2.MULTIPLE ENDOCRME NEOPLASIA-AN ANALYSIS OF 68 CASES
Wenjuan ZHANG ; Xiaoying FU ; Ping LU ; Shilin WEN
Chinese Journal of Postgraduates of Medicine 2001;24(7):19-21
Objective To improve diagnosis effectiveness for patients with multiple endocrine neoplasia(MEN).Methods 68 patients with MEN were reviewed in this paper.The diagnosis,clnical features and principle of treatment were discussed.Results (1)MEN-Ⅰ 22.05%,MEN-Ⅱ 36.86%,MEN-Ⅲ 8.82% MEN-Ⅰ\,Ⅱ 33.72%.(2)The cases in female were more than male and ten years earlier than male.(3)Endocrinophathies involred in order were thyroid,adrenal medulla,parathroid and islet.Conclusion It is important that more than one endocrinopathy be examined in doubtful cases.
3.Studies on Live Yeast Cell Derivative Induced by High Temperature and H_2O_2
Fu-Ping LU ; Hua YANG ; Yu WANG ; Lian-Xiang DU ;
Microbiology 1992;0(05):-
This study was based on live yeast cell derivative (LYCD), which was produced by live yeast cell stressed with high temperature and H 2O 2. The results showed that pretreating of low dose(37℃and 0.2mmol/L H 2O 2) could increase the content of GSH and the activity of SOD and CAT. These pretreatment could induce the resistance to lethal concentration of H 2O 2. LYCD was produced by yeast treated with 37℃ and 0.2mmol/L H 2O 2. And it was found that the survival of yeast treated with lethal concentration of H 2O 2 obviously increased, while LYCD was added in yeast culture. It indicated that LYCD could have resistance to oxidative condition.
5.Exploration of Case-based Teaching Method in English Teaching of Dermatology Postgraduate Students
Juan CHEN ; Ping FU ; Danqi DENG ; Zheng LU ; Xiaohong ZHOU
Journal of Kunming Medical University 2013;(11):136-138
Objective To explore the effects of case method teaching in the medical postgraduate student of dermatology English teaching. Methods The experiment group was taught by case method teaching, and the control group was taught by traditional teaching model. The quantitative research and questionnaire investigation were used to evaluate the teaching methods. Results (1) There were significant differences in professional English test scores of Dermatology between the two groups;(2) The questionnaire investigation revealed that the case method teaching have achieved a higher evaluation from students in stimulating their enthusiasm of learning English,cultivating their ability of using language and improving capability of resolving clinical problem. Conclusion Case method teaching could help students cultivate the abilities to interpersonal communication and cooperation, raise the sense of innovation and innovation capacity,strengthen the relation between skills and theoretical knowledge, and improve the ability of self-management, learn cooperation and information literacy. But we should improve the case-based teaching and evaluation methods,in order to improve the teaching method of case teaching quality.
6.Expressions of CD147 and EGFR protein and their relationship with metastasis and prognosis of gastric cancer
Ping ZHOU ; Yong FU ; Wenhua GAO ; Ning LU
Journal of International Oncology 2016;43(3):184-187
Objective To investigate the expressions of extracellular matrix metalloproteinase inducer molecule CD147 and epidermal growth factor receptor (EGFR) protein in gastric cancer and their relationship with the pathological behavior and prognosis.Methods Immunohistochemical staining was used to detect the expressions of CD147 and EGFR protein in 63 cases of gastric cancer and 40 cases of paracancerous normal tissues.The study was also combined with analysis of the pathological behavior and clinical follow-up survey of gastric cancer.Results The positive expression rates of CD147 and EGFR protein in gastric cancer were 77.8% (49/63) and 42.9% (27/63),respectively,which were higher than those in paracancerous normal tissues 25.0% and 0 (x2 =27.851,P =0.000;x2 =23.233,P =0.000).In gastric cancer,the expressions of CD147 and EGFR were closely related to the depth invasion (x2 =5.992,P =0.014;x2 =13.148,P =0.000),TNM stages (.x2 =8.481,P=0.004;x2=14.589,P=0.000),lymph node metastasis (x2 =5.270,P=0.022;x2=20.261,P =0.000) and prognosis of patients (x2 =5.606,P =0.018;x2 =4.982,P =0.026).The expression of CD147 was also significantly correlated with Ki-67 expression (x2 =10.481,P =0.001).CD147 expression was positively correlated with EGFR expression(r =0.309,P =0.014).Conclusion The expressions of CD147 and EGFR are closely related to carcinogenesis,metastasis and survival time of gastric cancer.Detecting the expressions of CD147 and EGFR may be prognostic indicators of gastric cancer.
8.Effect of long-term endogenous testosterone deprivation on function of voltage-dependent potassium channels in rat aortic artery
Ping ZHOU ; Lu FU ; Zhenwei PAN ; Dan MA ; Junxian CAO
Chinese Journal of Pathophysiology 1986;0(03):-
AIM:To explore the effect of long-term (6 months) endogenous testosterone deprivation by orchidectomy on the function of voltage-dependent potassium channels of vascular smooth muscle cells in rats. METHODS:Wistar rats were raised for 6 months after castration. Isometric tension measurement of aortic rings,whole-cell patch-clamp technique and Western blotting analysis were employed to examine the functional and posttranscriptional alterations of voltage-dependent potassium channels. RESULTS:Voltage-dependent potassium channel blocker,4-aminopyridine,significantly decreased the constriction of aortic artery rings from male rats after 6-month castration. In castrated rats the amplitude of voltage-dependent potassium currents of aortic artery smooth muscle cells was significantly decreased compared with that in control rats. Meanwhile,the expression of Kv 1.5 channel protein,which plays an essential role in mediating vasomotor function,was also reduced. The functional and molecular alterations of voltage-dependent potassium channels were both restored when the rats were concomitant applied with physiological level of testosterone after castration. CONCLUSION:Long-term deprivation of endogenous testosterone in rats significantly attenuates the function of voltage-dependent potassium channels,and the decreases in expression of Kv1.5 channel protein accounts for this alteration. Long-term application of physiological concentration of testosterone,which recovered the impaired function of voltage-dependent channels,may be beneficial for male gender with hypotestosteronaemia.
9.Effect of Naoxueshu oral liquid on cerebral hemorrhage caused by hypertension
Ming LU ; Ru-ping XIE ; Yu FU ; Daozhen XIE
Chinese Journal of Rehabilitation Theory and Practice 2004;10(5):304-305
ObjectiveTo investigate the effect of Naoxueshu oral liquid on cerebral hemorrhage caused by hypertension. Methods140 patients with cerebral hemorrhage were randomly dividied into two groups with a 1∶1 ratio,treatment group and control group. Patients in treatment group received Naoxueshu oral liquid 30 ml daily,while that in control received Naoxuekang oral liquid 30 ml daily. The changes of score on Chinese medical integration,National Institutes of Health (NIH) Stroke scale and the volume of intracranial hemorrhage were evaluated before and a month after treatment.ResultsThe decrease of NIH score of patients in treatment group was (12.73±3.94),but(4.72±3.01) with the control's (t=13.5327,P<0.001). The decrease of the volume of intracranial hemorrhage of patients in treatment group was (13.28±4.17) ml,but (8.37±7.24) ml with the control's(t=4.9228,P<0.001). ConclusionComparing with Naoxuekang,Naoxueshu can accelerate absorption of hematome and ameliorate the recovery of neurological disability more efficiently.
10.Molecular mechanism for berberine to improve insulin resistance in 3T3-L1 adipocytes
Ping YI ; Fu-Er LU ; Guang CHEN ; Li-Jun XU ; Kai-Fu WANG ;
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
Objective To investigate the effect of berberine on insulin resistance induced by free fatty acid in 3T3-L1 adipocytes and the possible molecular mechanism.Methods 3T3-L1 adipocytes were treated with 0.5 mmol/L palmitic acid to induce insulin resistance.Berberine was used for treatment and aspirin for positive control.Glucose oxidase method was employed for measuring the glucose consumption in the medium and 2-deoxy- [~3H]-D-glucose method was used for the determination of glucose uptake.Western blot was used for the determination of IKB kinase(IKK)?SerlS1 phosphorylation,insulin receptor substrance-1(IRS-1)Ser307 phosphorylation,the protein expression of IKK?,IRS-1,phosphatidylinositol 3-kinase(PI-3K)p85 and glucose transporter 4(Glut4).Results After the treatment with 0. 5 mmol/L of palmitic acid for 24 h,glucose consumption by 3T3-L1 adipocytes was decreased by 41%,insulin-stimulated glucose transport was inhibited by 67%,IRS-1 and PI-3K p85 proteins were reduced, and phosphorylations of IKK?Ser181 and IRS-1 Ser307 were induced.The above results were reversed by adding berberine or aspirin.But Glut4 and IKK?protein abundance was not changed during this study.Conclusion Berberine significantly improves insulin resistance induced by free fatty acid in 3T3-L1 adipocytes via inhibiting IKK?serine phosphorylation.