1.Whole blood cyclosporine measurement by fluorescence polarization immunoassay.
Chan Jung PARK ; Kyung Ryung KANG ; Hyun Chan CHO ; Kyung Hwa LEE ; Hong Rae CHO ; Young Joo LEE ; Yoo Sun KIM ; Eun Mi LEE ; Ki Il PARK
The Journal of the Korean Society for Transplantation 1993;7(1):77-82
No abstract available.
Cyclosporine*
;
Fluorescence Polarization Immunoassay*
;
Fluorescence Polarization*
;
Fluorescence*
2.The development of a fluorescence polarization immunoassay for aflatoxin detection.
Ya Jie SHENG ; Sergei EREMIN ; Tie Jun MI ; Su Xia ZHANG ; Jian Zhong SHEN ; Zhan Hui WANG
Biomedical and Environmental Sciences 2014;27(2):126-129
A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 min. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.
Aflatoxins
;
analysis
;
Fluorescence Polarization Immunoassay
3.Comparison of the Antibiotic Release Kinetics from the Implant Coated with Antibiotic-impregnated Polymers.
Young Wan MOON ; Youn Soo PARK ; Byung Taek LEE ; Soowon SUH ; Jeong Jun YU
Journal of Korean Orthopaedic Research Society 2004;7(2):178-183
PURPOSE: To compare the antibiotic release kinetics of the implant coated with antibiotic-impregnated polymers MATERIALS AND METHODS: Authors used polylactic acid (PLA) and polylactic-co-glycolic acid (PLGA) as the biodegradable carriers, gentamicin sulfate as the antibiotic and Steinmann pin as the implant. Ten Steinmann pins were coated with gentamicin of each 10, 20 and 30% mixture of PLA or PLGA for the elution kinetics study. In the elution study, total 60 coated implants were incubated in 10 mL of phosphate buffered saline (PBS) at 37 delta C and sampled at 6 hrs, 1, 3, 6, 9, 12, 15, 20, and 25 days. Assays were performed with fluorescence polarization immunoassay. Statistical analysis was done with SAS release 2.01. RESULTS: Released concentration of GM decreased with time. Minimum inhibitory concentration was maintained until 6th day on PLA 10% subgroup, 9th day in the 20 and 30% subgroups, until 6th day on PLGA 20% subgroup, and 3rd day in the 10 and 30% subgroups. Released concentrations were significantly higher in all PLA subgroups than in PLGA as a parameter of sampled time (all p<0.05). There was no statistical difference between PLA 20 and 30% subgroup after 12th sampled day (p=0.2636). CONCLUSION: PLA-GM group showed higher effective concentration for longer time than PLGA-GM group. 20 and 30% subgroups of PLA-GM showed prolonged maintenance of minimum inhibitory concentration compared with 10% subgroup, but there was no difference between the two groups.
Fluorescence Polarization Immunoassay
;
Gentamicins
;
Kinetics*
;
Microbial Sensitivity Tests
;
Polymers*
4.measurement of Percutaneous Absorption Using Fluorescence Polarization Immunoassays in a Diffusion Chamber.
Weon Ju LEE ; Yong Hyun KIM ; Do Won KIM ; Jae Bok JUN ; Sang Lip CHUNG
Korean Journal of Dermatology 1996;34(5):718-724
BACKGROUND: The penetraton in vivo of topically applied substances can be assessed by physiological or pharmacalogical signs or analysed by chemical or histological techniques. In vitro absorption can be commonly quantitated by measuring the passage of a radioisotope-labelled substance across skin that has been mounted in a diffusion chamber. OBJECTIVE: Fluorescence polarization immunoassay technique has made the possible rapid growth of therapeutic drug nonitoring. We applied this methodology in measuring percutaneous absorption in a diffusion chamber. METHODS: We utilized sheets of whole epidermis prepared from the circumcised prepuce. Some epidermal sheets were treated with 2 ml of acetone for 2 minutes, and others not. The epidermal sheet was mounted in a diffusion chamber between the donor compartment for the penetrant and the receptor compartment containing saline. Lidocaine HC1(10 microgram/cm2) in vehicle(propylene glycol:ethanol; 7:3, vol/vol) was applied to the donor compartment for the penetrant. With flow rate of about 3 ml/h all of the receptor phase collected during 2 hours interval were quantitated for 10 hours by the fluorescence polarization immunoassay. RESULTS: Total absorption of lidocaine HC1 in the acetone-untreated group was 2.14+/-0.74% of the applied dose. Total absorption in the acetone-treated group showed no substantial difference (2.09+/-1.25%) compared to those of acetone-untreated group. The amount of lipid extracted from a epiderrnal sheet with acetone was 19+/-2.97%. CONCLUSION: Fluorescence polarization immunoassay may be a useful method in measuring percutaneous absorption in vitro.
Absorption
;
Acetone
;
Diffusion*
;
Epidermis
;
Fluorescence Polarization Immunoassay*
;
Fluorescence Polarization*
;
Fluorescence*
;
Histological Techniques
;
Humans
;
Lidocaine
;
Skin
;
Skin Absorption*
;
Tissue Donors
5.Comparison of High Performance Liquid Chromatography and Fluorescence Polarization Immunoassay for Determination of Total Homocysteine in Human Plasma.
Moon Soo KANG ; Jong Weon CHOI ; Chung Hyun NAHM ; Jong Wook LEE ; Chul Hoon LEE ; Jin Ju KIM ; Soo Hwan PAI
Korean Journal of Clinical Pathology 1999;19(5):510-515
BACKGROUND: It was purposed to estimate correlation between fluorescence polarization immunoassay (FPIA) and high performance liquid chromatography (HPLC), and precision of individual methods. It was also objected to describe distribution of plasma total homocysteine in Korean adults. METHODS: The subjects were 100 adults admitted to Inha University Hospital during the month of October, 1998. The total plasma homocysteine concentration was measured by FPIA (IMx analyzer, Abbott Laboratories, IL, USA) and by HPLC (ACCLAIM Biogenic Amines Testing System, Bio-Rad Laboratories, CA, USA) using Bio-Rad Homocysteine. RESULTS: Plasma homocysteine levels (mean+/-SD) from Korean healthy adults by FPIA and HPLC were 9.75+/-3.80micromol/L, 7.72+/-3.36micromol/L, respectively. Plasma homocysteine levels according to sex by FPIA were 11.79micromol/L for male, 7.71micromol/L for female, and those by HPLC were 9.47micro mol/L for male, 5.98micromol/L for female, respectively. Intra-assay coefficient variations (CVs) of low, medium, and high concentration by FPIA are 1.83%, 0.47%, and 1.66%, and those by HPLC are 5.53%, 5.37%, and 4.56%, respectively. Inter-assay CVs of low, medium, and high concentration by FPIA are 2.28%, 1.44%, and 1.29%, and by HPLC are 7.23%, 5.54%, and 4.95%, respectively. CONCLUSION: Plasma homocysteine levels from male were significantly higher than female in Korean. Plasma homocysteine levels were increased according to increment of age. FPIA was more convenient, automatic, rapid, and reproducible than HPLC and also excellently correlated with HPLC. It is concluded that FPIA will potentially benefit for quantifying homocysteine in clinical laboratories.
Adult
;
Biogenic Amines
;
Chromatography, High Pressure Liquid
;
Chromatography, Liquid*
;
Female
;
Fluorescence Polarization Immunoassay*
;
Fluorescence Polarization*
;
Fluorescence*
;
Homocysteine*
;
Humans*
;
Male
;
Plasma*
6.Serum Homocysteine and Folate Levels in Korean Schizophrenic Patients.
Psychiatry Investigation 2011;8(2):134-140
OBJECTIVE: This study was conducted to confirm the results of the authors' previous research on schizophrenia manifesting high serum homocysteine and low folate levels. This study is anchored on a theory that a high serum homocysteine concentration affects schizophrenia by virtue of a neurotoxic mechanism, and on a report that some schizophrenia patients with high homocysteine levels benefited from high folate ingestion. METHODS: The serum homocysteine, folate, and vitamin B12 levels of 236 normal-control-group subjects and 234 schizophrenia subjects who met the diagnostic criteria based on DSM-IV-TR were compared. The homocysteine levels were measured via fluorescence polarization immunoassay, and the folate and vitamin B12 levels were determined via radioimmunoassay. RESULTS: The homocysteine levels of the patient group were significantly higher than those of the normal control group. The homocysteine level was more negatively correlated with the folate level in the schizophrenia group than in the control group. The percentages of female and male schizophrenia subjects manifesting high homocysteine levels were 33.8 and 51.5%, respectively. The percentage of schizophrenia subjects with low folate levels was 66.2%. In the low- and normal-folate-level groups, the patient group showed significantly higher homocysteine levels than the normal control group. The low-folate-level patient group particularly showed significantly higher homocysteine levels than the low-folate-level normal control group. CONCLUSION: Some schizophrenia patients with high serum homocysteine levels may have the genetic defect of having low folate serum levels. In such cases, folate ingestion may be a good management modality for clinical improvement.
Eating
;
Female
;
Fluorescence Polarization Immunoassay
;
Folic Acid
;
Homocysteine
;
Humans
;
Male
;
Schizophrenia
;
Virtues
;
Vitamin B 12
7.Therapeutic Drug Monitoring of Vancomycin.
Seong Heon WIE ; Sang Il KIM ; Yang Ree KIM ; Sung Mi BAE ; Kyoung Ja HONG ; Hyun Oh RA ; Moon Won KANG
Korean Journal of Infectious Diseases 2000;32(2):141-147
BACKGROUND: Vancomycin assay was performed to evaluate the clinical significance of therapeutic drug monitoring (TDM) of vancomycin and to compare pharmacokinetic parameters of vancomycin in our patients with population pharmacokinetic parameters. METHODS: In seventy-eight patients (45 males, 33 females), vancomycin serum concentrations were measured by fluorescence polarization immunoassay. At steady-state, peak levels were obtained one hour postinfusion, and trough levels were obtained just before a next dose. And also predicted serum vancomycin levels were determined by CAPCIL program in all subjects and compared with measured values, respectively. All patients were divided into two groups. Sixty-two patients in group I had a creatinine concentration in serum of < or =1.2 mg/dL and sixteen patients in group IIhad abnormal renal function as defined by a creatinine concentration in serum of >1.2 mg/dL. Follow-up second vancomycin concentrations were measured in 22 of 78 patients several days after initial TDM and were compared with initial TDM results. RESULTS: Mean values of peak and trough vancomycin concentration were 30.1 and 10.4, and 37.3 and 14.5 microgram/mL in groups I, and II, respectively. Predicted mean values of those were 26.3 and 9.2, and 28.2 and 7.8 microgram/mL in groups I, and II, respectively. Statistically significant differences between predicted and measured values in peak levels of group I,and IIand in trough level of group IIwere observed (P<0.01). Dose modification were required in 13 (21%) of 62 patients with normal renal function, and in 9 (56%) of 16 patients with abnormal renal function. Among 79 paired samples with a trough value below 15 mg/L, there were no peaks greater than 40 mg/L except two samples. CONCLUSION: Significant differences were noted between predicted and measured serum vancomycin concentrations and so TDM of vancomycin is needed to obtain effective dose and interval of vancomycin. More data about measured peak and trough values should be collected to establish pharmacokinetic parameters of vancomycin in Korean population.
Creatinine
;
Drug Monitoring*
;
Fluorescence Polarization Immunoassay
;
Follow-Up Studies
;
Humans
;
Male
;
Vancomycin*
9.Pharmacokinetics of Digoxin in Stomach Cancer Patients Undergoing a Gastrectomy under Enflurane Anesthesia.
Soo il LEE ; Hyung Ho KIM ; Byung Jin KIM ; Do Kyung LEE ; Chang Youl BAIK ; Jong Hwan LEE
Korean Journal of Anesthesiology 2002;43(1):26-32
BACKGROUND: Sometimes digoxin may be needed to be administered during anesthesia for the control of the ventricular rate and mitigation of lung congestion. For the lack of studies in surgical patients we wanted to determine digoxin pharmacokinetics (PK) in subjects undergoing a gastrectomy under enflurane anesthesia. METHODS: Over 72 hours, 16 serum samples from each of 14 subjects (ASA status 1 or 2) diagnosed with gastric cancer were collected after a single intravenous dose of digoxin 10ng/kg actual body weight for serum concentration measurements by fluorescence polarization immunoassay. Digoxin was injected 30 minutes after beginning surgery. Two, three and non-compartmental (TwC, ThC and NC, respectively) PK parameters were determined using WinNonln. The important parameters (AUC, Cl, Vss, t beta 1/2 [elimination half-life], Vc, MRT) of 3 PK's were statistically evaluated about which compartment PK was appropriate to the data here. The parameters of TwC PK of the surgical subjects were compared to those of nonsurgical ones which had already been published by other investigators. The postoperative outcomes (ambulation date, diet date, discharge date, complications, APACHE II and MOF [multiple organ failure]) in 19 patients (nondigoxin group) not treated with digoxin were compared with 19 patients (digoxin group) treated with digoxin 5ng/kg two times at 2 hour intervals during surgery. RESULTS: No significant differences were found in parameters between TwC and NC. Significant differences were observed in parameters of PK between ThC versus Twc and NC (P < 0.01). Cl and Vss of surgical subjects were larger than those of nonsurgical ones, and t beta 1/2 was similar. No significant effect of digoxin on postoperative outcomes was demonstrated. CONCLUSIONS: The dosage of digoxin for surgical patients should be titrated to the bleeding amount and the severity of surgical trauma because of larger Cl and Vss. The effect of digoxin on postoperative outcomes remains to be studied.
Anesthesia*
;
APACHE
;
Body Weight
;
Diet
;
Digoxin*
;
Dronabinol
;
Enflurane*
;
Estrogens, Conjugated (USP)
;
Fluorescence Polarization Immunoassay
;
Gastrectomy*
;
Hemorrhage
;
Humans
;
Lung
;
Pharmacokinetics*
;
Research Personnel
;
Stomach Neoplasms*
;
Stomach*
10.The Comparative Analysis of the Carotid Intima-Media Thickness and Homocysteine Level between Ischemic Stroke and Hypertensive Intracerebral Hemorrhage.
Dong Chul HAN ; Dong Jin SHIN ; Hyeon Mi PARK ; Cheol Wan PARK ; Yeong Bae LEE
Korean Journal of Cerebrovascular Surgery 2006;8(3):190-194
BACKGROUND: Carotid artery intima-media thickness (IMT) is an early structural marker of the atherosclerotic process and an elevated total homocysteine level is an early biochemical marker of atherosclerosis. But there are few reports about serum homocysteine level and carotid IMT between ischemic stroke, hypertensive intracerebral hemorrhage (HICH) and control group. METHOD: We studied about 173 patients with ischemic stroke, HICH and control group. Carotid IMT was defined as the mean of IMT measured by B-mode ultrasonography. Serum homocysteine level was measured by fluorescence polarization immunoassay method in fasting state. We compared serum homocysteine level and carotid IMT between ischemic stroke, HICH and control group. In statistics, One-Way ANOVA was used. RESULTS: A significant increase in carotid IMT was noted in ischemic stroke and HICH compared with that in the control group (p<0.05), whereas there was no significant differences in carotid IMT between ischemic stroke and HICH. The serum homocysteine level of ischemic stroke was significantly higher than that of control group (p<0.05). But there were no significant differences between HICH and control group, HICH and ischemic stroke. CONCLUSIONS: In our study, we thought a carotid IMT of ischemic stroke, HICH and serum homocysteine level in ischemic stroke can be used as early diagnostic marker. Therefore, our results address the need of further prospective clinical studies in patients with ischemic stroke and HICH in order to evaluate a possible diagnostic ability of carotid IMT and serum homocysteine level.
Atherosclerosis
;
Biomarkers
;
Carotid Arteries
;
Carotid Intima-Media Thickness*
;
Cerebral Hemorrhage
;
Fasting
;
Fluorescence Polarization Immunoassay
;
Homocysteine*
;
Humans
;
Intracranial Hemorrhage, Hypertensive*
;
Stroke*
;
Ultrasonography