1.Protective and Anti-Pathology Effects of Sm Fructose-1,6-Bisphosphate Aldolase-Based DNA Vaccine against Schistosoma mansoni by Changing Route of Injection.
Mohamed SABER ; Tarek DIAB ; Olft HAMMAM ; Amr KARIM ; Amina MEDHAT ; Mamdouh KHELA ; Ehab EL-DABAA
The Korean Journal of Parasitology 2013;51(2):155-163
This study aimed to evaluate the efficacy of fructose-1,6-bis phosphate aldolase (SMALDO) DNA vaccination against Schistosoma mansoni infection using different routes of injection. The SMALDO has been cloned into the eukaryotic expression vector pcDNA3.1/V5-His TOPO-TA and was used in injecting Swiss albino mice intramuscularly (IM), subcutaneously (SC), or intraperitoneally (IP) (50 microg/mouse). Mice vaccinated with non-recombinant pcDNA3.1 served as controls. Each group was immunized 4 times at weeks 0, 2, 4, and 6. Two weeks after the last booster dose, all mice groups were infected with 80 S. mansoni cercariae via tail immersion. At week 8 post-infection, animals were sacrificed for assessment of parasitological and histopathological parameters. High anti-SMALDO IgG antibody titers were detected in sera of all vaccinated groups (P<0.01) compared to the control group. Both the IP and SC vaccination routes resulted in a significant reduction in worm burden (46.2% and 28.9%, respectively, P<0.01). This was accompanied by a significant reduction in hepatic and intestinal egg counts (41.7% and 40.2%, respectively, P<0.01) in the IP group only. The number of dead eggs was significantly increased in both IP and IM groups (P<0.01). IP vaccination recorded the highest significant reduction in granuloma number and diameter (54.7% and 29.2%, respectively, P<0.01) and significant increase in dead miracidia (P<0.01). In conclusion, changing the injection route of SMALDO DNA vaccination significantly influenced the efficacy of vaccination. SMALDO DNA vaccination via IP route could be a promising protective and anti-pathology vaccine candidate against S. mansoni infection.
Animals
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Antibodies, Helminth/blood
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Disease Models, Animal
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Female
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Fructose-Bisphosphate Aldolase/genetics/*immunology
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Histocytochemistry
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Immunoglobulin G/blood
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Injections, Intramuscular
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Injections, Intraperitoneal
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Injections, Subcutaneous
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Mice
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Parasite Load
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Schistosoma mansoni/enzymology/genetics/*immunology
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Schistosomiasis mansoni/immunology/parasitology/pathology/*prevention & control
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Vaccination/methods
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Vaccines, DNA/administration & dosage/genetics/*immunology
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Vaccines, Synthetic/administration & dosage/genetics/immunology