Flow cytometer, a machine that utilizes the usage of light signals, has become one of the important machines or components in the studies of specific cell populations, cell surfaces, or even in detecting intracellular proteins. Due to its multiplex functionalities, while at the same time being very sensitive and specific, flow cytometer, or the technique of flow cytometry, has become a favourable and valuable tool for immunologists and clinicians for initial diagnosis of primary immunodeficiency diseases (PID), especially in studying cellular functional characteristics linked to PID. This paper will review key immunology tests of clinical flow cytometry, specifically in immunophenotyping, T cells classification and lymphocyte proliferation assays for their advantages and limitations in the field of PID. Importance of validation for the assays will be discussed. It is expected that development of validated immunology tests in clinical flow cytometry for PID diagnosis ensure standardized protocols, improve troubleshooting and disease interpretation, and improve the quality in patients’ diagnosis and management.
Flow cytometry

No abstract available.
Adenocarcinoma* ; Flow Cytometry*

No abstract available.
DNA* ; Flow Cytometry*

No abstract available.
DNA* ; Flow Cytometry*

No abstract available.
Flow Cytometry* ; Microscopy*

No abstract available.
Flow Cytometry* ; Microscopy*

No abstract available.
Flow Cytometry* ; Microscopy* ; Urinalysis*

In order to meet the needs of the flow cytometry for the simultaneous analysis of multiple fluorescence wavelengths and small volume, the design method of flow cytometry spectrum analysis system is presented by analyzing the characteristics of Dyson structure. And according to the method, a flow cytometry spectrum analysis system is disigned with Dyson type.The system's spectral range is 400 nm to 800 nm, the defocused spot size is less than the pixel size 24μ mm, the ransfer function value is above 0.8 at the Nyquist cut-off frequency 21 lp/mm,the spectral resolution is less than 3 nm, and the overall size is 83.54 mm×85.60 mm.The system has good optical performance and small volume, which meets the needs of the flow cytometry fluorescence spectral analysis. The outstanding innovation of this system is the application of Dyson light splitting structure and EMCCD detector which is high speed and high sensitivity.
Equipment Design ; Flow Cytometry ; instrumentation

No abstract available.
Flow Cytometry* ; Mastocytosis, Systemic* ; Splenomegaly*

Multiple myeloma is a common hematological malignancy which is characterized by plasma cell malignancies proliferation and osteolysis destruction. It manifests M-protein, bone destruction, anemia, renal function impairment and immune function abnormality. In recent years, researchers have known that flow cytometric immunophenotyping is valuable in diagnosis, therapy, prognosis and minimal residual disease detection of multiple myeloma. The review summarizes the development of flow cytometric immunophenotyping in multiple myeloma, including application of flow cytometry in multiple myeloma and immunophenotypic features of flow cytometric analysis in multiple myeloma.
Flow Cytometry ; Humans ; Immunophenotyping ; Multiple Myeloma ; immunology