1.Study on follicular fluid steroid concentration according to success of in vitro fertilization.
Hyung Bae PARK ; Kyung Sook CHO ; Chung Soon BACK ; Jae Myung KIM ; Sun Kyung LEE ; Byung Hee SHU ; Jae Hyun LEE
Korean Journal of Obstetrics and Gynecology 1991;34(4):537-553
No abstract available.
Female
;
Fertilization in Vitro*
;
Follicular Fluid*
2.correlation between the concentrations of follicular fluid epidermal growth factor and estradiol in IVF program.
Hyeong Soon KIM ; Sung Sik HAN ; Kyung Hwan CHANG ; In Cheol CHOI ; Won Il PARK ; Dong Jae CHO ; Chan Ho SONG
Korean Journal of Fertility and Sterility 1993;20(3):209-216
No abstract available.
Epidermal Growth Factor*
;
Estradiol*
;
Female
;
Follicular Fluid*
3.Correlation between fertilization rate and human follicular fluid prostaglandin E2, prostaglandin F2a, prostaglandin E2: prostaglandin F2a ratio.
Jung Pil LEE ; Kyung Hwan CHANG ; Dong Jae CHO ; Ki Hyun PARK ; Won Il PARK ; Gyung Soo KIM ; Chan Ho SONG
Korean Journal of Fertility and Sterility 1993;20(3):201-208
No abstract available.
Dinoprostone*
;
Female
;
Fertilization*
;
Follicular Fluid*
;
Humans*
5.Prostaglandin F2 alpha levels of ovarian follicular and peritoneal fluid during preovulatory phase in the women with and without endometriosis.
Gyung Joon MIN ; Tae Jin YOON ; Sur Gyu SHIN ; Yong Bum KIM ; Jae Sook RHO ; Il Woon JEE ; Eun Hwan JUNG ; Hak Soon KIM
Korean Journal of Obstetrics and Gynecology 2000;43(11):1983-1989
OBJECTIVE: The aim was to evaluate whether the differences of PG concentration in follicular and peritoneal fluid during preovulatory phase exist between the women with and without endometriosis. MATERIAL AND METHODS: Twenty-three patients with endometriosis, 8 were stage I-II and 15 were stage III-IV, and another 23 patients without endometriosis were undergone laparotomy during late follicular phase. Peritoneal fluid from 46 patients and follicular fluid from 42 patients were obtained, and these samples were analyzed double times for PGF2alpha, PGE2 and estradiol. RESULTS: The mean level of PGF2alphain the peritoneal fluid was significantly higher in the group with endometriosis than in the control(P=0.0293), especially more significant in stage I-II endometriosis. Although there was no significant difference of PGF2alphaconcentration in the follicular fluid between the groups, the stage III-IV endometriosis group showed slightly higher PGF2alphalevel than both the stage I-II group and the control(P=0.0604). And also, there was significant positive correlation with the level of PGF2alphaand estradiol in the follicular fluid only in the endometriosis group(r=0.4988, P=0.0154), not in the control. However, there was no difference in the level of PGE2 and estradiol in the peritoneal or follicular fluid between the groups. CONCLUSION: Some alterations of PGF2alphalevel exist in the women with endometriosis. These are significantly higher PGF2alphalevel in peritoneal fluid with mild endometriosis and slightly higher PGF2alphalevels in follicular fluid with extensive endometriosis during preovulatory phase, which suggest that PGF2alphamay play some roles in subfertility associated with endometriosis.
Ascitic Fluid*
;
Dinoprost*
;
Dinoprostone
;
Endometriosis*
;
Estradiol
;
Female
;
Follicular Fluid
;
Follicular Phase*
;
Humans
;
Infertility
;
Laparotomy
6.Progress in the effect of microRNA carried by extracellular vesicles in follicular fluid on follicular atresia.
Ying WANG ; Xiaomei WANG ; Yunqi ZHAO ; Shenghui WU ; Yong ZHANG ; Fusheng QUAN
Chinese Journal of Biotechnology 2022;38(8):2767-2783
Extracellular vesicles (EVs) are membrane-bound particles actively released by cells. In prokaryotes and eukaryotes, EVs are effective bridges for communication between cells. EVs carry biological macromolecules, including proteins, lipids and nucleic acid, which affects different physiological functions of parent cells and recipient cells. Among them, the microRNA carried by EVs is the most reported and plays an important role in physiological function of organisms. During the development of follicles, only a few follicles can fully develop and ovulate, whereas most of them undergo atresia at different stages of development. In the whole process of follicular development, the changes at each stage and the regulation mechanism of follicular atresia are not completely understood. In this paper, we introduced the types, characteristics, isolation methods and uses of EVs, and emphasized how microRNA carried by EVs in follicular fluid regulated follicular atresia from the aspects of different cytokines and hormones. Additionally, the application prospect of microRNA carried by EVs in follicular fluid in reproductive regulation and reproductive disease diagnosis was discussed. This paper is significant for studying the regulation of follicular development and the effective utilization of oocytes.
Animals
;
Extracellular Vesicles/metabolism*
;
Female
;
Follicular Atresia
;
Follicular Fluid
;
MicroRNAs/metabolism*
;
Oocytes
7.Effect of mature human follicular fluid on the development of mouse embryos in vitro.
Sae Young PARK ; Jeong Jae LEE ; Sun Haeng KIM ; Pyong Sam KU
Korean Journal of Fertility and Sterility 1992;19(2):125-131
No abstract available.
Animals
;
Embryonic Structures*
;
Female
;
Follicular Fluid*
;
Humans*
;
Mice*
8.Comparison between Human Follicular Fluid and SpermGrad for Sperm Preparation in Asthenozoospermia.
Youn Kyung CHUNG ; Jung Ryeol LEE ; Jeong Hee MOON ; Hyun Jun KIM ; Sang Hoon HAN ; Byung Chul JEE ; Seung Yup KU ; Chang Suk SUH ; Seok Hyun KIM ; Young Min CHOI ; Jung Gu KIM ; Shin Yong MOON
Korean Journal of Fertility and Sterility 2006;33(1):53-60
OBJECTIVE: We tested the usefulness of swim-down technique using human follicular fluid (hFF) in sperm preparation. METHODS: Semen samples were obtained from twelve male partners showing asthenozoospermia (sperm motility < 50%) at the time of routine andrologic evaluation in Seoul National University Bundang Hospital. After dividing into two aliquots, each samples were processed either by swim-down using 100% hFF or density gradient using SpermGrad. Sperm quality was assessed by computer-assisted semen analyzer (CASA). RESULTS: Motility, Rapid motility, VCL (curvilinear velocity), ALH (amplitude of lateral head displacement), and hyperactivated sperms were significantly increased, and LIN (mean linearity) was decreased significantly after sperm preparation in both groups. Motility was significantly higher after swim-down using 100% hFF when compared with density gradient using SpermGrad (81.2+/-4.7 vs. 67.6+/-2.3, p=0.02). The other parameters assessed by CASA were not different between the two methods. CONCLUSION: Swim-down method with 100% hFF may be a useful method in preparation of sperm from asthenozoospermia.
Asthenozoospermia*
;
Female
;
Follicular Fluid*
;
Head
;
Humans*
;
Male
;
Semen
;
Seoul
;
Spermatozoa*
9.Ovarian follicular and peritoneal fluid prostaglandin E2 and F2a levels according to the clinical symptoms in the women with endometriosis.
Jong Cheol LEE ; Kyu Sang KYOUNG ; A Ra CHO ; Min Ah PARK ; Young Mi LEE ; Seung Hwa HONG ; Yeon Jin PARK ; Ill Woon JI ; Eun Hwan JEONG ; Hak Soon KIM
Korean Journal of Obstetrics and Gynecology 2007;50(8):1107-1114
OBJECTIVE: The purpose of the present study was to determine whether a relationship exists between the clinical symptoms (dysmenorrhea and infertility) and prostaglandin (PG) concentrations in follicular and peritoneal fluid in the women with endometriosis during the late follicular phase of the menstrual cycle. METHODS: Thirty patients with pelvic endometriosis diagnosed by pelvic surgery were enrolled. Eight patients were suffering from severe dysmenorrhea and 11 had history of primary or secondary infertility among them. Endometriosis patients were grouped by the presence of each symptom and compared with 33 control patients without endometriosis. Peritoneal fluid was collected in the beginning of peritoneal opening and dominant follicular fluid was aspirated by syringe needle at the time of operation. Then PGE2 and PGF2a concentration were measured at each tube. RESULTS: Follicular fluid PGF2a levels were increased in 30 endometriosis patients (P=0.003), and the levels were significantly higher in 11 patients with infertility compared with the control (P=0.001). Peritoneal fluid PGF2a levels were significantly higher in 8 patients with severe dysmenorrhea compared with the others or the control (P=0.028). Follicular or peritoneal fluid PGE2 levels were not different between any group and the control. There was no significant correlation between size of endometrioma and each PG levels. CONCLUSION: Severe dysmenorrhea in endometriosis patients would be related with the high level of peritoneal fluid PGF2a concentration, and infertility in endometriosis would be related with the high level of follicular fluid PGF2a concentration.
Ascitic Fluid*
;
Dinoprostone*
;
Dysmenorrhea
;
Endometriosis*
;
Female
;
Follicular Fluid
;
Follicular Phase
;
Humans
;
Infertility
;
Menstrual Cycle
;
Needles
;
Prostaglandins
;
Syringes
10.Effects of Age on in vitro Maturation and Fertilization of Immature Oocytes from Stimulated Cycles in Human IVF-ET Program.
Sang Hoon HAN ; Jung Ryeol LEE ; Hyun Jun KIM ; Jung Hee MOON ; Byung Chul JEE ; Seung Yup KU ; Chang Suk SUH ; Seok Hyun KIM ; Young Min CHOI ; Jung Gu KIM ; Shin Yong MOON
Korean Journal of Fertility and Sterility 2005;32(4):331-336
OBJECTIVE: To investigate the effects of female age on in vitro maturation and fertilization of immature oocytes from controlled ovarian hyperstimulation (COH) in human IVF-ET program. METHOD: A total of 96 immature oocytes (GV & metaphase I) obtained from 40 cycles of IVF-ET (29 patients). The mean age of female patients was 31.8+/-3.1 years. Ovulation was triggered by urinary or recombinant hCG. Immature oocytes were cultured with YS medium containing 30% of patients' human follicular fluids, LH (1 IU/mL), FSH (1 IU/mL) and EGF (10 ng/mL), and then matured oocytes were fertilized by ICSI. In vitro maturation and fertilization of immature oocytes were analyzed according to age of female (< 34 or > or = 34 years). RESULTS: The maturation rate was similar between two groups (68% vs 64%). The fertilization rate of in?vitro-matured oocytes was higher in patients < 34 years old, but there was no statistical significance (64% vs 50%, p=0.347). The fertilization rate of in-vitro-matured oocytes was significantly lower compared with those of in-vivo-matured oocytes in both age groups (64% vs 79%, p=0.035, 50% vs 86%, p=0.007). CONCLUSION: In older female group, fertilization rate of in-vitro-matured oocytes seems to be decreased. Further investigations should be warranted to increase fertilization potential of in-vitro-matured oocytes.
Adult
;
Epidermal Growth Factor
;
Female
;
Fertilization*
;
Follicular Fluid
;
Humans*
;
Metaphase
;
Oocytes*
;
Ovulation
;
Sperm Injections, Intracytoplasmic