Objective To study the protective effect of FGP on liver damage in mice caused by carbon tetrachloride(CCl4).Methods Carbon tetrachloride was used to make the model of chemical liver damage.The mice were randomly divided into 5 groups,10 in each:FGP(10.0 ml/kg)+CCl4,FGP(5.0 ml/kg)+CCl4,FGP(2.5 ml/kg)+CCl4,CCl4(0.12%,10 ml/kg)and the control group.The mice were treated with FGP and CCl4 by gavage and intraperitoneal injection.The biochemical and pathological examinations were conducted to observe the liver damage.Results Compared with CCl4 group,treated with FGP at 5.0 ml/kg could reduce the serum ALT activity significantly,the histopathological findings showed an obvious improvement.Conclusion FGP may have some protective effects on acute live damage caused by CCl4 in the mice.

Objective To study the role of immunoregulation in patients with Recurrent vulvovaginal candidiasis(RVVC) that evaluate the efficacy of application with combining clotrimazole and rhIFN-? therapy.Methods Patients were divided randomly into two groups :treatment group(60) and control group(60). The patients in treatment group were scheduled to receive clotrimazole therapy, 400mg,pv,qd,and add to rhIFN-? 1000000u im,biw; control group only using clotrimazole therapy.Results The curative rate betweet two groups had significant difference (68.33 (41/60) vs. 35.0(21/60). The efficiency rate in control group was significantly higher than treatment group( (56.67(34/60)vs 26.67(16/60)),In treatment group ,the serum levels of IL-18(pg/ml)(before treatment (111.71?18.45),after treatment (189.18?28.17))and TNF-? (pg/ml)(before treatment (52.66?18.75),after treatment (124.25?43.77))were significantly increased (P0.05). Conclusion The application with combining clotrimazole and rhIFN-? can significant ameliorate the curative rate of RVVC,by regulate immune fanction in the patients that were induced the produce of IL-18 and TNF-?. rhIFN-?can improve cell-mediated immunity in patients with RVVC.

0.05);The rosettes looping rates of the2.5,5.0,10.0 mL/(kg?Bw)polypeptide groups were 33.3%,34.2%,39.6%higher than the control(injected with NS)(P

iple occurrence of the tumor, which can decline its recurrence and postpone its progression.

Adult ; Alkanesulfonic Acids ; adverse effects ; Humans ; Male ; Pemphigoid, Bullous ; chemically induced

The epitope-G1 gene of Bovine ephemeral fever virus (BEFV) glycoprotein was synthesised by PCR and cloned into expression vector pPIC9K to construct recombinant plasmid pPIC9K-G1. Then the pPIC9K-G1 was linearized and transformed into Pichia pastoris GS 115. The recombinant P. pastoris strains were selected by a G418 transformation screen and confirmed by PCR. After being induced with methanol, an expressed protein with 26 kDa molecular weight was obtained, which was much bigger than the predicted size (15.54 kDa). Deglycosylation analysis indicated the recombinant G1 was glycosylated. Western blot and ELISA tests, as well as rabbit immunization and specificity experiments indicated that the target protein had both higher reaction activity and higher immunocompetence and specificity. The recombinant G1 protein could be used as a coating antigen to develop an ELISA kit for bovine ephemeral fever diagnosis.

Objective To conduct a pilot study on genome-wide in vivo protein-RNA interactions in E.coli.Methods Bacterial lysate was treated with RNase before the RNA fragments protected by proteins were extracted from treated lysate and used to construct cDNA library that was applied to high-throughput sequencing .Finally, the transcripts bound by proteins were obtained by bioinformatics analysis .Results A total of 3193 transcripts were obtained , including 2234 mRNAs, 47 sRNAs, 39 tRNAs, 11 rRNAs, and 862 intergenic regions .Conclusion Some information of transcripts interacting with proteins in E.coli is acquired , which will facilitate further studies of protein-RNA interactions .

Objective To study the local irritation of repeated intrathecal injection of Ziconotide Acetate,and to provide reference for irritancy evaluation ofintrathecal injection.Methods Sixteen New Zealand rabbits were assigned into two groupsat random:Control group and Ziconotide Acetate group,eight animals each group.Totally 50 μL saline or Ziconotide Acetate (100 μg/mL) were administrated by repeated lumbar intrathecal injection once daily for 7 d.Animal behavior was observed every day,and four animals in each group were sacrificed 2 d later after the last injection,the lumbar spinal cord was removed for histopathological examination and irritancy evaluation.The remaining animals were sacrificed for initancy evaluation 14 d later after the last injection.Results Only one animal died after anesthesia on day three in saline group,while no obvious adverse reactions were observed in other rabbits during the entire study,and no intrathecal irritant reactions of histopathological examination were found in both groups.The reversible minor mechanical damage was observed at the injection point,2 d after the last administration.Conclusion For 7 d repeated lumbar intrathecal injection in rabbits,no intrathecal irritant reactions observed in Ziconotide group,and the New Zealand rabbit could be used as a local irritation evaluation model.

Objective To provide the toxicity basis for safety evaluation of Polyunsaturated Fatty Acids.Methods Acute toxicity test,micronucleus test of born marrow in mice,Ames test,sperm shape abnormality and thirty-day feeding test in rats were conducted in this study.Results The oral LD50 of Polyunsaturated Fatty Acids in both mice and rats were more than 20.0g/kg.BW,so the Polyunsaturated Fatty Acids was classed as actual non-toxicity.The results of micronucleus test of born marrow in mice,Ames test,sperm shape abnormality and thirty-day feeding test in rats were negative.The thirty-day feeding test in rats demonstrated that it had no obvious toxic effects on routine blood,body weight,and biochemical index.No obvious adverse effects dose of this sample was 6.66g/kg.BW.Conclusion Polyunsaturated Fatty Acids toxicity test results showed no obvious toxicity and was safe for edibility.

Objective To study tea polysaccharides(TP) on glucose metabolism,histopathology,and pancreatic islet ?-cell ultrastructure in diabetic mice.Method The alloxan diabetic mice were randomly divided into 4 groups,and orally given distilled water,TP 0.25,0.50,1.00 g/(kg bw?d).for 5 w,and weighed once every week.In experiment 2 and 4 w,the fasting blood glucose was tested once.The glucose tolerance test was conducted at the end of experiments.Blood serum insulin and liver glycogen were measured.The protein content,hexokinase(HK) and pyruvate kinase(PK) activity of 10% liver homogenate were measured.The histopathology of liver,pancreas,kidney and spleen tissue and the ultra structure of pancreas were observed.Results TP could significantly alleviate the symptoms of diabetic mice.The fasting blood glucose values in three TP groups were significantly decreased(P