1.The chracteration analy of HAV recombinant antigen was expressed by vaccinia virus vector
Hong-Lan ZHAO ; Fong QIOU ; Qing-Ling MENG ; Yao YI ; Rui-Guang TIAN ; Jian LU ; Jing-Yuan CAO ; Sheng-Li BI
Chinese Journal of Experimental and Clinical Virology 2011;25(6):450-452
Objective To find a suitable cell line for hepatitis A antigen expressed by vaccinia virus vector and to find a way of inactivation and preservation of the HAV recombinant antigen.Methods Series of cell lines such as K4,143,HEL,Hep-2 and Vero were inoculated with vaccinia virus that can express HAV recombinant antigen.ELISA was used to determine the contents of expression antigen.The chracteration of the HAV antigen expressed by vaccinia virus was then analyzed after it was treated with different methods.Results The expression of HAV recombinant antigen in K4,143 and HEL cell lines was a little more than expression in Hep-2 and Vero cell lines.The antigenicity is obviously higher when HAV recombinant antigen was inactivated by β-propiolactone other than it was inactivated by formalin.It was best to preserve the prepared HAV recombinant antigen under -40℃ condition.Conclusions The application of vaccinia virus vector in hepatitis A antigen preparation was very useful and promising.
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