1.Bioreactor in bioartificial liver system
Chinese Journal of Tissue Engineering Research 2010;14(3):545-548
OBJECTIVE: To introduce the basic functions, working conditions and requirements of bioreactot, additional, to discuss the research process of bioreactor.METHODS: PubMed database (http://www.ncbi.nlm.nih.) and CNKI database (www.cnki.nat/index.htm) were retrieved by the first author with search terms of "bioartificial liver, bioreactor, membrane material" both in English and Chinese. The time was limited between January 1994 and August 2009. Inclusion criteria: ①literatures closely linked to the article; ②papers published in more authoritative journals in recent years. ③the contents of old or duplicate documents were excluded.RESULTS: Totally 150 documents were seized by primary screen, 88 irrelative papers and 32 duplicate documents were excluded, finally, 30 literature entered further analysis. Bioartificial liver system has become an effective in vitro supportive treatment for hepatic failure patients. Bioreactor is an important ingredient of bioartificial liver, which provided a platform for the hepatocytes growth metabolism, substances exchange and immune isolation. The main cellulose semipermeable bioreactor comprises fiat membrane bioreactor and hollow fiber bioreactor, and the main types of semipermeable membranes contain mixed cellulose ester membrane, cellulose acetate membrane, cuprophan membrane, PVDF membrane, as well as poly(ethylene terephthalate) membrane.CONCLUSION: As a dynamic system, the optimal of control system in bioreactor is conductive to regulating mass transfer and establishing bionic physical or chemical gradients, which can implement the construction of hepatocyte with smallest unit. The selection of membrane is the key link in bioreactor construction of bioartificial liver system
2.Transport of melanocytes by chitosan membrane: an animal experiment
Dong DONG ; Siyuan XU ; Lifang FU ; Tao WANG ; Aie XU
Chinese Journal of Dermatology 2011;44(4):249-252
Objective To study the feasibility of using chitosan membrane to carry and transport melanocytes, in order to refine the technique for melanocyte transplantation with chitosan membrane. Methods Melanocytes were inoculated onto chitosan membrane and cultured for a period of time, then, electron microscopy,MTT assay and NaOH assay were carried out to estimate the adherence, growth and melanogenesis of the melanocytes. Skin wound surface was prepared in 12 nude mice, which were equally divided into 3 groups, test group inoculated with melanocytes on chitosan membrane, negative control group I treated with chitosan membrane without melanocytes, and negative control group II directly dressed immediately after the preparation of wound surface. On day 10 and 20 after the transplantation, confocal laser microscopy and immunohistochemistry were performed to observe the migration of melanocytes into the skin wound surface. Results Scanning electron microscopy and inverted microscopy showed that melanocytes were evenly distributed on and adhered well to the underlying chitosan membrane. As the growth curve of melanocytes demonstrated, chitosan membrane could support the normal growth of melanocytes, and no significant difference was observed in the synthesized melanin content between melanocytes cultured on the chitosan membrane and those in culture disks (0.087 ± 0.027 vs. 0.101 ± 0.036, t = 0.79, P > 0.05). Melanocytes were seen at the transplantation sites by confocal laser microscopy, and biopsy specimens from the transplantation sites stained positive for antimelan-A monoclonal antibody. Conclusions Melanocytes can adhere to and grow on the chitosan membrane,which can facilitate the migration of melanocytes to the transplantation sites in animals with the maintenance of biological activity of melanocytes.
3.Analysis on nosocomial infection of Corynebacterium.
Dong-ke CHEN ; Hong-tao XU ; Fu-pin HU
Chinese Journal of Epidemiology 2013;34(9):947-948
4.Comparison of naveibine and cisplatin versus gemcitabine and cisplatin in refractory metastatic breast cancer
Zhong-Fu SHAO ; Gang XU ; Ming ZHOU ; Yong-Dong CHEN ;
Cancer Research and Clinic 1999;0(05):-
0.05).The degreeⅢ~Ⅳthrombocytopenia was more common in group A than in group B,but the degreeⅢ~Ⅳhypolekocytosis and phlebitis was more serious in group B.Conclusion NC and GC for treating refractory metastatic breast cancer have a high response rate and tolerable side effects.
5.Prevention effect of 5-fluorouracil natural delayed-release microballoons on proliferative vitreoretinopathy
Xiao, CHEN ; Shuai, QIN ; Xu-dong, FU ; Qin, DING
Chinese Journal of Experimental Ophthalmology 2011;29(6):501-504
Background Proliferative vitreoretinopathy(PVR) is a tissue repair prevention and treatment of PVR in clinic.Natural delayed release microballoons are therefore becoming a hot spot for its easy manipulation,large lading dose and long acting duration.Objective This study was to evaluate the effect of 5-fluorouracil natural delayed release microballoons on the prevention of PVR.Methods The lymphocytes were collected from clean pigment rabbit to prepare the 8×107/ml cell suspension with complete culture fluid.PVR models were established in 45 healthy pigment rabbits by intravitreal injection of lymphocyte suspension.The animals were randomly divided into 3 groups and 15 rabbits for each.0.1ml normal saline,10g/L or 20g/L 5-fluorouracil natural delayed release microballoons were injected into vitreous cavity respectively.PVR was graded on Fastenberg's method under the slit lamp in 1,2,4,8 weeks.The animals were sacrificed and retinas were obtained for the histopathological and ultrastructural examination in the eighth week after administration of drug.Results The numbers of eyes with different grades of PVR were significantly different among 3 groups in 1 week,2,4,8 weeks(P<0.05).The eye numbers with PVR was significant less in 20g/L Fu group than those of 10g/L Fu group and normal saline group(P<0.05).There was statistical difference in PVR ranking among these 3 groups in 8 weeks after injection of drug(H=46.795,P<0.05).The morphology and ultrastructure of retinas under the light microscope and transmission electron microscope were near normal in all of the three groups.Conclusion Implantation of 5-fluorouracil natural delayed release microballoons into vitreous cavity is effective and safe in preventing PVR in experimental model,and the therapeutic effect of microballoons with 20g/L 5-Fu is better.
6.Rapid europium-sensitized fluorescent determination of ulifloxacin,the active metabolite of prulifloxacin,in human serum and urine
Peng DONG ; Na XU ; Bo FU ; Lei WANG
Journal of Pharmaceutical Analysis 2011;01(1):46-50
A new fluorescent method was developed based on the ulifloxacin-europium(Ⅲ)-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium(Ⅲ)and significantly enhanced the characteristic fluorescence of europium(Ⅲ).The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0×10^-8-2.0×10^-6M with a detection limit of 2.0×10^-10 M(3σ).This method is rapid and sensitive,and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.
7. Evaluation of in Vitro Release Profile and Local Retention of Estradiol Vaginal Thermosensitive Gel
Chinese Pharmaceutical Journal 2017;52(10):852-856
OBJECTIVE: To examine the in vitro release profile and in vivo retention of estradiol vaginal thermosensitive gel (E2-VTG). METHODS: E2-VTISG was prepared by cold dissolving method.The dynamic membrane dialysis method and HPLC-fluorometric method were used to determine the in vitro release characteristic of the estradiol vaginal thermosensitive gel.CRi Maestro was applied to evaluate the retention of E2-VTG in ICR mice with IR820 as the fluorescent marker. RESULTS: Estradiol could be released slowly from the thermosensitive gel and the release profile was fitted with Higuchi equation. It was speculated that estradiol was mainly released through diffusion.NIR imaging and fluorescence quantitative analysis showed that thermosensitive gel could reside in vagina for at least 8 h. CONCLUSION: Estradiol thermosensitive gel can prolong the drug residence time in vagina and sustain the drug release rate.
8.Dynamic Expression of HoxB5 in Lung Tissue of Neonatal Rats with Hyperoxia-Induced Chronic Lung Disease and Its Significance
wei, XU ; jian-hua, FU ; xin-dong, XUE
Journal of Applied Clinical Pediatrics 1994;0(04):-
0.05),and the expression of HoxB5 in the model group tapered after the 7th day,but the expression of HoxB5 increased and reached the peak on the 7th day and expressed in a stable level in the control group and were significantly higher than those of model group(Pa
9.Rapid europium-sensitized fluorescent determination of ulifloxacin, the active metabolite of prulifloxacin, in human serum and urine
Peng DONG ; Na XU ; Bo FU ; Lei WANG
Journal of Pharmaceutical Analysis 2011;01(1):46-50
A new fluorescent method was developed based on the ulifloxacin-europium (Ⅲ)-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium (Ⅲ) and significantly enhanced the characteristic fluorescence of europium (Ⅲ).The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0× 10 8 -2.0× 10-6M with a detection limit of 2.0× 10-10 M (3σ).This method is rapid and sensitive,and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.
10.Method for obtaining prior information of electrical impedance tomography
Canhua XU ; Xiuzhen DONG ; Cheng ZHEN ; Feng FU ; Wanjun SHUAI
Chinese Medical Equipment Journal 1989;0(01):-
The reverse problem of Electrical impedance tomography(EIT) is a highly ill-posed problem.It is concluded that spatial prior information could improve the final image quality.This paper proposes a new method for obtaining prior information.By this method,the inspected cross-section contour and internal structure for EIT can be achieved.