Objective This paper is mainly to discuss accuracy and clinical application value of MDCT double-period enhanced scanning with low -tension water enteroclysis for colon cancer preoperative TNM stag-ing.Methods Sixty-two colon cancer patients with complete images and pathological data were selected in our hospital from January 2012 to May 2013 .We retrospectively analyzed CT image changes of the tumor location ,the extent of tumor invasion,the surrounding fat space,lymph node metastasis and distant metastasis.We compared them with postoperative pathology to prove the accuracy of MDCT double -period enhanced scanning with low -tension water enteroclysis.Results The results showed that its accuracy rate reached to 90.32%(56/62)in co-lon cancer preoperative Stage T,80.64%(50/62)in Stage N,and 100%(62/62)in Stage M respectively.Con-clusions MDCT double-period enhanced scanning with low -tension water enteroclysis can accurately display the site of colon cancer and determine the scope of tumor invasion ,lymph node metastasis and distant metastasis , and give more precise diagnosis of colon cancer and preoperative staging assessments .In conclusion , it can be used as the preferable method of preoperative examination in the colon cancer .

Pulsed field gel electrophoresis(PFGE)is a new type technique of gel electrophoresis which can be used to separate large DNA molecules.It has been widely applied to the karyotype analysis,identification of species groups,genetic orientation and genetic analysis for fungi.This article describes the principle,development and general manipulative procedure of PFGE,and elaborates the application in the molecular research of fungi.

OBJECTIVE:To explore the spasmolytic effect of baked Radix Vladimiriae on in vitro intestinal muscle in rabbits. METHODS:Magnus in vitro intestinal muscle accumulative dose method was adopted to observe the dose-effect relationship of petroleum ether parts of baked Radix Vladimiriae with in vitro intestinal muscle of rabbits and its impact on the spasm of rabbit intestinal muscle induced by acetylcholine, histamine phosphate and Bacl2. RESULTS:There was a certain dose-effect relationship between petroleum ether parts of baked Radix Vladimiriae and spasmolytic mechanism of rabbit in vitro intestine. It showed the effect of acetylcholine, histamine phosphate and Bacl2. CONCLUSION:The inhibition effect of petroleum ether parts of baked Radix Vladimiriae on normal in vitro intestinal muscle increase as long as dose increase. The inhibition mechanism may be associated with acetylcholine competing with histamine to block M receptor and H1 receptor as well as to inhibit excitability of intestinal muscle.

Objective To discuss the clinical influence and advantages of applying the local flap of finger tip lateral skin to repair the fingertip defective injuries.Methods From August,2013 to December,2015,used the way of local the flap of finger tip lateral skin to repair the first-fourth fingertip defects for 32 cases and 32 fingers,among which there were 20 males and 12 females;finger categories:11 thumbs,9 index fingers,8 middle fingers and 4 ring fingers.The defective area of fingertips was 0.5 cm×0.8 cm-1.0 cm×1.5 cm,all with bone exposure,23 cases had defects at the end part of phalanx,32 cases had different extent of nail bed defects.Reasons for the injuries:13 cases of grind injuries,11 cases of punch pressing injuries,8 cases of sharp tool cutting injuries.The time from injury to the treatment was 0.5-3.0 h,all of them had no associated injuries.Results Thirty-two cases of finger flaps all survived,with the follow-up for 3-24 months and average follow-up of 6 months,the flaps were slightly bloated after 3 months comparing with the normal skin,there was no contracture in the flap area and pedicle scars,two-point discrimination was 4-5mm,the farinterphalangeal joint flexion was normal.Conclusion The local flap of fingertip lateral skin can repair the defects of fingertip with less scar,not bloated flap,satisfying appearance,operation is simple,and good function,so it's the ideal way to repair the defects of fingertip.

Objective To compare the effects of two head holders on the setup errors during head and neck neoplasms radiotherapy.Methods Totally 30 patients were divided into groups A and B randomly and equally.Group A applied TypeSTM Extension for setup,and group B used Type-S Overlay Board,with thermoplastic film applied to fixation.Varian23IX image guided linear accelerator was involved in the therapy,and the patients underwent CBCT-guided therapy once a week in 6 weeks.The setup errors at left-right,head-foot and anterior-posterior directions were obtained by bone registration and manual checking.Results The absolute setup error at anterior-posterior direction of group A,was (2.31 ± 1.35) mm,which was significantly higher than (1.88±1.79) mm of group B (P=0.032).There were no obvious differences between the setup errors of group A and those of group B (P＞0.05),and the weekly absolute errors of group A were not statistically different from those of group B (P＞0.05).Conclusion There are no significant differences between the effects of the two head holders on the setup errors during head and neck neoplasms radiotherapy,and proper head holder can be selected according to clinical requirements.

Background Graft rejection is a primary cause of corneal transplantation failure,especially in high-risk keratoplasty.How to extend the survival time of graft is a problem to be solved.Objective This study was to investigate the influence of immune tolerance on high-risk rat keratoplasty induced by donor bone marrowderived immature dendritic cells (imDCs) transfected by chemokine receptor 7 (CCR7) recombinant adenovirus (Ad).Methods Bone marrow-derived imDCs were isolated and cultured from femur marrow of one male Wistar donor rat.The cells were transfected using recombinant Ad vector with rat CCR7 gene and resuspended in 500 μl PBS containing 1％ fetal bovine serum with the cells 1 × 107.High-risk corneal transplantaion models were established using monolateral corneal alkali-burn method in 60 SD rat recipients, and then allograft keratoplasty was performed with the 30 Wistar rats as donors.The models were randomized into the PBS group,imDCs group,imDCs with blank Ad vector group and imDCs with Ad-CCR7 group following the corresponding solution injection via caudal vein on preoperative day 7 and postoperative day 3 respectively.The survival time of graft was evaluated under the slit lamp microscope once per day.On the 14th day after operation, corneal sections were prepared from 6 eyes of each group for the pathological examination,and the relative expression levels of T helper cell 1 (Th1)-related factors,interleukin-2 (IL-2) mRNA and interferon-γ(IFN-γ) mRNA,as well as Th2-related factors, IL-4 mRNA and IL-10 mRNA, were detected by reverse transcription PCR (RT-PCR).The use and care of animals complied with the ARVO statement Results The mean survival time of grafts was (10.44±1.88) , (16.00±2.18) , (15.11±2.03) and(23.67±2.83) days in the PBS group,imDCs group,imDCs with blank Ad group and imDCs with Ad-CCR7 group, respectively, with a significant difference among the 4 groups (F =53.005, P =0.000).Compared with the PBS group, the mean survival time of grafts was considerably extended in the imDCs group,imDCs with blank Ad group and imDCs with Ad-CCR7 group (t=5.220,4.385,12.423 ,all at P=0.000) ,and a remarkble prolongation of graft survival duration was seen in the imDCs with Ad-CCR7 group in comparison with the imDCs group and the imDCs with blank Ad group (t =7.204,8.039,both at P=0.000).The relative expression levels of IFN-γ mRNA and IL-2 mRNA in the grafts were significantly lower,but the relative expression levels of IL-4 mRNA and IL-10 mRNA were significantly higher in the imDCs group,imDCs with blank Ad group and the imDCs with Ad-CCR7 group than those in the PBS group (all at P＜0.05).Compared with the imDCs group and the imDCs with blank Ad group, the relative expression levels of IFN-γ mRNA and IL-2 mRNA in the grafts were remarkably delined,but the relative expression levels of IL-4 mRNA and IL-10 mRNA were remarkably elevated in the imDCs with Ad-CCR7 group (all at P =0.000).Conclusions Application of imDCs transfected with CCR7 recombinant Ad via vena caudalis can prolong the survival time of grafts after keratoplasty of SD rats probably by affecting the balance of Th1/Th2 cytokines.

The hypoglycemic activity and its mechanism of Jatrorrhizine (Jat) were studied. The normal mice and alloxan-induced hyperglycemic mice were given with different doses of Jat. Blood glucose and liver glycogen levels were determined by spectrophotometry with glucose-oxidase and iodine reagents respectively. The levels of blood lactic acid (LC) and liver lactate dehydrogenase (LDH) activity were measured to explore the effect of Jat on anaerobic glycolysis. Succinate dehydrogenase (SDH) activity in liver was measured to evaluate the effect of Jat on aerobic glycolysis in liver. It was found that Jat (50 mg/kg, 100 mg/kg) could significantly decrease blood glucose level in a dose- and time-dependent manner in both normal and alloxan-diabetic mice, increase the activity of SDH, but had no significant effects on the LC level and LDH activity. Jat could significantly reduce the content of liver glycogen in normal mice. Moreover, Jat could inhibit the platelet aggregation in rabbits in vitro in a dose-effect relationship. It was concluded that Jat induced the pronounced decrease in blood glucose in normal and hyperglycemic mice. The hypoglycemic activity of Jat may be attributed to the enhancement of aerobic glycolysis.

Animals ; Lung ; immunology ; pathology ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; metabolism ; physiopathology ; Spleen ; immunology ; pathology ; fas Receptor ; analysis

Objective To compare the effects of robot-assisted gait training with supported treadmill training (BWSTT) for hemiplegic patients after stroke.Methods A randomized,single-blind,controlled study was performed.Forty-eight hemiplegic patients were divided randomly into a BWSTT group and a robot group with 24 in each Both groups received routine rehabilitation training.The robot group also received robot-assisted gait training,while the BWSTT group received treadmill training in which their body weight was supported.Both groups trained 20-30 minutes daily,5 days a week for 8 weeks in addition to their normal rehabilitation sessions.Their lower extremity functions and walking ability were assessed with the simplified Fugl-Meyer assessment (FMA) and using functional ambulation categories (FACs).Average step length,percentage of loading time on each foot and 3 min walking distance were tested using a Biodex Gait Trainer.All the assessments were administered before and 8 weeks after treatment.Results Both groups showed significant improvements in terms of the FMA,FACs,average step length,loading time on each foot and 3 min walking distance.The robot group,on average,showed significantly better improvements in average step length,percentage of loading time on each foot and 3 min walking distance than the BWSTT group.There was no significant difference in lower extremity function or walking ability in terms of the FMA or FACs.Conclusions Robot-assisted training gives better results than BWSTT in terms of improving average step length,equalizing the loading time on each foot and 3 min walking distance.

Objective To investigate the antimicrobial susceptibility and associated virulence genes of the Staphylococcus aureus?strains?isolated?from?blood,?cerebrospinal?fluid?and?other?sterile?body?fluids?in?Huashan?Hospital?from?year?1999?to?2014.?Methods MIC values of vancomycin and other antibiotics against S. aureus were measured by agar dilution method. Resistant genes mecA and mecC and virulence genes PVL and sasX were detected by PCR in the S. aureus strains. Results The overall prevalence of MRSA in S. aureus was 54.3 % (140 / 258) and 45.7 % (118 / 258) for MSSA. Resistance rates of MRSA to most antimicrobial agents were higher than MSSA. MSSA strains were still sensitive to all the antibiotics tested, resistance rate not higher than 11% except penicillin. MIC90?values?of?β-lactam?antibiotics?(except?penicillin)?to?MSSA?were?lower?than?1?mg?/?L.?No? staphylococcal strains were found resistant to vancomycin, teicoplanin or linezolid. The mecA gene was present in all the 90 MRSA strains, and sasX gene in 46.7% of the strains. The prevalence?of?MRSA?isolated?from?blood,?cerebrospinal?fluid?and?other?sterile?body?fluids?decreased?year?by?year.?No?mecC gene?or?PVL?gene?was?identified?in?these?MRSA?strains.?Both?sasX-positive MRSA and sasX-negative MRSA were resistant to?β-lactam?antibiotics,?fosfomycin?and?levofloxacin.?The?sasX-positive MRSA strains showed higher resistance rates to amikacin and trimethoprim-sulfamethoxazole than sasX-negative MRSA. Conclusions? The?MRSA?strains?isolated?from?blood,?cerebrospinal?fluid?and?other?sterile?body?fluids?in?Huashan?Hospital?were?resistant to most commonly used anbiotics. MRSA surveillance is critical for rational use of antimicrobial agents.