1.Expression of angiopoietin-1 and 2 in squamous cell carcinoma of the head and neck areas and normal mucosa.
Mei-Hua ZHENG ; Florian VEELKEN ; Daniel LÁBBE ; Wilhelm BLOCH ; Olaf MICHEL
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(5):371-375
OBJECTIVETo study the significance by analyzing the expression of angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) in the tissues of squamous cell carcinoma (SCC) of the head and neck areas and in normal mucosa tissues.
METHODSThe expression of Ang-1 and Ang-2 in 45 tumor samples and 7 normal mucosa tissues were determined by the immunohistochemical method with avidin-biotin-peroxidase complex technique. The results were scored by two independent observers and analyzed statistically.
RESULTSThe positive expression of Ang-1 and Ang-2 existed in the endothelial cells, epithelial cells and also in SCC cells. The positive expression rates of Ang-1 in tumor samples was 78% in the endothelial cells, and 87% in SCC cells. The positive expression rates of Ang-2 in tumor samples was 69% in the endothelial cells, and 76% in SCC cells. The scores of positive expression of Ang-1 and Ang-2 were higher in endothelial cells and in SCC cells of tumor tissues than that of normal mucosa tissues (rank sum test, P < 0. 05). There was positive correlation between the expression of Ang-1 and Ang-2 in the endothelial cells and also in SCC cells (Chi-square test with contingency table, P < 0.05). Ang-1 expression in endothelial cells of tumor tissues was higher in clinical stage III-IV than that in clinical stage I-II (rank sum test, P < 0.05). Ang-2 expression in both endothelial cells and SCC cells, were higher in clinical stage II-IV than that in clinical stage I-II (rank sum test, P < 0.05). There was no statistical significance for degrees of Ang-1 and Ang-2 expression in different histological grades (P > 0.05).
CONCLUSIONSThe expressions of Ang-1 and Ang-2 in advanced SCC were remarkable. Ang-1 and Ang-2 may play a critical role during the progress of SCC of head and neck areas.
Adult ; Aged ; Angiopoietin-1 ; metabolism ; Angiopoietin-2 ; metabolism ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Female ; Head and Neck Neoplasms ; metabolism ; pathology ; Humans ; Male ; Middle Aged ; Mouth Mucosa ; metabolism ; Neoplasm Staging ; Vascular Endothelial Growth Factor A ; metabolism
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