1.Effects of top pruning on fruiting characters of Platycodon grandiflorum.
Zhi-fen WANG ; Cheng-gang SHAN ; Xue-he SU ; Shu-lin YAN ; Lian-xian ZHU ; Chun-qing SUI
China Journal of Chinese Materia Medica 2008;33(15):1807-1809
OBJECTIVETo investigate the effects of top pruning on fruiting characters of Platycodon grandiflorum, and find the suitable stage, in which seed growth and development furtherly.
METHODOne-year old seedlings were chosen and planted in field. Plant height, branching number, fruit number per plant, 1000 grains weight were measured during growth and development period, respectively.
RESULTThe treatment of top pruning postponed in turn the flowering date, lowered the plant heights and the fruit number per plant, increased the branching number and influenced significantly on 1000 grains weights.
CONCLUSIONThe suitable stage of top pruning for producing seeds was from June 20th to July 5th.
Crops, Agricultural ; growth & development ; Fruit ; growth & development ; Platycodon ; growth & development
2.Pollen vigor and development of germplasm of Dendrobium officinale.
Bo ZHU ; He YUAN ; Qiaoxian YU ; Jinping SI
China Journal of Chinese Materia Medica 2011;36(6):755-757
OBJECTIVETo provide the theoretical basis and applied technology for breeding superior species of Dendrobium officinale.
METHODThe peroxidase solution was used to test the pollen vigor in different flowering time and storage conditions. Cross, self and opening pollination were conducted in the green house, the subsidiary pollination by insects was carried out outdoors.
RESULTThe pollen of D. officinale was still in vigor when the flower faded. The pollen vigor was only 29.4% in the buds, 70.6% in the bloom day, and decreased to 31.9% a week later, it remained still 21.2% 20 days later under the condition of dry and 4 degrees C. The fructification rate was about 82.6% and 7.3%, respectively, when the cross and self pollination performed by hand in the whole flowering time, the rate was 0 in the green house and outdoors.
CONCLUSIONThe pollen of D. officinale was still in vigor during the whole flowering time, the fructification rate was 0 in the green house and outdoors resulted from the specific structure of flower. The wild resources of D. officinale were protected and germplasm was developed effectively through the artificial cross pollination.
Dendrobium ; growth & development ; Fruit ; growth & development ; Pollen ; physiology ; Pollination
3.Experiment on pruning of Cistanche deserticola inoculated in artificial Haloxylon ammodendron forest.
Bing-wen LI ; Zhong-liang ZHANG ; Gui-fen WANG ; Jing WANG ; Yong-zhi QIU ; Sheng-yu LI ; Zheng-zhong JIN ; Cong-juan LI ; Bo XU
China Journal of Chinese Materia Medica 2015;40(6):1086-1090
At present, the objective of cutting and pruning Cistanche deserticola is to harvest in successive years and enhance the harvesting yield and quality of C. deserticola in the process of the artificial cultivating C. deserticola. An experiment was conducted focusing on cutting and pruning C. deserticola in artificial forests of Haloxylon ammodendron drip-irrigated with saline water at the hinter-land of the Taklimakan desert, according to different growth stages and lengths. The results were following: (1) The effect of cutting on C. deserticola was similar to that of pruning, which resulted in three kinds of morphological types, not related to the bloom and size of C. deserticola. (2) The growth forms were diversified after pruning. Among them, there had sprouting new body, died or maintaining life with no sprouting, mildewed on its surface layer, etc. However, some of new bodies were sprouting from the lower part of the old body. The death rate of bloomed C. deserticola was higher than that of the underground, and the death rate of the 40 cm in stubble height for C. deserticola was higher than those with the stubble height of 20 cm and 5 cm. (3) Most of the diameter of living C. deserticola after pruning was increasing, but some of them changed little. (4) The mildew and rot of C. deserticola and the broken of the roots of the H. ammodendron and the fallen of the point of the inoculated when it was dug, which would cause the death of the C. deserticola. On the other, the yield-increasing effect and the economic benefit of the techniques of the pruning of Cistanche would need further research and evaluate. Therefore, the application of this technique needs to be cautious.
Amaranthaceae
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growth & development
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Cistanche
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growth & development
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Forests
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Fruit
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growth & development
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Plant Roots
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growth & development
4.Study on original plant species and geographical distribution of Fructus Aurantii.
Wei-Liang ZHAO ; Zeng-Xi GUO ; Wen-Ting ZHANG ; Qin-Wei HUANG ; Ze YI ; Jian-Feng SONG
China Journal of Chinese Materia Medica 2018;43(21):4361-4364
The original plant species of Fructus Aurantii are multitudinous and complex, and their requirements to the growing environment is strict. In order to clarify the original plant species and geographical distribution of Fructus Aurantii which recorded in the standards and circulated, used in commodity. The national and local standards of Chinese medicinal materials were collected and the original plants of Fructus Aurantii recoded in standards were found. Ten original plant species of Fructus Aurantii (including varieties of cultivars, the same below) were recorded in the Chinese pharmacopoeia and six local standards of Zhejiang, Yunnan and Guizhou etc. The producing areas and commodity in markets of Fructus Aurantii were investigated. The growth environment and geographical distribution of them were analyzed. There are six types of Fructus Aurantii i.e., Fructus Aurantii Chuan, Fructus Aurantii Xiang, Fructus Aurantii Jiang, Fructus Aurantii Qu, Fructus Aurantii Su, Fructus Aurantii Wen, and nineteen species of original plants in the practical commodities. There are four major Fructus Aurantii producing areas: Sichuan Basin, Dongting Lake Plain, Poyang Lake Plain, Jinqu Basin and its surrounding hilly areas. All of them are located in the area of the east longitude 104° to 121° and the northern latitudes 27° to 31°. There is a certain difference between the actual commodity and the standards of medicinal materials. It is suggested that the traditional mainstream types of Fructus Aurantii with fine quality should be accepted into Chinese Pharmacopoeia, and the types with poor quality should be withdrawn from Chinese Pharmacopoeia.
China
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Citrus
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classification
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growth & development
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Drugs, Chinese Herbal
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Fruit
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Geography
5.Growth curve of Siraitia grosvenorii and correlative analysis of seed and growth of fruit.
Lingyun WAN ; Xiaojun MA ; Jiaye LAI ; Changming MO ; Shixin FENG ; Hong LUO
China Journal of Chinese Materia Medica 2011;36(3):272-275
OBJECTIVETo establish the growth curve of Siraitia grosvenorii and analyze the correlation between seed and growth of fruit.
METHODThe growth curve fitting function was applied for the study of correlation between seed and fruit growth.
RESULTThe significant positive correlation existed between seed and horizontal diameter x vertical diameter, not between seed and flesh weight of single fruit.
CONCLUSIONThe growth curve is a reciprocal function, and seed is one of major factors to influencing the size of fruits and the shape of fruit.
Cucurbitaceae ; growth & development ; Fruit ; growth & development ; Seeds ; growth & development ; Time Factors
6.Growth and development of fruit and seed of Panax quinquefolium.
Xiaolei DAI ; Xian'en LI ; Qiaosheng GUO
China Journal of Chinese Materia Medica 2012;37(15):2272-2275
OBJECTIVETo understand the embryo after-ripening phenomenon of Panax quinquefolium, the growth and development process of fruit and seed was investigated in this study.
METHODThe growth and development characteristics of fruit and seed were obtained by field observation, the morphological changes were measured with a vernier caliper, paraffin section was used as well.
RESULT AND CONCLUSIONThe plant reached the most flowering numbers in 5-8 d after initial blooming time and the lag phase of embryo occurred in about 70 d. The size, fresh and dry weight of fruit and seed were all reached maximum in fruit maturation period. As the result showed, the development of seed was stopped at torpedo form of embryos, this conclusion can be applied to explore the morphological after-ripening mechanism of P. quinquefolium seed.
Fruit ; anatomy & histology ; growth & development ; Panax ; anatomy & histology ; growth & development ; Seeds ; anatomy & histology ; growth & development
7.Dissection of seed development of Gastrodia elata at different temperatures.
Qing-Song YUAN ; Hui WANG ; Wei-Ke JIANG ; Xiao-Hong OU ; Jiao XU ; Xiao-Ai WANG ; Lu WANG ; Yue-Yun OU ; Tao ZHOU
China Journal of Chinese Materia Medica 2020;45(3):485-490
The study is aimed to create seed materials and dissect the molecular mechanism of sexual propagation of Gastrodia elata. In this research, thirteen characteristics of flowers, flower stem, fruits, seeds and embryo of G.elata f. glauca and G.elata f. elata after bolting at room temperature(RT) and constant temperature(CT, 22 ℃) were determined. It was found that the constant temperature condition could prolong the bolting duration of G.elata and increased the number of flowers, while the variety of G.elata only affected the bolting duration, but had no effect on the number of flowers, and the G.elata f. elata was more likely to bolting than the G.elata f. glauca. The variety of G.elata was the main factor affecting the time of dehiscent fruit of G.elata, the temperature was the main factor affecting the fruits number and fruits diameter, and the constant temperature was more conducive to the fruits shape of G.elata than the room temperature. There was no significant difference in seed phenotype of G.elata varieties, but the seed embryo of G.elata seeds cultivated at constant temperature was fuller than that of G.elata cultivated at room temperature, and temperature had less influence on the seed phenotype of G.elata. But it was interesting to find that temperature and varieties had greater influence on the seed embryo of G.elata, constant temperature cultivation was more conducive to the formation of seed embryo of G.elata, and more the seed embryo of G.elata f. elata was easier to form than the seed embryo of G.elata f. glauca. However, the development of seeds and embryos of G.elata was significantly affected, and the development of seeds and embryos of G.elata f. glauca was more sensitive to temperature than G.elata f. elata. The research suggested that it is advisable for G.elata to produce seed materials by bolting at constant temperature(22 ℃).
Fruit/growth & development*
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Gastrodia/growth & development*
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Phenotype
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Reproduction
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Seeds/growth & development*
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Temperature
8.Effects of different harvest times and processing methods on quality of Gardeniae Fructus.
Tao LIU ; Xiaoheng GUO ; Yuling XU ; Deguang WAN
China Journal of Chinese Materia Medica 2012;37(13):1932-1934
OBJECTIVETo study effects of different harvest times and processing methods on the quality of Gardeniae Fructus.
METHODThe content of asminoidin and the similarity of the fingerprint of Gardeniae Fructus were determined and applied for assessment of the quality.
RESULTGardeniae Fructus harvested in October with yellow-green appearance was the better time point for harvest. Drying in the sun and boiling in the water is the better processing method for Gardeniae Fructus.
CONCLUSIONDifferent harvest times and processing methods can affect the quality of Gardeniae Fructus.
Drugs, Chinese Herbal ; chemistry ; standards ; Fruit ; chemistry ; growth & development ; Gardenia ; chemistry ; growth & development ; Quality Control
9.Study on exogenous hormones inducing parthenocarpy fruit growth and development and quality of Siraitia grosvenorii.
Jie HUANG ; Dong-ping TU ; Xiao-jun MA ; Chang-ming MO ; Li-mei PAN ; Long-hua BAI ; Shi-xin FENG
China Journal of Chinese Materia Medica 2015;40(18):3567-3572
To explore the growth and development and analyze the quality of the parthenocarpy fruit induced by exogenous hormones of Siraitia grosvenorii. the horizontal and vertical diameter, volume of the fruit were respectively measured by morphological and the content of endogenous hormones were determined by ELISA. The size and seed and content of mogrosides of mature fruit were determined. The results showed that the fruit of parthenocarpy was seedless and its growth and development is similar to the diploid fruit by hand pollination and triploid fruit by hand pollination or hormones. But the absolute value of horizontal and vertical diameter, volume of parthenocarpy fruit was less than those of fruit by hand pollination, while triploid was opposite. The content of IAA, ABA and ratio of ABA/GA was obviously wavy. At 0-30 d the content of IAA and ABA of parthenocarpy fruit first reduced then increased, content of IAA and GA parthenocarpy fruit was higher than that of fruit by hand pollination. Mogrosides of parthenocarpy fruit was close to pollination fruit. Hormones can induce S. grosvenorii parthenocarpy to get seedless fruit and the fruit shape and size and quality is close to normal diploid fruit by hand pollination and better than triploid fruit by hormone or hand pollination.
Cucurbitaceae
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chemistry
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drug effects
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genetics
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growth & development
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Diploidy
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Fruit
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chemistry
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genetics
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growth & development
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Plant Growth Regulators
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pharmacology
10.Pollen viability, stigma receptivity and fruiting characteristics of botanical origin of Jinxianlian.
Qing-song SHAO ; Yong WANG ; Run-huai HU ; Wang-shu ZHANG ; Ming-yan LI
China Journal of Chinese Materia Medica 2015;40(6):1061-1065
The viability and life span of pollen were evaluated by TTC (2,3,5-triphenyl tetrazlium chloride) and the peroxidase solution, the stigma receptivity were estimated by benzidine-H2O2 method and the fruiting characteristics were investigated. The results showed that (1) Anoectochilus roxburghii and A. formosanus appeared the same up-and-down trend of the pollen viability, increased and then decreased. The storage temperature and storage time had significant impact on the pollen viability. With the extension of storage time, the pollen activity decreased. 4 degrees C refrigerator storage may be extended the pollen vitality. (2) The stigma had receptivity in 1st day and reached the highest level in the 4th day after blooming. A. roxburghii lost receptivity in the 8th day while A. formosanus lost receptivity in the 10th day after blooming. (3) The different pollination had significant impact on seed setting rate. The seed setting rate of artificial cross-pollination was higher than that of the artificial self-pollination. Collecting pollen in the 3rd day and carrying out artificial cross-pollination in the 4th day after blooming can significantly improve seed setting rate. The results provided technical assurance for A. roxburghii and A. formosanus breeding of new varieties and seed breeding.
Cell Survival
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China
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Flowers
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physiology
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Fruit
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growth & development
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Orchidaceae
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growth & development
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physiology
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Pollen
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growth & development
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Reproduction
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Temperature