This article was aimed to interpret the composition of Mahuang Decoction. The analysis was made on key points of medication, herbal combination, weigh ratio, the combined prescription treatment of Taiyang cold damage syndrome (wind-cold exterior-excess pattern) and heart-heat syndrome, heart-yang deficiency, heart-blood defi-ciency, heart-yin deficiency, liver-heat syndrome, liver-cold syndrome, liver-blood deficiency, liver-yin deficiency, spleen-stomach stagnated heat syndrome, spleen-stomach cold deficiency, spleen-stomach qi and yin deficiency, lung-heat syndrome, lung-cold syndrome, lung-yin deficiency, lung qi and yin deficiency, kidney qi insecurity, kid-ney-yang deficiency, kidney-yin deficiency, kidney yin-yang deficiency syndrome. Attention should be paid to use Mahuang Decoction as the combined prescription to receive the best treatment effect in the clinical practice.

Marine medicine is an important part of Chinese medicine and a growth point of the development of marine economy. Current researches of marine medicine had ignored accumulated experience in clinical medicine for thousands of years of development of marine medicine and the guidance of traditional Chinese medicine (TCM) theory. In this article, we proposed a research mode of Mining experience-Clinical validation-Targets screening-Component screening-Component compatibility-Mechanism study under the guidance of the hypothesis of Nature-Effect-Material Trialism to study marine medicine. Kun Hai Jiang Xin (KHJX) Decoction is a marine formula drawn from the experience of ancient physicians by data mining techniques. Clinical study found that KHJX Decoction can significantly improve the overall clinical efficacy of chronic bronchitis with good clinical safety. Under the guidance of TCM herbal theory, object presented here is based on the work of KHJX Decoctionmining experience - clinical validation. The molecular docking method and receptor-ligand binding assay were applied to screen effective components from herbs of KHJX Decoction and the compatibility plan of effective components under the guidance of the principle of cold and heat effects compatibility. This study is of great significance to clarify the material basis and molecular mechanism of KHJX Decoction in the treatment of chronic bronchitis. It provides a new research mode for marine drug research.

Aged ; Apraxias ; physiopathology ; Gait Apraxia ; physiopathology ; Humans ; Parkinson Disease ; diagnosis ; physiopathology

Objective To construct the conditionally replicating adenovirus vector Ad-delE1b55kD-shRNA/Survivin-EGFP that can transfects into HT-29 effectually and selectively and contains shRNA targeting to human Survivin gene.Methods EGFP geoe and shRNA gene were inserted into pAd-delE1b55kD,then pAd-delE1b55kD-shRNA/Survivin-EGFP was obtained.The plasmid and pBHGE3 were cotransfected into HEK-293 cell to obtain the conditionally replicating adenovirus vector Ad-delE1b55kD-shRNA/Survivin-EGFP.Then DNA of the adenovirus vector was extracted and identified.The transfection efficiency of the vector to HT-29 and LO2 was detected.The expression of Survivin mRNA and protein was detected by RT-PCR and Western blot.Results EGFP gene and shRNA gene targeting to human Survivin mRNA were inserted into conditionally replicating adenovirus vector successfully,proven by sequencing.The transfection efficiency to HT-29 of Ad-delE1b55kD-shRNA/Survivin-EGFP was significantly higher than replication defective adenovirus and liposome vector(P

Sorafenib,a novel oral multikinase inhibitor,targts on serine/threonine kinase and tyrosine kinases receptor of the tumor cells and vasculature.So Sorafenib can inhibit the tumor cell proliferation and revascularization.400 mg sorafenib administered twice a day is chosen as the recommended dosage for several phase Ⅰ studies,and it shows broad-spectrum antitumor activity in renal cancer,hepatocellular carcinoma,melanoma and non-small-cell lung cancer(NSCLC)in phase Ⅱ and Ⅲ clinical trials.Now FDA has approved the usage of sorafenib in renal cancer therapy.

[Objective]To investigate the effects of bone marrow stromal stem cells(MSCs) on the bone defect around the implant and the osteointegration of implant-bone interface.[Method]Fifteen healthy clean New Zealand rabbits were studied.MSCs were separated,cultured and induced to osteoblasts.Cancellous bone defects(0.6?1.2 cm)were created in bilateral femur condyle in rabbits.Titanium alloy columns(0.3?1.0 cm)were implanted into both defects.Tissue engineering bone was implanted around the left titanium alloy implant,but only hydroxyapatite ceramic in the right.The biological characteristics were evaluated by X-ray examination,SEM,energy dispersive X-ray analysis and histologic studies at 4,8,and 12 weeks postoperatively.[Result] X-ray examination showed the density of bone tissue around the implant was aequalis.The interspace between implant and bone was filled with lots of bone trabeculae in the experimental group at 12 weeks postoperatively.The density of bone tissue was inhomogeneous,and low-density umbra existed in the interspace in the control group.Energy dispersive X-ray analysis showed the content of the Ca and P in the experimental group was statistically higher than that in the control group at different time points(P0.05.The ratio of Ca to P became higher and reaching the peak level at 8 week.Histological study showed the bone defects around the implant were repaired by new mature bone in the left at 12 weeks postoperatively,while in the right no new bone was found at any time point.[Conclusion]The compounds of hydroxyapatite ceramic and osteoblasts induced by MSCs can repair the bone defect around the implant and improve the osteointegration of implant-bone interface.

[Objective]To dynamically monitor revascularization and metabolism of tissue-engineered bone graft in repairing bone defects by radionuclide bone imaging.[Method]Twenty-four healthy New Zealand rabbits were studied.Mesenchymal stem cells(MSCs) were separated and cultured from bone marrow.Cancellous bone defects(0.6 cm?1.2 cm) were created on bilateral femoral condyles in rabbits.The compound of coral hydroxyapatite ceramic(CHA) and defects were treated with the induced osteoblasts inplanted into the left defects(experimental group).While CHA alone was used for the right(control group).The counts of regions of interest(ROI) was evaluated by radionuclide bone imaging at 4,8 and 12 weeks postoperatively.[Result]ROI in the experimental group was significantly higher than that of control group at 4,8 and 12 weeks postoperatively.ROI in either group had a tendency to increase with time.Eight weeks postoperatively,ROI in experimental group tended to increase at a lower speed,while ROI in control group had a tendency to increase at a higher speed.ROI in either group reached a peak at 12 weeks postoperatively.[Conclusion]The tissue-engineered bone graft is an effective method for repairing cancellous bone defects.Radionuclide bone imaging can dynamically monitor the changes of revascularization and metabolism of tissue-engineered bone graft.

Objective To observe the impact of pulse pressure (PP) and blood pressure (BP) circadian rhythm abnormality of aged hypertension patients on carotid atherosclerosis. Methods One hundred and fifty cases of elderly patients with essential hypertension based on 24 h ambulatory blood test results, according to the circadian blood pressure rates were divided into two groups,<10% in the non-dipper type hypertension group (72 cases),≥10%in the dipper type hypertension group (78 cases). Carotid ultrasound was performed to detect the extent, nature and classification of atherosclerotic plaques. The detection rate of carotid artery plaque in patients with hypertension was calculated based on their pulse pressure levels:≤60 mmHg (1 mmHg=0.133 kPa), 61~89 mmHg, ≥90 mmHg. Results Ultrasonography showed 61 cases of carotid artery plaques in the dipper type hypertension group, 20 cases were located bilaterally, 15 cases were in the right, 26 cases were in the left, the detection rate of plaque was 78%(61/78). Ultrasonography showed 68 cases of carotid artery plaques in the non-dipper type hypertension group, 38 cases were located bilaterally, 14 cases were in the right, 16 cases were in the left, the detection rate of plaque was 94%(68/72). The detection rate of plaque in the dipper type hypertension group was less than that in the non-dipper type hypertension group, the difference was statistically significant (χ2=8.07, P<0.01). There were 30 cases of hard plaque,13 cases of soft plaque, 8 cases of mixed plaque, and the plaques graded as 0, 1, 2, 3 were 17, 33, 13, 15 cases in dipper type hypertension group. There were 12 cases of hard plaque, 24 cases of soft plaque, 32 cases of mixed plaque, and the plques graded as 0, 1, 2, 3 were 6, 8, 25, 33 cases in non-dipper type hypertension group. The difference was statistically signiifcant (χ2=5.29, 8.75, both P < 0.01). Forty three patients with pulse pressure≤60 mmHg, the plaque detection rate was 72%(30/43);the pulse pressure of 65 cases was 61~89 mmHg, the plaque detection rate was 88%(57/65);42 cases with vein pressure ≥ 90 mmHg, the plaque detection rate was 98%(41/42). The difference was statistically signiifcant (χ2=11.65, P=0.00). Conclusion Pulse pressure increased and BP circadian rhythm abnormality are risk factors for the formation of carotid atherosclerosis.

AIM: To investigate autofluorescence (AF) images of classical retinitis pigmentosa (RP) at advanced stage correlated with central vision. METHODS: Thirty-seven patients (70 eyes) with classical RP at advanced stage were observed. AF imaging was performed using a Heidelberg Retina Angiograph 2 ( HRA-2) scanning laser system with the field 30°, the pixels 1 536×1 536 and pupil diameters >6mm. Some patients underwent automated static peri-metry and the flash electroretinogram(FERG) were performed according to International Society for Clinical Electrophysiology of Vision( ISCEV) standard. RESULTS: All patients were classified as four patterns according to their manifestation of AF they were intact, patchy, solitary island and central hypofluorescence patterns. Different pattern showed different central vision. The data demonstrate high correlation between the central cone function and AF changes of the fundus. There is significant correlation (n= 57, r= 0.385,P＜0.01) between central vision and the AF area and between age and AF area (n= 57, r= -2.67,P＜0.05).CONCLUSION: AF images is valuable to the monitoring of central cone function and in predicting retention of central vision. The changes of AF images correlated with central vision and different pattern showed different vision.

OBJECTIVE:To investigate the use of antibiotics in our hospital.METHODS:The data regarding the use of antibiotics retrieved in July 2006 from 184 discharged histories were analyzed retrospectively.RESULTS:The application rate of antibiotics in our hospital reached 77.3%,of which,34.6% were used in combination of two kinds,and 32.6% were used in combination of three kinds.Only 5.9% of the specimens had been sent for pathologic examination.CONCLUSION:The application rate of antibiotics in the inpatients of our hospital as well as the postoperative use of antibiotics were all on the high side,while the rate of specimens sent for pathologic examination was on the low side,therefore,it is urgent to strengthen control on the rational use and standardized management of antibiotics.