Objective To study on diagnosis and treatment of brain type fat embolism syndrome complicated with fracture. Method The diagnosis and treatment of 7 cases of brain type fat embolism syndrome complicated with fracture were reviewed. All of 7 cases did not have any brain injury, 3 cases were complicated with femural fracture, 3 cases with fractures of tibia and fibula, and 1 cases with limbs multi fracture. They presented somnolence or coma complicated with rise in temperature (37.7℃～39℃) ,increase in breath and heart rate,anemia,small bleeding spots at chest. It was discussed how to distinguish brain type fat embolism syndrome from brain injury. Synthetical measure was adopted mainly using dexamethasone (20～80mg/d). Results 7 cases of brain type fat embolism syndrome complicated with fracture were cured in 1～7 days. Conclusions Sudden somnolence or coma that does not result from brain injure after limb fracturees in early stage can be diagnosed as brain type fat embolism syndrome. A synthetical measure should be adopted by mainly using dexamethasone (40～80mg/d) .

Objective To analyze triple receptor-negative breast cancer (TNBC) and non-triple receptor-negative breast cancer (non-TNBC),to determine the MR imaging characteristics of TNBC.Methods Data from two groups TNBC and non-TNBC patients were reviewed.Recorded the MRI features which involved the morphological features,kinetic lesion features and measured ADC values,As well as their clinical features and the pathological findings.Data were statistical analyzed in SPSS 17.0 software.Results There were significant difference between TNBC and non-TNBC in histological types,histologic grade,the situation of lymph metastases and mass shape,margin subgroup and lesion enhanced type,the ADC value (P ＜ 0.05).The special histological types for TNBC were squamous carcinoma (metaplastic carcinomas),medullary carcinoma,basal-like carcinoma.The number of lymph node metastasis in the TNBC group was 78.9 ％ (15/19).Histologically,TNBC were mainly Ⅲ grade (89.4 ％).In the group of TNBC,the percent of lobulated mass,margin smooth and rim enhancement was 52.6 ％,36.8 ％,42.1％,the higher ADC value of TNBC than non-TNBC.While there were no statistical significant differences in the age of onset,the maximum tumor diameter,the situation of menstruation,the situation of family history of breast cancer,the number of the mass,enhancement lesion type and the time-signal intensity curve type (P ＞ 0.05).Conclusion Some MRI features combined clinical and histopathological characteristics would be helpful in the diagnosis of TNBC.

Aged ; Anthracosis ; complications ; Factor Analysis, Statistical ; Humans ; Middle Aged ; Pulmonary Disease, Chronic Obstructive ; complications

Aged ; Humans ; Lung Diseases, Fungal ; complications ; Male ; Pneumoconiosis ; complications ; microbiology

Animals ; Breast Neoplasms ; diagnosis ; genetics ; DNA, Mitochondrial ; genetics ; Electron Transport Complex IV ; genetics ; Humans ; Loss of Heterozygosity ; Microsatellite Instability ; Mitochondrial Proteins ; genetics ; Mutation

Objective To construct expressing recombinant of recF and recO from Deinococcus radiodurans R1and express the target protein in E.coli BL21(DE)3.Methods recF and recO gene were amplified by PCR from genomic DNA of Deinococcus radiodurans R1 and inserted into expression plasmid vector pET30b(+) to construct pET30b(+)-recF and pET30b(+)-recO.The recombinant plasmids were transformed into E.coli BL21(DE)3 and the recombinant proteins were expressed by the isopropyl-?-D-thiogalactopyranoside(IPTG) and were analyzed with SDS-PAGE.The changes in the survival rate of bacteria in each group before-and after UV-irradiation were calculated.Results The recombinant plasmid pET30b(+)-recO and pET30b(+)-recF was obtained and the recombinant protein could be highly expressed in E.coli BL21(DE)3.Conclusion This study has provided a foundation for further studies and applications of the recombinant RecF and RecO,and initial detection shows that recO and recF gene can increase the resistant ability of E.coli.

Objective To observe the clinical effect of the Placenta Polypeptides injection combined with paclitaxel plus paraplatin(TC)chemotherapy in the treatment of advanced ovarian cancer.Methods Eighty patients with advanced ovarian cancer receiving tumor cytoreductive surgery were divided into the treatment group and control group,40 cases in each group.The control group accepted single TC chemotherapy,while on this basis the treatment group was added with the Placenta Polypeptides injection.The short-term effect,KPS scores and occurrence of chemotherapeutic toxic reactions were observed in both groups.Results The short-term effective rates in the treatment group and control group were 90.00％ and 87.50 ％ respectively,the difference was not statistically significant (P＞0.05);the living quality KPS score in the treatment group was significantly better than that in the control group,the difference indicated statistical significance(P＜0.05).The occurrence rates of adverse reactions,including leukopenia,nausea and vomiting,liver function damage,heart toxicity,muscle and joint pain were significantly lower than those in the control group (P＜0.05).Conclusion The Placenta Polypeptides injection combined with TC chemotherapeutic regimen in the treatment of advanced ovarian cancer could improve the quality of life of the patients,reduce the toxic and adverse reactions of chemotherapeutic drugs.

Objective To observe the effect of mirror therapy and sensory reeducation on sensory function recovery after thumb recon-struction with second toe transplantation. Methods Forty patients after thumb reconstruction with second toe transplantation from January, 2011 to January, 2014 were divided into control group (n=20) and training group (n=20). Both groups accepted routine nursing and rehabili-tation, while the training group accepted mirror therapy and sensory reeducation training in addition. They were followed up for six to eigh-teen months after operation (average 9.3 months) with the two-point discrimination (2-PD) of transplanted toe pulp. Results 2-PD was (7.15±1.15) mm in the training group, very shorter than (10.23±2.23) mm of the control group (t=5.4898, P<0.001). Conclusion The mirror therapy combined with sensory reeducation training can improve the sensory function of the finger pulp that transplanted from the second toe.

Objective To explore the clinical nursing methods for the treatment of the skin degloving injury in foot by the combination of free femoral anterolateral skin flap transplantation and vacuum sealing drainage (VSD) technique. Methods From January 2010 to January 2014, 18 cases of the skin degloving injury in foot were treated by debridement and implant skin, negative pressure drainage and irrigation at first period, then the operation to repair the foot wound by transplant femoral anterolateral skin flap were done at second period. Nurses applied the key technology of VSD into postoperative nursing. Nurses should master the observation method and characteristics for the flow of blood to the flap skin, the method for foot position nursing, afford effective analgesic care, and flap local high flow oxygen therapy,improve the local tissue oxygen partial pressure and introduce the foot early functional exercise during postoperative. Results Eighteen cases flaps all survived. Clients were followed-up for 6 to 12 months,the discovery showed that all of the clients had good flap blood supply,the skin was soft and elastic,and the function of foot were recovered well. Conclusions The combination of of free femoral anterolateral skin flap transplantation and VSD technique apply into foot degloving injury repairation postoperative care,by application of high quality nursing, it really can improve the success rate of surgery and reduce the pain for clients.

Objective To analyze the causes of peripherally inserted central catheter (PICC) relat-ed infections, study the types of pathogens and their drug sensitivities in order to supply evidence for clini-cal medication. Methods 110 cancer patients were analyzed for PICC related infections and blood sam-pies underwent bacterial culture. Results Of 110 cases of cancer patients, catheter-related infections were found in 11 patients (10.00%), including 9 cases of local infection (8.18%), and 2 cases of hemato-ge-nous infection (1.82%). Catheter-related infections were not associated with gender, age, types of catheter, venous selection, and the inserted length of intravenous catheter. Catheter- related infections were likely to be associated with the indwelling time of catheters. Conclusions To avoid the infections related to long-term PICC, strict aseptic operation and standardized maintaining procedures are required.