Objective To investigate the renal protection of Chinese cobra venoms (CCV) and its mechanism in renal ischemia/reperfusion (I/R). Methods Thirty-two rats were divided into four groups. 0.1% CCV was separately infused into abdominal cavity at 0.5 h, 24 h before reperfusion in group Ⅰ and Ⅱ . Group Ⅲ suffered from kidney I/R was served as pathological control. Group Ⅳ was sham operation group. BUN and Scr were measured before ischemia and 24 h after reperfusion. Complement C3 was observed at 0, 0.5, 2, 24 h after reperfusion. The kidney samples were examined by HE stain under light microscopy. Apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP-biotin in situ nick-end labeling(TUNEL). Results Significant histological damage, apoptosis of tubular cell and impaired renal function were found in group Ⅰ and Ⅲ.The above indexes decreased to a less extend in group Ⅱ (P

Objective To demonstrate the superiority and feasibility of using bioluminescent image to monitor the islet graft after islet transplantation.Methods Diabetic models were established by intraperitoneal injection of streptomycin into mature male C57BL/6 mice.Islets were harvested from the pancreas of C57BL/6 and Bclb/c mice by digestion and purification,and transfected with Lueiferase gene.The mouse diabetic models were divided into iso-transplantaion group (n=20) and allo-transplantation group (n=7).The islets of C57BL/6 were transplanted into iso-transplantaion mice with different doses (10,50,110 and 200,n=5 in every dose),and Bclb/c mouse islets were transplanted into allo-transplantation group.The islets were transplanted into the subcutaneous fat tis- sue near left scapula.The receptor mice were scanned with CCD camera to get bioluminescent images at different scheduled time points,and the changes in random blood glucose of allo-transplantation group were observed.Results On day 6 after transplantation,the scanning image showed that the pi- xel intensity from the region of interest (ROI) was increased with the increased number of islet grafts and they had a positive correlation.The random blood glucose was reduced to the normal level in the first 2 days,and then increased again to the diabetic level on 11 days averagely,while pixel intensity from the ROI reached the peak on day 6-7,and then reduced rapidly after islet transplantation in allo- transplantation group.The beginning of pixel intensity reduction occurred on day (6.14?0.90), while that of the random blood glucose raise occurred on day (10.00?0.82) after transplantation,and the former alteration occurred obviously earlier than the latter (P

Acupuncture Therapy ; Deglutition Disorders ; etiology ; therapy ; Humans ; Male ; Middle Aged ; Stroke ; complications

Objective:To examine the plasma soluble interleukin 2 receptor (sIL-2R)and the expression of IFN-γR in helper T (Th)cells in peripheral blood of patients with recurrent oral ulcer(ROU).Methods:The peripheral blood was obtained from 21 pa-tients in active stage of ROU and 21 healthy controls.Flow cytometry was used to detect the quantity of IFN-γR in CD +4 T cells,ELISA was used to detect the level of sIL-2R.Results:The expression of IFN-γR in CD +4 T cells and sIL-2R content in plasma of the patients with ROU were significantly higher than those in the healthy controls(P <0.05).Conclusion:Over expression of IFN-γR in CD +4 T cells and sIL-2R in peripheral blood may be related to the genesis of ROU.

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Breast ; pathology ; Breast Neoplasms ; drug therapy ; pathology ; Cyclophosphamide ; therapeutic use ; Diagnosis, Differential ; Doxorubicin ; therapeutic use ; Female ; Follow-Up Studies ; Humans ; Lymphoma, B-Cell ; drug therapy ; pathology ; Lymphoma, Large B-Cell, Diffuse ; drug therapy ; pathology ; Male ; Middle Aged ; Prednisone ; therapeutic use ; Sarcoma ; pathology ; Spleen ; pathology ; Splenic Neoplasms ; drug therapy ; pathology ; Vincristine ; therapeutic use

Objective To investigate the effect of combined treatment of p15 gene transfection with arsenic trioxide(As_2O_3)on proliferation and apoptosis of human squamous esophageal carcinoma cell line EC109.Methods Plasmid pcDNA3.1(+)-p15 was introdueted into EC109 cell hy the lipo- somes.As_2O_3(2?mol/L)was added to stable transfeeted cells for succedent experiments.The existence of exogenous p15 gene eDNA and the expression of P15 protein were assayed by PCR and Western blotting,respectively.The proliferation and apoptosis were measured by means of MTT,colony forma- tion assay,transmission electron microscopy,The cell cycle and population of apoptosis were measured by flow cytometry.Results After transfection,p15 gene mRNA and protein expressed in EC109 cells. Combined p15 transfection with As_O_3,the EC109 cell growth and colony formation were significantly decreased(P＜0.05,P＜0.01),compared with either p15 transfected group or treated with As_2O_3 group.After combining p15 transfection with As_2O_3 for 3 days,EC109 cell cycle was more arrested at G_1/S.The population of G_1 phase cells was significantly increased(P＜0.05),the population of S phase cells was significantly decreased(P＜0.05),and the population of the apoptosis cells was significantly increased(P＜0.01),compared with either p15 transfected group or treated with As_2O_3 group.More obvious apoptosis was found in the group with combined treatment of p15 gene transfection and As_2O_3 by transmission electron microscope.Conclusion p15 gene transfection combined with As_2O_3 show a signifi- cant effect on enhancing proliferation inhibition and could induce more apoptosis on EC109 cells in vitro.

Objective:To investigate the expression of LAIR-1 on Treg cells and TGF-βin the peripheral blood of patients with oral lichen planus(OLP).Methods:The peripheral blood was obtained from 22 OLP patients and 22 healthy controls.Flow cytometry was used to evaluate the proportion of LAIR-1 on CD4 +Foxp3 +Treg cells,and the level of TGF-βwas measured by ELISA.Results:In the peripheral blood of OLP patients,the proportion of LAIR-1 on CD4 +Foxp3 +Treg cells and TGF-βcontent were significantly higher than those of the healthy controls(P ＜0.05),The proportion of LAIR-1 on CD4 +Foxp3 +Treg cells was positively correlated with the level of TGF-β(r =0.43,P ＜0.05).Conclusion:The high expression of LAIR-1 on CD4 +Foxp3 +Treg cells and TGF-βin the pe-ripheral blood of OLP patients may be responsible for the pathogenesis of OLP.

0.05).The Cormack and Lehane laryngeal exposure grades obtained by the Macintosh laryngoscope with and without ELM were significantly different(Z=3.55 P0.05).Of all 33 pediatric patients,successful orotracheal intubation using the GSLV was completed by one attempt in 31 patients and by 2 attempts in 2 cases.The time required to achieve successful tracheal intubation was 20-51(30.0? 7.9) s.Conclusions GSLV is as useful as Macintosh laryngoscope for laryngeal exposure and orotracheal intubation in children.When the orotracheal intubation is done using the GSLV in children,the distal end of a styletted endotracheal tube should be bent anteriorly to an angle of 70-80 degrees and ELM is routinely used.

Growth year is one of the important factors for the quality of Polygala tenufolia. In this study, primary metabolites and secondary metabolites were compared in 1, 2 and 3 years old P. tenufolia cultivated in Shaanxi Heyang. The samples were subjected to ultra-high performance liquid chromatography (UPLC)-quadrupole time-of-flight mass spectrometry (Q-TOF MS) and nuclear magnetic resonance (NMR) analysis, and the obtained data were analyzed using principal component analysis (PCA) and other statistical analysis methods. In addition, content and correlation of different metabolites were also calculated. The results showed no significance between main component contents in 2 year-old and 3 year-old P. Tenufolia, but 1 year-old was statistically different. The contents of primary metabolites, such as fructose, sucrose, and choline increased as time goes on, while glycine and raffinose decreased. The contents of secondary metabolites, such as onjisaponin Fg, polygalasaponin XXVIII, polygalasaponin XXXII increased, while polygalaxanthone III and parts of oligosaccharide multi-ester including tenuifoliose A, tenuifoliose C, tenuifoliose C2 and tenuifoliose H decreased with the extension of the growth years. Growth years has important impact on the quality of P. tenuifolia and the existing growing years of commodity P. tenuifolia have its scientific evidence. This study supplied a new method for the quality evaluation of Chinese medicinal materials.
Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Metabolomics ; Oligosaccharides ; Plants, Medicinal ; chemistry ; Polygala ; chemistry ; Quality Control