OBJECTIVE:To prepare temperature sensitive hydrogel which is of strength.METHODS:The hygrogel was prepared using CS,PVA and GP as matrix with concentration of CS(A),weight ratio of CS to PVA(B) and pH value(C) as factors and with initial gelatination temperature,strength and dehydration as indexes.The formula was optimized by orthogonal experiment and verified.RESULTS:The optimal formula was as follows:A 20 mg?mL-1,B 1 :1,C 7.2.The prepared hydrogel was fluid at 4 ℃ or room temperature while gelatinized at 37 ℃ within 10 min with strength of about 1.4 kPa.Gelatination time reduced along with the increase of temperature.The pH value had hardly changed during degradation in vitro within 28 d.CONCLUSION:The CS/PVA/GP hydrogel is simple and practical in preparation technique and is temperature sensitive and of strength.

· AIM: To study the expression of basic fibroblast growth factor (bFGF) receptor protein in human lens epithelial cells (HLECs). METHODS: Immunohistochemistry was used to detect the level of bFGF receptor protein and image analysis was adopted to perform the relative quantitative analysis on it. · RESULTS: There was bFGF receptor protein in HLECs accordingl to both qualitative and quantitative analysis. · CONCLUSION: bFGF receptor protein exists in HLECs and it is the material foundation for bFGF to improve the proliferation of HLECs.

Budd-Chiari Syndrome ; Child ; Humans ; Infant ; Male

Adolescent ; Child ; Child, Preschool ; Female ; Hepatocyte Growth Factor ; metabolism ; Humans ; Kidney ; metabolism ; pathology ; Male ; Nephrotic Syndrome ; metabolism ; pathology ; Proto-Oncogene Proteins c-met ; metabolism

Objective To know the currently prevalent genotype of rotavirus strains in Ningbo City,which will help to the study of vaccine of group A rotavirus.Methods The VP7 fragments were amplified by RT-PCR and sequence analysis was conducted from 2 of the 11 virus strains.Results VP7 amino acid sequences homogeneity of the two strains Ningbo 06-1 and Ningbo 06-3 was 90.2%.They all shared higher amino acid sequences homogeneity with G1 strain Wa(95.1% and 87.7%,respectively)than with other serotypes(G types)rotavirus(

[Aim] Discuss Chen's treatment effect of infants viral myocarditis ayyhythmia. [Methods] He made suit medicine on two cases and analysed the disease'classification and main clinical manifestation and relevant therapy and his empirical formula, according to some factors ,such as the etiology and pathogens, course of disease and so on. [Results] (1)For viral myocarditis early disease caused by tachyarrhythmia, Mr. Chen advocated using the methods to treat which is clearing away heat and toxin and calming the pulse, the prescription used is Qingxinjiedu Decoction;however, those ones with il ness are offered a way of suppiementing qi and nourishing yin and warming the heart yang in order to remove pulse frequency, Chen used the modified YiQiShengMai Decoction for treatment.(2) The professor Chen paid attention to clearing hot and humid and supplementing qi and warming yang to cure slow arrhythmia due to acute viral myocarditis,the modified Huolian Decoction is used for this disease; For the people with a long il ness delayed healing, Chen worked a Decoction by himself which is Wenyangfumai Decoction,so that it could supplement qi and warm yang and invigorate the circulation of blood through pulse; For the slow arrhythmia appeared to sequela period, Chen thought that treatment should be insisted on promoting blood circulation to remove blood stasis and warming and activating the heart yang, the prescription used is Tongmai oyal liquid.[Conclusion]Professor Chen had gained a satisfied effect to treat infants viral myocarditis arrhythmia with the methods of clearing away heat and toxin, supplementing qi and nourishing yin and warming the heart yang and removing pulse frequency from the different stages and types.

Objective To study the clinical and laboratory features of the patients with immunorelated pancytopenia(IRP).Methods The risk factors,manifestations,blood cell counts,bone marrow phenotypes,autoantibodies and immunosuppressive therapy response of 72 patients with IRP were analyzed.Then they were followed up for(6 ~36)months,to see their long-term outcome and the prognostic factors.Results The infection,anaphylaxis and pregnancy were highly suspected to be the risk factors of IRP.Most of these patients were with pancytopenia72.2%(52/72);91.7%(66/72)of them were anemic with large or normal MCV;75.0%(54/72)of them were with leukopenia;18.1%(13/72)of them had fever.Thrombocytopenia was common,but serious bleeding rare.68.1% of these cases were with normal or decreased bone marrow cellularities and increased normoblasts.They were all found to have positive results of bone marrow mononuclear cell Coombs test,negative results of routine hemolysis tests and no evidence of malignant clonal hematopoiesis.42.9%(18/42)of these patients had C3 decreased,and 19.0%(8/42)C4 decreased.Immunosuppressive therapy was administered to 72 IRP patients.The response rate at 36 months was 87.5%(28/32).Conclusion IRP is a blood disease induced by acquired autoantibodies mediated bone marrow inhibition or destruction.It manifests pancytopenia with relative higher percentages of reticulocytes and neutrophils,and responds well to immunosuppressive therapy.

Objective To evaluate the surgical treatment and CT diagnosis of retroperitoneal paraganglioma.Methods Clinical data,CT image and surgical treatment of 17 cases diagnosed as retroperitoneal paraganglioma were analyzed retrospectively.Results In CT scan,tumors were singular and located around the abdominal aorta and inferior vena cava in 13 cases,while in the other 4 cases tumors were located in the lower abdomen and pelvic cavity.The masses were oval shaped,varied in size and the margins were clear.Most mass had integral capsule.The density were uneven and the low density area didn't have enhancement in contrast enhancement CT.All of the 17 patients received complete tumor resection in which 13 cases were pathologically diagnosed as benign,4 cases was boundary or low malignant and 3 cases received second operation for recurrence.Conclusion CT is helpful in diagnosis and locating retroperitoneal paraganglioma.Surgical resection is effective and satisfactory.

ObjectiveTo investigate and evaluate the depression in patients with type 2 diabetes.Methods62 patients with type 2 diabetes (30 having complications, 32 not having complications) and 20 normal controls were assessed by Hamilton Depression Rating Scale (HRSD) of Peking University PES Mental State Application System.ResultsThe HRSD total scores of diabetics were higher than that of normal controls, and scores of diabetics with complications were higher than that without complications (P<0.05). The scores of anxiety/somatization and cognitive disturbance of diabetics were all higher than that of normal control (P<0.05).The scores of sluggish, dyssomnia of diabetics with complications were higher than that of normal controls (P<0.05), but there was no difference between diabetics without complications and normal controls (P>0.05). The scores of changing at day and night, weight, and the despair feel of diabetics were not obviously different with that of normal controls (P>0.05).ConclusionThe diabetics are often companioned with depression, and more severe when having complications.

AIM:To study the differences of basic fibroblast growth factor(bFGF)gene expression in lens epithelial cells (LECs) between fetuses and cataract patients. METHODS: In situ hybridization was used to detect bFGF mRNA in the LECs that were cultured and in tissue sections from fetuses and in the LECs from the anterior capsule of cataract patients. Image analysis was used for the relative quantitative analysis of bFGF mRNA. RESULTS: bFGF gene existed in the LECs that were cultured and in tissue sections from fetuses and in the LECs from the anterior capsule of cataract patients. The integral absorbance for the fetal cultured cells, the fetal tissue sections and the capsule membrane cells of cataract patients were 627.1±268.7, 131.5±42.8 and 79.2±26.3 respectively. The integral absorbance of fetal cultured LECs was significantly higher than that of fetal section LECs (P<0.01). The integral absorbance of cataract LECs was significantly lower than that of fetal LECs (P<0.01). CONCLUSION: The in vitro culture of LECs can improve bFGF gene expression. The bFGF gene expression in fetal LECs is significantly higher than that in cataract LECs.