Objective To compare effects of laparoscopic versus open anterior resection of rectal carcinoma on immune functions.Methods A total of 38 patients were given either laparoscopic(18 patients) or open(20 patients) anterior resection of rectal carcinoma from April 2004 to June 2005 in this department.The percentages of T-lymphocytes(CD_3,CD_4,and CD_8) and natural killer cells were calculated by using the flowcytometry.The levels of immunoglobins(IgG,IgA,and IgM) and complements(C_3 and C_4) were detected by using the immunonephelometry.Results In levels of T-lymphocytes,immunoglobins,and complements,there were no statistically significant differences between open and laparoscopic groups 24 and 96 hours after operation.In levels of natural killer cells,no differences were observed in laparoscopic group before and after operation(24 and 96 postoperative hours) while a significant decrease were found in open group after operation.Conclusions As compared with open surgery,laparoscopic anterior resection of rectal carcinoma has less influence on natural killer cells.

Objective To explore clinical effects of laparoscopy applied in the female patients with infertility. Methods 468 cases of female infertility patients were diagnosed and treated by laparoscopy and 168 cases received follow-up survey. Results Among those infertility patients, the patients of normal pelvic cavity and the abnormal one were 49(10.47% ) and 419( 89.53% ). Among those patients of abnormal pelvic cavity .fallopian tube disease in 276 cases(65.87% ) was at the first place. The constituent ratio of endometriosis,ovary tumor,polycystic ovary,uterine myoma,female genital tract malformation and pelvic tuberculosis was 14. 80% ,5. 97% ,4. 30% ,3. 82% , 3. 34% and 1.91% .respectively. The patients of primary infertility and the secondary ones were 208 and 260 cases. In secondary infertility patients 69.23% (180/260) of patients were caused due to fallopian tube diseases,and the constituent ratio was higher than that in primary infertility 46.15% (96/208) (x2 = 14.05 ,P <0.05),while in primary infertility,the constituent ratio of endometriosis and normal pelvic cavity were 20.19% ,15.38% ,which were higher than that in secondary infertility respectively(x2 = 11. 78,11. 16, all P < 0.05). The pregnancy rate of 168 cases followed-up was 45.24% (76/168).47.37% ,84.21% pregnancy occurred within 6,12 months with laparoscopy. The shortest and longest time of pregnancy was 2 and 30 months, respectively. The median was 8months. Conclusion Laparoscopy playd an important role in diagnosis of infertility and improvement of pregnancy rate.

OBJECTIVE: To investigate the relationships of electrophysiological characteristics between speech evoked auditory brainstem response (s-ABR) and auditory mismatch negativity (MMN), so as to provide more clues for the mechanism of speech cognitive behavior. METHOD: Thirty-three ears in 33 normal hearing adults were included in this study. Their s-ABR were recorded with speech syllables /da/ at 80 dB HL intensity. Meanwhile, two MMNs were recorded with 1 kHz frequency deviant extent and 40 dB intensity deviant extent in them. The electrophysiological characteristics of s-ABRs and MMNs, as well as the relationships of MMN latencies between s-ABR parameters including latencies in time domain, fundamental frequency(F0) and first formants(F1) in frequency domain were analyzed statistically. RESULT: MMN latency of frequency deviance showed a negative correlation tendency with s-ABR transient components, and it showed a positive trend with sustained components of s-ABR. While MMN latency of intensity deviance showed a positive correlation with s-ABR latency of peak V, A and D respectively, and it negatively showed a correlation with s-ABR latency of other peak s and amplitude of F0 and FI respectively. Only the s-ABR latency of peak F and MMN latency of frequency deviance, and the F0 amplitude of s-ABR and MMN latency of intensity deviance were moderate correlation statistically. CONCLUSION It was probably the neurons of frequency deviant MMN unmatched the characteristics of frequency with the neurons of s-ABR transient component, but well matched the characteristics of frequency with the neurons of s-ABR sustained component. Similarly, the neurons of intensity deviant MMN probably matched the characteristics of intensity with neurons of different components of s-ABR or not. These results may formed as a valuable clue for further investigation of speech perception and temporal processing abilities.
Acoustic Stimulation ; Adult ; Evoked Potentials, Auditory, Brain Stem ; Humans ; Speech ; Speech Perception

Objective To discuss the diagnostic value of the lumbar intervertebral disc degeneration by CT scanning.Methods The clinical representation and CT view of 809 cases with degenerative lumbar intervertebral disc were retrospectively analyzed.Results According to the different CT representation character,the degenerative lumbar intervertebral disc may be divided into the lumbar intervertebral disc denaturation bulge in 96 cases and the lumbar intervertebral disc herniation in 713 cases.Conclusion CT scanning is a value method to diagnosing degenerative lumbar intervertebral disc.

Objective To improve the knowledge of lumbar posterior marginal cartilagionous node.Methods CT findings of lumbar posterior marginal cartilaginous node in 52 cases were analyzed.Results Three CT characters were:summarized cartilagionous node,centrum posterior marginal osseous absence and protruding follicle ring fragment.Conclusion CT scanning hes defiuite diagnositic value for the lumbar posterior marginal cartilaginous node.

Objective To improve recognition of the processus articularis arthropathy.Methods CT findings of the processus articularis arthropathy was analysed.Results According to the CT findings of the 94 cases processus articularis arthropathy,CT signs and the syndrome of this disease were summarized five types:①zygapophyseal hypertrophy and osteophyte formation;②stricture or asymmetry of articular space ;③vacuum phenomenon of articular space ;④pachynsis or calcification of articular capsule ;⑤articular instability.Conclusion The processus articularis arthropathy is a very high incidence of a disease.The CT scanning is very important significance to the disease.

Atrioventricular Block ; complications ; Child ; Electromyography ; instrumentation ; methods ; Female ; Histiocytic Necrotizing Lymphadenitis ; complications ; Humans ; Research Report ; Thrombocytopenia ; complications

Objective:To compare the time domain and the frequency domain of speech-evoked auditory brainstem response measured by stimulation of left and right ears, and to explore the difference and possible reasons of neural coding for speech from different ears in auditory brainstem.Method:Speech-ABRs to syllable /da/ of 31 healthy adults were recorded. Statistical analysis was performed on time-domain parameters, such as latencies and amplitudes of featured peaks, and frequency-domain ones, such as amplitudes of the fundamental frequency and the first formant of speech-ABRs ranging from 20-50 ms. A scoring criterion to grade the appearance of featured waves was proposed for waveform evaluation.Result:There were no significant difference for the latencies of binaural featured peaks and amplitudes of feature peaks(except peaks A and O). The waveform scores of right ear were greater than that of left ear. The amplitudes of fundamental frequency of binaural waves were both greater than that of the first formant. There was no significant difference of amplitudes of fundamental frequency and the first formant between two ears.Conclusion:The origins and distributions of speech-ABR are essentially symmetrical in brainstem in contrast with the hemisphere asymmetry of speech.

Objective To explore the effect of miR-24-2 on the in vitro proliferation of U-2OS cells. Methods U-2OS cells were randomly allocat-ed into 4 groups:miR-24-2 mimic group,miR-24-2 inhibitor group,negative control group,and normal control group. MicroRNAs were transfected into U-2OS cells using Lipofectamine?2000. miR-24-2 expression level and the proliferation of U-2OS cells after transfection were detected by real-time quantitative RT-PCR(RT-qPCR)and MTT proliferation assays,respectively. Results RT-qPCR results showed that miR-24-2 level was sig-nificantly higher in the miR-24-2 mimic group and lower in the miR-24-2 inhibitor group than those in the controls,indicating the successive trans-fection. MTT proliferation assay results proved that the cell viability was significantly lower in the U-2OS cells transfected with miR-24-2 mimic and higher in inhibitor groups compared to the control. Conclusion MiR-24-2 inhibits growth of the U-2OS cells,which could be a potential biomarker in the treatment of osteosarcoma.

Objective To elucidate the molecular basis on the differences of infectivity in infected cells between Sindbis virus(SINV:YN87448 virus)and Sindbis like virus(SINLV:XJ-160 virus).Methods Compare the E(glycoprotein)gene sequence and secondary structure of YN87448 virus and XJ-160 virus by bioinformatics analysis.Analyze the contribution of E gene to the biological differences between SINV and SINLV by constructing recombinant virus.Results By bioinformatics analysis,YN87448 virus and XJ-160 virus have the same genomic structure,which has 11 717 nt and 11 626 nt respectively.There are 82 amino acid differences between E gene of these two viruses,and showed scattered distribution.The main peak is basically the same for the hydrophobic of the E gene protein,but in some region existing small differences.The recombinant virus which exchanged the E gene of XJ-160 virus with YN87448 virus totally showed the biological character of YN87448 virus,either in the showing time of CPE,plaque forming time and plaque diameter,or in expression of functional proteins.Conclusion E gene plays a major role in the differences of infectivity in infected cells between SINV and SINLV,this result provide the molecular biological evidences for elucidating the biological differences between SINV and SINLV.