosome X.No.3 and 4 were full mutation carriers with over 200 CGG repeats in FMR1 gene.After genetic counseling,No.3 decided to terminate the pregnancy due to abnormal fetal karyotype (47,XY,+21) and full mutation of FMR1 alleles.No.1 and 4 continued to pregnancy as their fetuses were normal in FMR1 alleles and karyotype.No.2 continued to pregnancy as her fetus was free of FXS risk.Conclusions Prenatal diagnosis and genetic counseling should be conducted on women at highrisk for FXS to avoid birth defects.People with a family history of FXS should be tested for FMR1 gene carrier status.

AIM:To compare the difference between Shuxiong Tablet(Radix et Rhizoma notoginseng,Flos Carthami,Rhizoma Chuanxiong)(SXT) and its effective compounds group(SECG) in anti-inflammatory,relieving pain,anticoagulation,anti-thrombus and myocardial protection.METHODS:We adopted experiments of carrageenan-induced paw edema,hot water shrink trail,clotting time in mice,and thrombus in arteriovenous shut,and pituitrin induced acute myocardial ischemia in rats.RESULTS:Both of SXT and SECG could inhibit the tumefaction,decrease PGE_2 and SOD in inflammatory tissue;enhance the pain threshhold of mice;extend clotting time;inhibit the thrombosis;inhibit myocardial ischemia,significantly decrease CK,LDH,MDA and increase SOD in myocardial tissue.CONCLUSION:SXT and SECG have the same effects on anti-inflammatory,relieving pain,anticoagulation,anti-thrombus and myocardial protection.Further more,their dose-response relationship curves were similar,suggesting that SECG having the main effective components of SXT,could take place of SXT in clinical research.

Objective To investigate the feasibility and safety*with the low dose fentanyl for valve replacement fast track cardiac anesthesia. Methods 40 cases with cardiac valve replacement were divided into two groups,group Ⅰ :20 cases with fast track anesthesia,group Ⅱ :20 cases with large dose fentanyl anesthesia. The blood presure, HR, SpO2, CVP,Temperature, airway pressure were observed at different time points. The postoperative extubation time,ICU stay,hospital stay,postoperative pulmonary complications were recorded. Results The fentanyl(μg/kg) of group Ⅰ (23. 25 ± 1.32) was less than that of group Ⅱ ( 35.75 ± 6. 84) ( P < 0. 05 ); Extubation time (hours), ICU stay ( hours),length of stay (days) were ( 7.92 ± 3. 16), (25. 12 ± 7.50), ( 28.50 ± 8. 94 ) in group Ⅰ , and that were (13.70±5.63) ,(37.53 ±13. 19) ,(38.00 ± 12. 90) in group Ⅱ. The group Ⅰ were shorter than the group Ⅱ ,P<0. 05; There were four cases of lung infection in group Ⅱ, no one in group Ⅰ ( P < 0. 05 ); There were significant differences between the two groups. There were no significant differences in the remaining observations. Conclusion The low dose fentanyl anesthesia for fast track anesthesia in heart valve replacement surgery was reliable and safe;It could help to reduce postoperative extubation time, ICU stay time and hospital stay time and reduce postoperative pulmonary complications and hospital costs.