From the leaves of Eurya ciliata(Merr),Chrysoeriol, a flavonoide, was isolated.The 13C-NMR reassignment of Chrysoeriol is based on the analysis of 2D-NMR experiments
Flavonoids ; Plant Leaves ; isolation & purification

OBJECTIVETo study the chemical constituents of Phellinus igniarius.

METHODCompounds were isolated by nomal phasc sillica gel and Al2O3 chromatography and reverse phase HPLC. Their structures were elucidated by spectroscopic methods including IR, MS and NMR.

RESULTFive flavonoids and two coumarins were isolated from Phellinus igniarius and their structures were identified as naringenin, sakuranetin, aromadendrin, folerogenin, eriodictyol, coumarin and scopoletin.

CONCLUSIONAll these compounds were obtained from this genus for the first time.

Flavanones ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification ; Polyporaceae ; chemistry ; Scopoletin ; chemistry ; isolation & purification

Nine flavonoids were isolated and identified as luteolin (1), luteolin-4'-O-beta-D-glucoside (2), acacetin-7-O-beta-D-glucoside (3), rutin (4), acacetin (5), quercetin (6), quercetin-3-O-beta-D-glucoside (7), kaempferol-3-O-beta-D-glucoside (8), Isorhamnetin-3-O-beta-D-glucoside(9) from Lysimachia paridiformis var. stenophylla, and all these compounds were isolated from this plant for the first time.
Antioxidants ; analysis ; isolation & purification ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Flavonoids ; analysis ; isolation & purification ; Primulaceae ; chemistry

OBJECTIVETo study the chemical constituents of the whole plants of Pedicularis densispica.

METHODThe chemical constituents were isolated by various chromatographic methods and their structures were determined by chemical evidences and spectral data.

RESULTTen compounds were isolated and identified as acacetin (1), apigenin-7-0-beta-glucopyranoside (2), kaempferol-3,7-O-alpha-dirhamnopyranoside (3), scutellarein-7-0-beta-glucopyranoside (4), chrysoeriol-7-O-beta-glucopyranoside (5), pedicutricone A (6), dearabinosyl pneumonanthoside (7), salidroside (8), darendoside B (9), and maltol-beta-D-glucopyranoside (10).

CONCLUSIONThese compounds were isolated from the titled plant for the first time. Except compounds 6 and 8, the others were obtained for the first time from the genus Pedicularis.

Drugs, Chinese Herbal ; analysis ; isolation & purification ; Flavonoids ; analysis ; isolation & purification ; Pedicularis ; chemistry ; Sesquiterpenes ; analysis ; isolation & purification

Phytochemical investigation of the flowers of Hosta plantaginea led to isolate of one new flavonoid glycoside,plantanone C( 1) by silica gel,Sephadex LH-20,and RP-HPLC column chromatographies. Its structure was extensively determined on basis of HR-ESI-MS and NMR spectroscopic data. Compound 1 exhibited moderate antioxidant activity against DPPH radical scavenging activity,with an IC50 value of 240. 2 μmol·L~(-1).
Antioxidants ; analysis ; isolation & purification ; Flavonoids ; analysis ; isolation & purification ; Flowers ; chemistry ; Glycosides ; analysis ; isolation & purification ; Hosta ; chemistry

OBJECTIVETo investigate the flavonoids in leaves of Alstonia scholaris, an ethno-remedy of Dai People in Yunnan.

METHODThe chemical constituents were isolated and purified by column chromatography. Their structures were elucidated on the basis of spectroscopic evidences and physicochemical properties.

RESULTEight flavonoids were isolated and identified as kaempferol (1), quercetin (2), isorhamnetin (3), kaempferol-3-0-beta-D-galactopyranoside (4), quercetin-3-0-beta-D-galactopyranoside (5), isorhamnetin-3-0-beta-D-galactopyranoside (6), kaempferol-3-0-beta-D-xylopyranosyl-( 2-1)-0-beta-D-galactopyranoside (7) ,quercetin-3-0-beta-D-xylopyranosyl-( 2-1)-0-beta-D-galactopyranoside (8).

CONCLUSIONFlavonoids 1-7 were isolated from A. scholaris for the first time.

Alstonia ; chemistry ; Flavonoids ; analysis ; isolation & purification ; Plant Leaves ; chemistry

OBJECTIVETo investigate the chemical constituents of the roots of Flemingia philippinensis.

METHODSilica gel, ODS silica gel and Sephadex LH-20 column chromatography were employed for the isolation and purification. The structures were identified on the basis of spectral data (MS, 1H-NMR and 13C-NMR) and chemical evidence.

RESULTSeven compounds were isolated from the 75% ethanolic extract of the roots of F. philippinensis and identified as follows: flemiphilippinin D (1), dorsmanins I (2), osajin (3), eriosematin (4), lupinalbin A (5), genistein (6) and 3'-O-methylorobol (7).

CONCLUSIONThe compounds 2, 3 and 4 were isolated from the genus Flemingia for the first time. The compounds 2 and 4 were obtained from the Flemingia philippinensis for the first time.

Fabaceae ; chemistry ; Flavonoids ; analysis ; isolation & purification ; Plant Roots ; chemistry

OBJECTIVETo study the chemical constituents of Memorialis hirta.

METHODCompounds were isolated and purified by multiple methods, and their structures were identified based on physicochemical property and spectrum data.

RESULT12 compounds were isolated from ethyl acetate from 95% ethanol extracts of M. hirta, they were isorhamnetin (1), kaempferol (2), quercetin (3), isorhamnetin-3-O-alpha-L-rhamnopyranoside (4), kaempferol-3-O-alpha-L-rhamnopyranoside (5), isorhamnetin-3-O-beta-D-glucopyranoside (6), kaempferol-3-O-beta-D-glucopyranoside (7), quercetin-3-O-alpha-L-rhamnopyranoside (8), quercetin-3-O-beta-D-glucopyranoside (9), isorhamnetin-3-O-rutinoside (10), kaempferol-3-O-rutinoside (11) and quercetin-3-O-rutinoside (12), respectively.

CONCLUSIONAll compounds were obtained from the genus Memorialis for the first time.

Drugs, Chinese Herbal ; chemistry ; Flavonoids ; analysis ; isolation & purification ; Urticaceae ; chemistry

This study is to explore the reason of "the older, the better" of PCR and itsincrease of flavonoids. We identified the fun- gus isolated from the PCR using microscopic and molecular identification. HPLC method was used to determine the content of 4 fla- vonoids and to clarifythe regularity of them; UV spectrophotometry method was used to determine the total content of flavonoids; reverse thinking was applied to screen the fungus that have close relation to the change of flavonoids. Finally, we have isolated and identified 25 fungusfrom the PCR, which belong to 2 genus and 4 species, including pencillium commune, P. minioluteeum, P. citrinum, Aspergillus flavus and A. niger. The content of flavonoids was increased in the mildew PCR due to A. niger and other fungus. Therefore, "the ol- der, the better" of PCR had its scientific reason that the increase of flavonoids had a close relation of the metabolic activity of A. niger and other fungus.
Citrus ; chemistry ; microbiology ; Flavonoids ; analysis ; Fungi ; isolation & purification

Derris eriocarpa, a traditional Chinese medicine belonging to the family of Leguminosae, is widely distributed mainly over Yunnan, Guangxi and Guizhou of China. Modern pharmacological researches on this herb showed that it had extensive bioactivities, such as promoting urination, removing dampness and cough and reducing inspissated mucus and other biological activities. The extensive studies on the chemical constituents of this plant have resulted in the isolation of triterpenoids, steroids, fatty acid and others, but the flavone compounds haven't reported before. In our further research on the ethyl acetate of this plant, nine flavone compounds were obtained by column chromatography on silica gel, Sephadex LH-20, semi-prep HPLC, polyamide column chromatography and recrystallization for separation and purification. The structures were determined on the basis of extensive spectroscopic analysis, including MS, NMR experiments and comparison with spectroscopic data in the literature, respectively, as diosmetin (1), 3, 3'-di-O-methylquercetin (2), afromosin (3), 6, 3'-dihydroxy-7, 4'-dimethoxyisoflavone (4), odoratin (5), 7, 3'-dihydroxy-8, 4'-dimethoxyisoflavone (6), 6, 4'-dihydroxy-7, 3'-dimethoxyisoflavone (7), 5, 7, 4'-trihydroxy-3, 3', 5'-trimethoxyflavone (8), and alpinumisoflavone (9). All these compounds were isolated from Derris eriocarpa How for the first time. And the in vitro assays showed that compound 2 possessed moderate inhibitory activity against human cancer cells K562 and HEL.
Derris ; chemistry ; Flavonoids ; chemistry ; isolation & purification ; pharmacology ; Humans ; K562 Cells