1.Evaluation of left ventricular function in children with dilated cardiomyopathy using quantitative tissue velocity imaging(QTVI)
Journal of Chongqing Medical University 2003;0(05):-
Objective:To evaluate the application of Quantitative tissue velocity imaging(QTVI) in assessing the left ventricular (LV) systolic function in Children with dilated cardiomyopathy(DCM).Methods:Fifteen consecutive children with DCM were included in the present study,who were divided into group A(with light heart failure) and group B(with moderate and severe heart failure).Twelve children without any cardiac diseases were as controls.The dynamic tri-plane QTVI images of apical four-chamber,LV two-chamber and LV long-axis views were acquired simultaneously and the myocardial velocity curves in basal and middle segments were analyzed.Results:(1)The peak systolic velocity (Vs)redueed generally in group A and B compared with that in controls,especially in group B(P0.05).(2)There were forty-two segments with the velocity of postsystolie shortening(PSS) more than that of the systole in DCM group,among which thirty segments(71.43%)were from group B,and twelve segrnents(28.57%)from group A. Analyzed with strain,twenty-one segments (50.00%)were actively shortened and eleven segments (26.19%)were paradoxical motion.Conclusion:QTVI can assess LV systolic function accurately in Children with DCM.
2.Analysis of bacteriology of drug sensitivity in the inactive stage of chronic suppurative otitis media.
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(13):932-934
OBJECTIVE:
Detecting the sensitivities of bacterium in the inactive stage of chronic suppurative otitis media, to guide a clinical rational drug use after the type I tympanoplasty.
METHOD:
Collecting 148 hospitalized patients from Guangdong General Hospital diagnosed as inactive stage of chronic suppurative otitis media, who had been dry ear for more than 1 months. Analyse the results of drug sensitivity on the mucosa surfaces of the middle ear.
RESULT:
There are 16 species of bacterium, including Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter cloacae etc. Total positive rate of bacteria was 33.1%, the detectable rate of gram-positive bacteria was 69.4%, gram-negative bacteria was 30.6%. Drug sensitive test demonstrated that vancomycin, linezolid, teicoplanin, rifampicin etc were effective in gram positive bacteria, piperacillin/tazobactam, cefoperazone/sulbactam etc were effective in gram-negative bacteria.
CONCLUSION
Patients with dry ears,who were going to take type I tympanoplasty, still had potential pathogens. Bacterial cultivation and drug sensitive test before operation can guide the clinical medication and prevent infection.
Adolescent
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Adult
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Aged
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Anti-Bacterial Agents
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pharmacology
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Chronic Disease
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Drug Resistance, Bacterial
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Female
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Gram-Negative Bacteria
;
drug effects
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Gram-Positive Bacteria
;
drug effects
;
Humans
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Male
;
Microbial Sensitivity Tests
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Middle Aged
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Otitis Media, Suppurative
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microbiology
;
Young Adult
3.Effect of Osthole on Mast Cells and Expression of STAT5 Gene and Protein in Mice with Eczema
Jian XIONG ; Zhendong ZHONG ; Rong FU ; Wei XIONG
Herald of Medicine 2015;34(12):1584-1587
Objective To explore the effect of osthole on mast cells and expression of STAT5 gene and protein in them. Methods Passive cutaneous anaphylaxis was made in mice to copy eczema model, then the allergic mast cells were separated, and the ovalbumin was used to induce allergy of mast cells. Different concentrations of osthole were used to intervene the sensitized mast cells.Then the sensitized mast cells were divided into blank control group, osthole high-dose group and low-dose group.At the end of the experiment, morphology of the mast cells was detected by immunofluorescence technology.MTT assay was used to detect the influence of drugs on mast cells proliferation. RT-PCR and Western blotting were used to detect the expression of STAT5 gene and protein. Results As compared with blank control group, the number of mast cells in the osthole groups was significantly reduced, cells and nuclei obviously shrank, even apoptosis of some cells could be observed; the inhibition rate of mast cells in osthole groups was significantly increased in concentration-dependent manner ( P<0. 01 ) . As compared with blank control group (2.16±0.57), gene expression of STAT5 was significantly decreased in osthole high-dose group (0.59±0.12) and low-dose group (0.82±0.13) (P<0.01).The protein expression of STAT5 was also significantly decreased in osthole high-dose group and low-dose group as compared with blank control group (P<0.01). Conclusion Osthole can inhibit the proliferation of sensitized mast cells, and reduce the expression of STAT5 gene and protein.
4.Expression of KAI1 gene in cervical cancer and its clinical significance
Xia LIN ; Xiaoying XIONG ; Lihua FU
Chinese Journal of Primary Medicine and Pharmacy 2006;0(06):-
Objective To study the expression of KAI1mRNA in cervical carcinoma and its clinical significance.Methods The transcription-polymerase chain reaction(RT-PCR) method was applied to detect the expression of KAI1mRNA in 53 fresh freezing cervical tissues.The findings were compared with the clinicopathological data.Results The expression of KAI1mRNA was (0.309?0.093) in normal cervix,(1.608?0.289) in cervical intraepithelial neoplasia(CIN),(0.948?0.910) in cervical carcinoma respectively.The relative densitometric value of KAI1mRNA decreased in late stage of cervical cancer in local cervical cancer with lymphatic metastasis(P
5.Application of protein transduction in gene therapy
Fu XIONG ; Cheng ZHANG ; Meijuan YU
International Journal of Biomedical Engineering 2006;0(04):-
Protein transduction is an emerging technique in biological fields in recent years. Through the medium of protein transduction domain(PTD) composed by a small number of alkaline peptides, the functional proteins linked covalently with DNA, peptides, proteins or other macromolecules can across the membranes of mammalian cells, even the blood-brain barrier and transfer unconventionally that isn't like classical pathway. The distinct property of PTD that can take along fused protein entering into cells unconventionally offers a new vehicle to gene therapy for some diseases and has dulcet applied perspective.
6.Effects of Wulong Granules on the Interlerkin mRNA Expression of Nasal Mucosa in Alergic Rhinitis Rats
Wenyang FU ; Hiu XIE ; Dajing XIONG
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(03):-
Objective To study the effect of Wulong granules on the expression of IL-2 mRNA,IL-4 mRNA and IL-6 mRNA in allergic rhinitis(AR) rats in Spleen Qi deficiency type. To establish the clinic treatment principle-tonify Spleen and reinforce Qi of AR through the lab study. Methods Established AR model with combination of disease and Zheng,checked the expression of IL-2 mRNA,IL-4 mRNA and IL-6 mRNA of nasal mucosa were detected with RT-PCR. Results Wulong granules can adjust the expression of mRNA of AR rats' nasal mucosa. It can descend the ascensus of IL-4 mRNA,IL-6 mRNA and the drop of IL-2 mRNA. Conclusion Wulonggranules can show treatment effect to AR rats through adjusting the expression of cell factors such as IL-2 mRNA,IL-4 mRNA and IL-6 mRNA.
7.Cloning and sequencing of the CTX-M-12 encoding gene of ?-lactamase
Zizhong XIONG ; Demei ZHU ; Fu WANG
Chinese Journal of Infectious Diseases 2000;0(02):-
Objective To investigate the ESBL gene in a Klebsiella pneumoniae strain isolated from a patient in Huashan Hospital, Shanghai. Methods ESBL gene in the transconjugant from Klebsiella pneumoniae isolate was amplified by PCR. The PCR products were cloned into pHSG398 vector. The antibiotic susceptiblity and ?-lactamases activity of the clone strain were tested; The PCR product was sequenced and analyzed. Results The ESBL gene was identified as CTX-M-12 gene and CTX-M-12 is much more active against cefotaxime than against ceftazidine.Conclusions A clinical isolate of Klebsiella pneumoniae k99-442 isolated from a patient in Huashan Hospital, Shanghai produced ESBL type CTX-M-12 which caused this isolate resistant to most ?-lactams.
8.Application of temporary cardiac pacemaker in perioperative period of laparoscopic cholecystectomy
Jianping FU ; Leping XIONG ; Xiaohua WANG
Chinese Journal of Minimally Invasive Surgery 2005;0(07):-
Objective To evaluate the safety of temporary cardiac pacemaker during perioperative period of laparoscopic cholecystectomy (LC) in patients with bradyarrhythmia. Methods Temporary cardiac pacemakers were installed preoperatively in 34 elderly patients with bradyarrhythmia for LC. On operation the ultrasonic harmonic scalpel, instead of electrotome, was used to perform dissection and coagulation. Results LC was completed successfully in all the 34 patients without surgery-related complications or perioperative serious cardiovascular diseases. Conclusions Utilization of temporary cardiac pacemaker and ultrasonic harmonic scalpel may considerably enhance the perioperative safety and reduce the incidence of cardiovascular complications in elderly patients with bradyarrhythmia.
9.Deep Fungal Isolates from Hospital-acquired Infection in Tumor Patients after Chemotherapy
Jianqiu XIONG ; Hong HE ; Ronghua FU
Chinese Journal of Nosocomiology 2006;0(02):-
OBJECTIVE To investigate the pattern of fungal isolates from hospital-acquired infection in tumor patients after chemotherapy. METHODS The clinical information of fungus isolates from 102 tumor patients after chemotherapy was investigated.We analyzed the risk factors,species and drug-resistance of isolates. RESULTS The lower respiratory tract and mouth infection accounted for 61.8% and 16.7% in 102 cases with fungal infection and 102 isolates were isolated from various clinical specimens.Among above 102 isolates,Candida albicans and C.tropicalis accounted for 71.6% and 14.7%.The resistant rate of C.albicans to fluconazole and miconazole were 96.2% and 61.4%,respectively. CONCLUSIONS Hospital-acquired infection resulted from fungi in tumor patients after chemotherapy is related to primary tumor,age,the amount of white cell and use of antibiotics.
10.Laparoscopic and open treatment for choledocholithiasis:a prospective clinical comparative study
Xiaochu ZHOU ; Pei XIONG ; Jianghua FU
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To compare the therapeutic results of laparoscopic and open treatment for choledocholithiasis. Methods A prospective comparison was conducted between 29 cases of laparoscopic choledocholithotomy and 35 cases of open choledocholithotomy in terms of stone removal,operative time,hospitalization day,hospitalization cost and postoperative complications. Results Stone removal was achieved in 28/29 patients by a laparoscopic approach and in 32/35 patients by a open approach( ? 2=0 71, P =0 399);average operative time was (178 3?32 4) min in laparoscopic approach and (145 8?27 1)min in open approach( t =4 27, P =0 01);the patients in laparoscopic procedures had flatus in (20 8?2 9) hour and those in open procedures did in (58 2?4 9) hours( t =37 8, P 0 05);no major complication was found in laparoscopic approach. Conclusions Laparoscopic choledocholithotomy is safe and effective and it is superier to open treatment.