1.Optical Nano-imaging for the Diagnosis of Gastrointestinal Cancers.
The Korean Journal of Gastroenterology 2007;49(5):287-293
Development of in vivo animal imaging instrumentations and methods contributes to the early diagnosis of cancer. Of variable imaging modalities, in vivo optical imaging such as bioluminescence and fluorescence is one of the best methods to measure molecular change of cancer cells. High sensitivity and relatively low cost of optical method gives benefits to apply for translational research in the field of cancer. Nano-probes to label and detect early cancer cells have been developed by nano-chemists and molecular imaging researchers. Quantum dots made from fluorescent semi-conductors show good advantages in term of imaging probes; high quantum yields, large molar extinction coefficients, size-dependent tunable emission and high photostability. To detect a gastrointestinal (GI) cancer, newly developed endoscopes have been used. Among them, near infrared fluorescence endoscope and confocal endomicroscope are good candidates for clinical application. In animal studies, successful results to detect cancer in gastrointestinal tract have been obtained. Prospect of nanoparticles as optical imaging moiety is promising to detect GI cancers if their toxicity is minimized. Future fluorescence confocal endoscope with safe cancer targeting nanoparticles will be useful for the detection and treatment of GI cancers.
Diagnostic Imaging
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Endoscopy, Gastrointestinal/*methods
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Fluorescent Dyes/*diagnostic use
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Gastrointestinal Neoplasms/*diagnosis
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Humans
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Molecular Diagnostic Techniques
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Nanoparticles/*diagnostic use
2.Fluorescent low density lipoprotein and acetylated low density lipoprotein labeling of cultured bovine trabecular endothelial cells.
Byung Heon AHN ; Myung Ju OH ; Hyun Bong BAE
Korean Journal of Ophthalmology 1987;1(2):90-94
A preliminary study was performed to investigate the staining characteristics of trabecula. endothelial cells with low density lipoprotein (LDL) and acetylated low density lipoprotein (Ac-LDL) labeled with a fluorescent probe, 1, 1`- dioctadecyl-3,3,3`, 3`- tetramethyl-indocarbocyanine perchlorate (Dil). Trabecular endothelial cells revealed a strong fluorescence with Dil-LDL, which was contradictory to the previous results obtained from other types of endothelial cells. These cells also showed a moderate fluorescence with Dil-Ac-LDL. Scleral fibroblasts and keratocytes showed a moderate to strong fluorescence with Dil-LDL and a weak fluorescence with Dil-Ac-LDL. Corneal endothelial cells revealed a very weak background fluorescence with Dil-LDL and a moderate fluorescence with Dil-Ar-LDL. Therefore, these four kinds of cells could not be definitely differentiated depending only on the staining characteristics with Dil-LDL and Dil-Ac-LDt.
Animals
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Cattle
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Endothelium/cytology
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Fluorescent Dyes/*diagnostic use
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Lipoproteins, LDL/*metabolism
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Trabecular Meshwork/*metabolism
3.Sentinel Lymph Node Mapping of the Stomach Using Fluorescent Magnetic Nanoparticles in Rabbits.
Jin Soo KIM ; Tae Jong YOON ; Hyung Keun KIM ; Sung Soo KIM ; Hiun Suk CHAE ; Myung Gyu CHOI ; Yong Jin KIM ; Gyu Chul YI ; Young Seok CHO
The Korean Journal of Gastroenterology 2008;51(1):19-24
BACKGROUND: Sentinel lymph node (SLN) mapping of the stomach cancer using available techniques is limited by unpredictable lymphatic drainage patterns and skip metastasis. The aim of this study was to test the feasibility of gastric SLN mapping using fluorescent magnetic nanoparticles (FMNP) of uniform nano-size. METHODS: Biocompatible silica-overcoated magnetic nanoparticles containing rhodamine B isothiocyanate (RITC) within a silica shell of controllable thickness with 60 nm thickness were used as model nanomaterials. Gastric lymphatic mapping was performed by injecting 100 microliter of either FMNP or isosulafan blue subserosally. Gastric injections (n=7) were made into the body, approximately 5 cm from the lesser curvature of rabbits. Sentinel lymph nodes were visualized using fluorescent nanoparticle detection system. RESULTS: In 7 rabbits, it was demonstrated that FMNP quickly and accurately detected sentinel lymph nodes. Injection into the stomach resulted in identification of a retrogastric lymph node. Histological analysis in all cases confirmed the presence of nodal tissue. CONCLUSIONS: FMNP can be a potential alternative to existing tracers in the detection of SLN in this animal experiment.
Animals
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Coloring Agents/diagnostic use
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Female
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Fluorescent Dyes/*diagnostic use
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Lymph Nodes/pathology
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Male
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Models, Animal
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Nanoparticles/*diagnostic use
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Rabbits
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Rhodamines/diagnostic use
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Rosaniline Dyes/diagnostic use
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Sentinel Lymph Node Biopsy/*methods
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Stomach/*pathology
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Time Factors
4.Current Status of Optical Imaging for Evaluating Lymph Nodes and Lymphatic System.
Eun Seong LEE ; Tae Sung KIM ; Seok Ki KIM
Korean Journal of Radiology 2015;16(1):21-31
Optical imaging techniques use visual and near infrared rays. Despite their considerably poor penetration depth, they are widely used due to their safe and intuitive properties and potential for intraoperative usage. Optical imaging techniques have been actively investigated for clinical imaging of lymph nodes and lymphatic system. This article summarizes a variety of optical tracers and techniques used for lymph node and lymphatic imaging, and reviews their clinical applications. Emerging new optical imaging techniques and their potential are also described.
Contrast Media/diagnostic use
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Fluorescent Dyes/diagnostic use
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Humans
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Lymph Nodes/pathology/*radiography
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Lymphatic System/*pathology
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Lymphography
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Magnetic Resonance Imaging
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*Optical Imaging
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Positron-Emission Tomography
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Quantum Dots/diagnostic use
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Spectroscopy, Near-Infrared
5.Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.
Alfredo GARCIA ; Remigio MARTINEZ ; Jose Manuel BENITEZ-MEDINA ; David RISCO ; Waldo Luis GARCIA ; Joaquin REY ; Juan Manuel ALONSO ; Javier Hermoso DE MENDOZA
Journal of Veterinary Science 2013;14(4):491-494
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
Actinomycetales/*isolation & purification
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Actinomycetales Infections/diagnosis/microbiology/*veterinary
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Animals
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Cattle
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Cattle Diseases/*diagnosis/microbiology
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Fluorescent Dyes/*diagnostic use
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Horse Diseases/*diagnosis/microbiology
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Horses
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Limit of Detection
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Real-Time Polymerase Chain Reaction/*methods/veterinary
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Reproducibility of Results
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Sheep
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Sheep Diseases/*diagnosis/microbiology