This article was aimed to interpret the composition of Mahuang Decoction. The analysis was made on key points of medication, herbal combination, weigh ratio, the combined prescription treatment of Taiyang cold damage syndrome (wind-cold exterior-excess pattern) and heart-heat syndrome, heart-yang deficiency, heart-blood defi-ciency, heart-yin deficiency, liver-heat syndrome, liver-cold syndrome, liver-blood deficiency, liver-yin deficiency, spleen-stomach stagnated heat syndrome, spleen-stomach cold deficiency, spleen-stomach qi and yin deficiency, lung-heat syndrome, lung-cold syndrome, lung-yin deficiency, lung qi and yin deficiency, kidney qi insecurity, kid-ney-yang deficiency, kidney-yin deficiency, kidney yin-yang deficiency syndrome. Attention should be paid to use Mahuang Decoction as the combined prescription to receive the best treatment effect in the clinical practice.

Objective To investigate the effect of RNAi-mediated survivin gene with conditionally replicating adenovirus Ad-de-lE1b55KD-shRNA/survivin-EGFP silencing on propagation and apoptosis of colon carcinoma cell lines HT-29 .Methods Ad-de-lE1b55KD-shRNA/survivin-EGFP was transfected to transplantation tumor on athymic mouse ,replication defective adenovirus and liposome vector which was contained the same shRNA as Ad-delE1b55KD-shRNA/survivin-EGFP were as control one .Then the change of transplantation tumor on athymic mouse was tested ,and TUNEL was used to assay the apoptosis rate of transplantation tumor cells .Results After transfection of Ad-delE1b55KD-shRNA/survivin-EGFP ,the quality of transplantation tumor on athymic mouse were reduced and the apoptosis rate of transplantation tumor cells were higher than replication defective adenovirus and lipo -some vector which was contained the same shRNA (P<0 .05) .Conclusion The effect of Ad-delE1b55KD-shRNA/survivin-EGFP to colon carcinoma cell on propagation and apoptosis were higher than replication defective adenovirus and liposome vector .

Marine medicine is an important part of Chinese medicine and a growth point of the development of marine economy. Current researches of marine medicine had ignored accumulated experience in clinical medicine for thousands of years of development of marine medicine and the guidance of traditional Chinese medicine (TCM) theory. In this article, we proposed a research mode of Mining experience-Clinical validation-Targets screening-Component screening-Component compatibility-Mechanism study under the guidance of the hypothesis of Nature-Effect-Material Trialism to study marine medicine. Kun Hai Jiang Xin (KHJX) Decoction is a marine formula drawn from the experience of ancient physicians by data mining techniques. Clinical study found that KHJX Decoction can significantly improve the overall clinical efficacy of chronic bronchitis with good clinical safety. Under the guidance of TCM herbal theory, object presented here is based on the work of KHJX Decoctionmining experience - clinical validation. The molecular docking method and receptor-ligand binding assay were applied to screen effective components from herbs of KHJX Decoction and the compatibility plan of effective components under the guidance of the principle of cold and heat effects compatibility. This study is of great significance to clarify the material basis and molecular mechanism of KHJX Decoction in the treatment of chronic bronchitis. It provides a new research mode for marine drug research.

Hepatic hemangioma is one of the most common benign tumor,It mainly occour in middle-aged women. With the development of medical technology and the improvement of standard living, the discovery rate haihger than before. There are different opinion in different scholar about the choice of surgical treatment. This paper summarizes the surgical treatment and operative indication of hepatic hemangioma.

Case based teaching or case discussion teaching of basic medical courses can deepen students' understanding of basic medical subjects and allow them to acquire practical and real problem solving skills while leaning the fundamental knowledge of their subjects.This paper discusses the features and advantages of case based teaching and gives a detailed discussion of an effective way to implement case based teaching by taking case based teaching of medical immunology as an example.

BACKGROUND: Some studies have shown that bone marrow mesenchymal stem cells and al ograft bone have a certain role for repairing bone defects, but the effectiveness on cancel ous bone defects is seldom reported so far. OBJECTIVE: To observe the effectiveness of bone marrow mesenchymal stem cells combined with al ogeneic bone on cancel ous bone defects. METHODS: The models of cancel ous bone defects (0.6 cm×1.2 cm) were made artificial y in both condylus lateralis femoris of New Zealand white rabbits: one side served as model group implanted with combination of bone marrow mesenchymal stem cells and al ogeneic bone, and the other side was considered as control group implanted with al ogeneic bone. RESULTS AND CONCLUSION: The model group was better than the control group in new bone growth and defect repair at 4, 8, 12 weeks after implantation, which was confirmed by general observation, X-ray examination and hematoxylin-eosin staining. There was a large amount of trabecular bone formation and mature lamel ar bone tissue in bone defects of model group by histological observation at 12 weeks after implantation, and bone defects of the model group were repaired basical y; while there were only abundant woven bones in the control group, and bone defects in the control group were not repaired effectively. Scores on Lane-Sandhu’s X-ray combined with histological observation were higher in the model group than the control group (P < 0.05). Biomechanical test showed that the maximum pressure load of the femoral condyle and load/strain ratio in the model group were significantly higher than those in the control group at 12 weeks after implantation (P < 0.05),while the maximum strain and displacement of the model group was lower than that of the control group (P < 0.05). These findings suggest that the combination of bone marrow mesenchymal stem cells and al ogeneic bone is superior to simple al ogeneic bone implantation in the repair of cancel ous bone defects of the femoral condyle.

Rectal carcinoid tumors are potential malignant and have been considered to be rare for a long time.But in recent years,the incidence has been increasing gradually,maybe due to the development of endoscopic technology,the widespread use of endoscopy for screening and the raising level of awareness.Because of the malignant tendency,early diagnosis and treatment are the most important things for patients.Nowadays,the misdiagnosis rate is still high and it is difficult to make differential diagnosis between benign and malignant tumors.The efficiency of chemotherapy and radiotherapy is not clear.Surgery is the only effective method,but there is no uniform standard for the choice of operation.At present,it is appropriate to consider tumor size,depth of invasion,lymph node,pathological type and other risk factors to determine a surgical modality.Colonoscopy is recognized to be the best means by follow-up and should be carried out regulady in a longer period of time.Therefore,it is important to summarize the diagnosis and treatment of rectal carcinoid.

OBJECTIVE:To investigate the use of antibiotics in our hospital.METHODS:The data regarding the use of antibiotics retrieved in July 2006 from 184 discharged histories were analyzed retrospectively.RESULTS:The application rate of antibiotics in our hospital reached 77.3%,of which,34.6% were used in combination of two kinds,and 32.6% were used in combination of three kinds.Only 5.9% of the specimens had been sent for pathologic examination.CONCLUSION:The application rate of antibiotics in the inpatients of our hospital as well as the postoperative use of antibiotics were all on the high side,while the rate of specimens sent for pathologic examination was on the low side,therefore,it is urgent to strengthen control on the rational use and standardized management of antibiotics.

OBJECTIVE: To establish an HPLC method for the determination of Matrine in Baishan tablets. METHODS: The analytical column was Aminopropyl (200 mm?4.6 mm, 10 ?m) with mobile phase consisted of acetonitrile-0.5% phosphate solution - ethanol(100∶8∶10) at a flow rate of 1.0 mL?min-1. The column temperature was set at 30 ℃ and the detection wavelength was set at 220 nm. RESULTS: The linear range of Matrine was 0.27～ 8.66 ?g(r=0.999 8) and the average recovery of Matrine was 99.52 %(RSD=0.91%,n=6). CONCLUSION: The method is simple, accurate and reproducible, and it is applicable for the quality control of Baishan tablets.

Objective: To study the mental health status and influential factors of elderly people in the rural areas. Methods: 714 elderly people were tested by the SCL-90. Results: All the factor scores of SCL-90 of the elderly people in the rural areas were higher than those of the retired people. Some factor scores of SCL-90 of elderly people showed significant differences because of existence of spouse, physical health, filial piety of sons and daughters, and economic independence. Conclusion: The mental health status of elderly people in the rural areas is poorer than that of retired people. Existence of spouse, physical health, filial piety of sons and daughters and economic independence all influence the mental health of elderly people in the rural areas.