OBJECTIVES: A salmonellosis outbreak occurred within a community of Gyeongju residents who ingested catered food from a wedding in June 2009. We aimed to epidemiologically investigate the probable vehicle of the infection. METHODS: We conducted a retrospective cohort study on 34 local residents who ingested the wedding food. RESULTS: Among the 34 residents, 31 (91.2%) reported symptoms of infection after eating the food. Among all of the wedding foods, pan-fried foods were highly associated with the diarrheal attack rate. On bacteriological examination, Salmonella species were detected in the pan-fried foods among the leftover foods and in 17 of the 31 stool specimens from the cases. There were five different types of pan-fried foods, but the onset of symptoms was independent of the ingredients used. We found that the pan-fried food was prepared at a food store in Seoul and that eggs were a common ingredient. CONCLUSIONS: The major cause of the salmonellosis in this population was presumed to be the pan-fried food prepared with contaminated eggs. These food items might have been partially undercooked because of their irregular shape, which allowed the Salmonella species to survive and multiply before ingestion.
Cohort Studies ; *Disease Outbreaks ; Eggs/microbiology ; Feces/microbiology ; *Food Microbiology ; Fruit/microbiology ; Humans ; Republic of Korea/epidemiology ; Retrospective Studies ; Salmonella/*isolation & purification ; Salmonella Food Poisoning/*epidemiology ; Time Factors

In recent years, China has become an increasingly important and the largest chestnut producer in the world. This study aimed to evaluate the nutritional value and microbiological quality of the roasted freeze-dried Chinese chestnut (Castanea mollissima) (RFDC) coated with dark chocolate (DCC) and milk chocolate (MCC) for industrial use and commercial consumption. Chocolate coating significantly improved the nutritional value of chestnut. RFDC had high levels of starch (66.23%) and fibers (3.85%) while DCC and MCC contained significantly high amounts of sucrose, protein, fat and minerals. Furthermore, the protein content doubled in MCC rather than in DCC. This could be attributed to the different formulations in the two products. Milk powder and whey protein constituted the source of protein in MCC while cocoa powder added to MCC formulation constituted an additional source of minerals. The amino acid profile showed differences in amino acid composition related to the sample's protein content, indicating their good nutritional quality. The moisture contents in all RFDC, DCC and MCC were suitable for industrial processing. These results provide information about the additional nutrients of chocolate-coated chestnut and confirm that the product is an interesting nutritional food. The combination of freeze-drying and chocolate-coating generally results in greater reductions on microbiological loads, extending shelf life of harvested chestnut for commercial application. This is an alternative strategy to add value to chestnut, minimizing the significant losses in harvested fruits and providing a wider range of choices of new products to the consumer disposal.
Cacao ; chemistry ; microbiology ; China ; Fagaceae ; chemistry ; microbiology ; Food Analysis ; Food Handling ; methods ; Food Microbiology ; Food Packaging ; methods ; Fruit ; chemistry ; microbiology ; Nutritive Value

AIMTo study the biotransformation of kaempferitrin, a major chemical principle of the fruits of Siraitia grosvenori (Swingle) C. Jeffery, with human intestinal flora.

METHODSThe kaempferitrin was incubated with human intestinal flora. The biotransformation products were isolated and purified by chromatographic methods and the structures were determined by spectroscopic techniques.

RESULTSKaempferitrin was converted into kaempferol 3-O-alpha-L-rhamnoside (afzelin, I) , kaempferol 7-O-alpha-L-rhamnoside (II), kaempferol (III) and p-hydroxybenzoic acid (IV) by human intestinal flora. rhamnoside (II), kaempferol (III) and p-hydroxybenzoic acid (IV) by human intestinal flora.

CONCLUSIONThe structure of kaempferitrin can be biotransformatedly converted by human intestinal flora.

Bacteria ; metabolism ; Biotransformation ; Fruit ; chemistry ; Humans ; Intestines ; microbiology ; Kaempferols ; isolation & purification ; metabolism ; pharmacokinetics ; Mannosides ; metabolism ; Momordica ; chemistry ; Parabens ; metabolism ; Plants, Medicinal ; chemistry ; Proanthocyanidins ; metabolism

Peach brown rot, caused by Monilinia fructicola, is one of the most serious peach diseases. A strain belonging to the Actinomycetales, named Streptomyces blastmyceticus JZB130180, was found to have a strong inhibitory effect on M. fructicola in confrontation culture. Following the inoculation of peaches in vitro, it was revealed that the fermentation broth of S. blastmyceticus JZB130180 had a significant inhibitory effect on disease development by M. fructicola. The fermentation broth of S. blastmyceticus JZB130180 had an EC₅₀ (concentration for 50% of maximal effect) of 38.3 µg/mL against M. fructicola, as determined in an indoor toxicity test. Analysis of the physicochemical properties of the fermentation broth revealed that it was tolerant of acid and alkaline conditions, temperature, and ultraviolet radiation. In addition, chitinase, cellulase, and protease were also found to be secreted by the strain. The results of this study suggest that S. blastmyceticus JZB130180 may be used for the biocontrol of peach brown rot.
Ascomycota/pathogenicity* ; Bacterial Proteins/metabolism* ; Cell Wall/metabolism* ; Cellulase/metabolism* ; Chitinases/metabolism* ; Fermentation ; Fruit/microbiology* ; Pest Control, Biological/methods* ; Phylogeny ; Plant Diseases/prevention & control* ; Prunus persica/microbiology* ; Siderophores/metabolism* ; Streptomyces/physiology*