OBJECTIVETo observe differences of clinical therapeutic effects between deep-oblique acupuncture with long needle and regular perpendicular acupuncture at Guanyuan (CV 4) for urinary retention to explore standard acupuncture method in clinic.

METHODSOne hundred and thirty-two cases of urinary retention were randomly divided into an observation group (78 cases) and a control group (54 cases). Needles with 75 mm in length were deeply and obliquely inserted into Guanyuan (CV 4) in the observation group while needles with 40 mm in length were perpendicularly inserted. Clinical therapeutic effects in two groups were observed.

RESULTSAfter treatment the total effective rate was 80.8% (63/78) in the observation group and 57.4% (31/54) in the control group, which had significant difference between two groups (P<0.05). The curative effect of postpartum retention of urine was obviously superior to that of postoperative and prostate-induced urinary retention in the ebservation group, and the effect of urinary retention due to different etiology in the observation was also superior to that in the control group in the ebservation group [in the observation group total effective rate was 88.6% (47/53) in postpartum retention of urine, 50.0% (3/6) in prostate-induced urinary retention and 68.4% (13/19) in orthopedic postoperative retention of urine while in the control group total effective rate was 69.4% (25/36) in postpartum retention of urine, 20.0% (1/5) in prostate-induced urinary retention and 38.5% (5/13) in orthopedic postoperative retention of urine)].

CONCLUSIONThe deep-oblique acupuncture with long needle at Guanyuan (CV 4) has significant curative effect on urinary retention in clinic, especially for patients with postpartum urinary retention, which could be taken as standard acupuncture method.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Urinary Retention ; therapy

Antirheumatic Agents ; therapeutic use ; Arthritis, Rheumatoid ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Etanercept ; Female ; Humans ; Immunoglobulin G ; therapeutic use ; Male ; Methotrexate ; therapeutic use ; Phytotherapy ; Prednisone ; therapeutic use ; Receptors, Tumor Necrosis Factor ; therapeutic use

Objective To observe the therapeutic efficacy of centro-square needling plus stuck needling with lifting-thrusting manipulations in treating the injury of medial collateral ligament (IMCL) of knee joint. Methods Seventy-eight patients with IMCL of knee joint were randomized into a treatment group of 40 cases and a control group of 38 cases. The treatment group was intervened by centro-square needling plus stuck needling with lifting-thrusting manipulations, while the control group was by local anesthesia treatment. The therapeutic efficacies were compared between the two groups. Results The total effective rate was 97.5% in the treatment group versus 81.6% in the control group, and the difference was statistically significant (P＜0.05). Conclusion Centro-square needling plus stuck needling with lifting-thrusting manipulations is an effective way in treating IMCL of knee joint.

Objective:Explore decoy oligonucleotide (ODN) technology targeted blocking STAT3 activation way effects on colorectal cancer HT29 cell growth,to provide experimemt basis for gene therapy of colorectal cancer.Methods:Using decoy ODN in vitro transfect colorectal cancer HT29 cell line,to targeted blocking STAT3 activation way.Fluorescence microscope and flow cytometry detect transfection conditions and efficiency,and MTT method detect the cell growth inhibition,Realtime RT-PCR and Western blotting test STAT3 in cells,the Bcl-xl and Caspase3 mRNA and protein expression.Results:STAT3 decoy ODN can efficient transfection into colorectal cancer cells,and is due to the nucleus.HT29 cell in the STAT3 decoy ODN,growth inhibition rate increased significantly,and mRNA and quantity of protein expression of STAT3 significantly reduce (P＜0.05),while caspase3 increase significantly (P＜0.05).Conclusion:Named Decoy ODN targeted after blocking STAT3 activation way,which can effectively cut colorectal cancer cells HT29 STAT3 gene expression,and inhibit the growth of the cells.The mechanism may be related to lower the expression of STAT3 and the Bcl-xl and Caspase3.

Corneal collagen cross-linking (CXL) is a photochemotherapy for keratoconus, keratectasias and infectious keratitis.Corneal collagen fiber infiltrated with riboflavin, a photosensitizer which generates reactive oxygen species when activated by UVA at 370nm, was induced to form the crosslinks in corneal stroma.Corneal collagen cross-linking alone or combined with medicine therapy could be used to treat fungal keratitis, improve the cure rates, and reduce the complications and the demands of corneal transplantation surgery.The purpose of this paper is to review the basic principle, procedure, laboratory researches, clinical applications and the safety about this treatment.

Objective To evaluate the investigation outcome of eliminating iodine deficiency disorders(IDD)in Ya'an city of Sichuan province,and to master the current situation of IDD in the whole city.Methods In 2009,according toCounties'Assessment Guideline in Sichuan for Implementing the Measure to Eliminate Iodine Deficiency Disorders,assessments were conducted.At county level,composite score was given to collected data of the eight counties(districts)and monitoring data of household iodized salt were statistically analyzed.A township (town)was randomly selected by east,west,south,north and center locations,respectively.One primary school was randomly selected in each selected township(town),eighty children aged 8 to 10 in each selected school were checked of their thyroid by palpation(WS 276-2007)and urinary iodine level of20 children among them was measured by As3+_Ce4+ catalytic spectrophotometry(WS/T 107-2006).Thirty fifth-grade students and 10 housewives lived around school were asked to take a questionnaire survey.Results Average score of the 8 counties(districts)was 89.96,ranged from 85.42 to 92.90.The coverage rate of iodized salt of household was 100.00%(2328/2328),rate of qualified iodized salt was 97.98%(2281/2328).and consumption rate of qualified iodized salt reached 97.98%(2281/2328).The goiter rate of children aged 8-10 was 0.91%(33/3620),median urinary iodine was 235.85μg/L,and 1.50%(12/800)of child's urinary iodine was less than 50μg/L.The rate of passing a knowledge questionnaire survey of the fifth-grade students and women was 92.33%(1 131/1225)and 9413%(397/421),respectively.Conclusions Ya'an city in Sichuan province has reached the goal of eliminating iodine deficiency.disorders,but there is a coexist trend of IDD and iodine excess.They should pay attention to the daily consumption of iodized salt in the future;sustained elimination of IDD can be reached only by iodine supplementation scientifically.

Objective To evaluate the left ventricular function in patients with different extents of coronary stenosis by the left ventricular pressure volume loop Methods In 65 patients who were undergoing coronary angiography and left ventriculography examinations, materials of clinical and cardiac catheter examinations were collected In right anterior oblique 30 degrees the left ventriculography was progressed The pressure curves of the left ventricle and the aorta were recorded continuously and volumes of the left ventricles were measured with the dot tracing method Pressure volume loop was set up and ventricular energy indexes embodied by the pressure volume loop were measured and calculated Results In normal group, pressure volume loop was located in the left and lower portion of the coordinate system Along with the levels of the coronary stenosis becoming more severe, pressure volume loops moved to the right and upper portion of the coordinate system Little changes occurred in ejection fraction except that there was a decreasing in patients with lesions of three branches; Stroke work showed no obvious changes; Filling energy became larger; Total energy increased obviously in both groups with lesions of double branches and three branches; End systolic energy increased gradually while energy efficiency decreased gradually Conclusion The ventricular pressure volume loop can be obtained in routine ventriculography which can reflect many indexes of ventricular function quantitatively The ventricular energy indexes change correspondingly with coronary artery lesions and may be useful to assess ventricular function in patients with different levels of coronary stenosis

OBJECTIVE To investigate the distribution and resistance of non-fermenting bacteria isolated from(patients) in 2005 and offer a basis for the treatment of bacterial infection.METHODS The isolated bacteria were(identified) with API identified test(API Inc,France) and Kirby-Bauer(K-B) test used for the antibiotics(susceptivity) test.The data were analyzed by using WHONET-5 software.RESULTS Totally 604 strains of non-fermenting bacteria were isolated from the 2908 pathogenic strains.The most common bacteria were Pseudomonas aeruginosa(52.32%),followed by Stenotrophomonas maltophilia(14.07%) and Acinetobacter baumannii((13.74%)).76.32% of non-fermenting bacteria were isolated from the sputum.These bacteria had various(resistances) to all detected antibiotics.CONCLUSIONS Non-fermenting bacteria have high isolation rate and(multi-drug) resistance,so antibiotics should be used correctly under the guidance of antibiotic susceptibility testing.

OBJECTIVE A new piezoelectric aptamer biosensor is developed for determination of IgE. The energy converters are 10MHz AT-cut quartz crystals with gold-coated electrodes. The anti-IgE aptamers are immobilized onto the surfaces of crystals gold electrodes by biotin-avidin method. METHODS The standard substance and serum were detected to find the limit of detection and specificity of the biosensor. RESULTS The piezoelectric immunoglobulin aptamer biosensor could complete the detection without nonspecific response. Under the optimized conditions, the experimental results showed that the piezoelectric biosensor had good response to IgE whose frequency shifts were linearly dependent on IgE concentration in different range. The piezoelectric aptamer biosensor had been used to detect IgE in serum, the analytical results given by this method were in satisfactory agreement with those given by chemoluminescence method, its correlation coefficient was 0.9924. CONCLUSIONS Piezoelectric aptamer biosensor for the determination of IgE is of high sensitivity, high specificity, high analysis speed, unnecessary labeling, simple operation, real-time detection, etc. It is suitable for detection of IgE and should be used for clinical detection.