Five compounds were isolated from Capsicum annuum by means of various chromatographic techniques (silica gel, Sephadex LH-20, MCI GEL CHP-20P and HPLC), and their structures were determined as luteolin-7-O-[2"-O-(5"-O-sinapoyl)-beta-D-apiofuranosyl]-beta-D-glucopyranoside (1), uridine (2), adenosine (3), 7-hydroxy-6-methoxy cinnamic acid ethyl ester (4) and 7-hydroxy cinnamic acid ethyl ester (5) by extensive spectroscopic analyses (UV, IR, MS, 1D- and 2D-NMR). Among them, compound 1 is a new flavone glycoside named as capsicuoside A, and cmpounds 2-5 are isolated for the first time from the fruits of C. annuum.
Capsicum ; chemistry ; Flavones ; chemistry ; Fruit ; chemistry ; Glucosides ; chemistry

Using bioactive compounds 7-hydroxy flavone, salicylaldehyde, cinnamic acid and 4-amino-5- mercapto-1,2,4-triazoles as starting materials, three new types of flavone derivatives containing 1,2,4-triazole structure were synthesized via different step reactions. These new compounds were characterized by 1IHNMR, ESI-MS, IR and elemental analysis. Their scavenging effects on the superoxide radical (O2·-), hydroxyl radical (·OH), DPPH · radical and their total reduction activities were tested. The results showed that all of the compounds possessed some antioxidative activity at the concentration of 0.5 mg · mL(-1), but the scavenging ability of the target compounds was lower than that of the standard compound Vc.
Cinnamates ; chemistry ; Flavones ; chemical synthesis ; chemistry ; Flavonoids ; chemistry ; Free Radical Scavengers ; chemical synthesis ; chemistry ; Triazoles ; chemistry

OBJECTIVETo investigate the chemical constituents in flower buds of Artemisia scoparia.

METHODThe constituents were separated and purified by means of various chromatographic techniques, and their structures were determined on the basis of physical and chemical properties and spectral data.

RESULTFour flavones were isolated and their structures were identified as cirsilineol (I), cirsimaritin(II), arcapillin(III) and cirsiliol(IV) respectively.

CONCLUSIONCompounds I, III and IV were isolated from this plant for the first time.

Artemisia ; chemistry ; classification ; Flavones ; Flavonoids ; chemistry ; isolation & purification ; Flowers ; chemistry ; Plant Leaves ; chemistry ; Plants, Medicinal ; chemistry

OBJECTIVETo optimize the process of extracting effective constituents from lotus leaf.

METHODIndependent variables were ethanol concentration reflux time and solvent fold, dependent variables were extraction rates of nuciferine and flavone in lotus leaf, central composite design and response surface methodology were used for optimization of extraction of lotus leaf.

RESULTThe optimum conditions of extraction process were 75% -80% ethanol, 2-3 hours for reflux, 20-25 fold solvent and 2 times for extraction. Bias between observed and predicted of rates of nuciferine and flavone values were 5.53%, -6.02%, respectively.

CONCLUSIONThe values observed and predicted were close to each other, which proved that the optimization of of extraction of lotus leaf by central composite design and response surface methodology was reasonable and successful.

Aporphines ; chemistry ; isolation & purification ; Flavones ; Flavonoids ; chemistry ; isolation & purification ; Loteae ; chemistry ; Plant Leaves ; chemistry ; Reproducibility of Results

The chemical constituents in the Pu-erh tea were isolated by various column chromatographic methods, and their structures were identified by spectral data and physic-chemical analysis. As a result, eleven flavonoids were isolated and identified as kaempferol (1), quercetin (2), kaempferol-3-O-beta-D-glucopyranoside (3), kaempferol-3-O-beta-D-rutinoside (4), apigenin-6-C-alpha-L-arabinopyranosyl-8-C-beta-D-glucopyranoside (5), vitexin-4"-O-beta-D-glucopyranoside (6), kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1--> 3)-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside] (7), quercetin3-O-beta-D-glucopyranoside (8), rutin (9), quercetin-3-0-alpha-L-rhamnopyranoside (10), and myricetin-3-0-beta-D-glucopyranoside (11). Compounds 5-7, 10 and 11 were isolated from Pu-erh tea for the first time.
Camellia sinensis ; chemistry ; Flavones ; chemistry ; Mass Spectrometry ; Molecular Structure ; Plant Extracts ; chemistry ; Tea ; chemistry

AIMTo study the chemical constituents of the stem of Fordia cauliflora of Yunnan province.

METHODSThe constituents were separated and purified by repeated silica column chromatography. The structures were elucidated by physical-chemical properties and spectroscopic data.

RESULTSSix compounds were isolated from the ethanol extract of the stem of Fordia cauliflora. They were identified as: 6-hydroxy-3-methoxy-6",6"-dimethylchromeno-(2", 3" : 7, 8)-flavone (1), 3-methoxy-6-(3-methyl-but-2-enyloxy)-6", 6"-dimethylchromeno-( 2", 3" : 7, 8)-flavone (2), 3, 6-dimethoxy-6", 6"-dimethylchromeno-( 2", 3" : 7, 8)-flavone (3), 7-hydroxy-4'-methoxyisoflavone (4), 7, 4'-dihydroxyisoflavone (5) and karanjin (6).

CONCLUSIONCompounds 1 and 2 are new compounds. Compounds 3 -5 were isolated from the plant for the first time.

Fabaceae ; chemistry ; Flavones ; chemistry ; isolation & purification ; Molecular Structure ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry

The recent progresses on chemical components and pharmacological activities of the genus Gentiana were summarized. The main chemical constituents of this genus are iridoids, xanthone and flavone. There were more than ninety iridoid compounds, one hundred xanthone compounds, and thirty four flavone compounds isolated up till now. And flavone compounds were obtained mainly from aerial part. The plants of this genus had broad bioactivities such as antifungal activity, anti-inflammatory activity and liver protective effect. These mentioned above would be helpful for further studies on the plants of the genus Gentiana.
Animals ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Flavones ; Flavonoids ; chemistry ; pharmacology ; Gentiana ; chemistry ; Humans ; Xanthones ; chemistry ; pharmacology

OBJECTIVETo study the chemical constituents in the spikes of Schizonepeta tenuifolia.

METHODCompounds were isolated and purified with silica gel, ODS and Sephadex LH-20 gel column chromatography, and their structures were determined by using spectroscopic analysis including MS and NMR.

RESULTNine compounds were isolated and identified as 5, 7-dihydroxy-6, 4'-dimethoxyflavone (1), 5, 7-dihydroxy-6, 3', 4'-trimethoxyflavone (2), ursolic acid (3), 3-hydroxy-4(8)-ene-p-menthane-3(9)-lactone (4), 5, 7, 4'-trihydroxyflavone (5), 5, 4'-dihydroxy-7-methoxyflavone (6), hesperidin (7), luteolin (8) and daucesterol (9).

CONCLUSIONCompounds 1, 2, 6 were first obtained from the spikes of S. tenuifolia.

Flavones ; chemistry ; isolation & purification ; Flowering Tops ; chemistry ; Lamiaceae ; chemistry ; Plants, Medicinal ; chemistry

OBJECTIVETo investigate the chemical constituents of whole herbs of Seriphidium terrae-albae.

METHODVarious chromatographic techniques were employed for the isolation and purification of the chemical constituents. The structures were elucidated by chemical and spectral analysis.

RESULTSeven flavonoid compounds were isolated and identified as apigenin, eupatilin, chrysoeriol, quercetin-3, 3'-dimethyl ether, quercetagetin-3, 6-dimethyl ether, quercetagetin-3, 6-dimethyl ether-7-O-beta-D-pyranoglucoside and quercetagetin-3, 6-dimethyl ether-4'-O-beta-D-pyranoglucoside, respectively.

CONCLUSIONAll of these flavonoid compounds were isolated from the plant for the first time.

Apigenin ; chemistry ; isolation & purification ; Asteraceae ; chemistry ; Flavones ; Flavonoids ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry

OBJECTIVETo evaluate the performance of AB-8 macroporous adsorption resin for adsorption and desorption of flavones in liquorice.

METHODSThe concentration of flavones in liquorice was determined by ultraviolet spectrophotometry, and the adsorption behavior of AB-8 macroporous adsorption resin to flavones in liquorice was examined for the adsorption capacity and the volume of solution loaded.

RESULTSOptimal adsorption of flavones was achieved with the sample pH of 5, total flavones concentration in the solution of 0.85 mg/ml, sample flow velocity of 3 BV/h, and washing with 60% ethanol at the flow velocity of 3 BV/h.

CONCLUSIONAB-8 macroporous adsorption resin can be well applicable for enrichment of flavones in liquorice.

Adsorption ; Flavones ; chemistry ; isolation & purification ; Glycyrrhiza ; chemistry ; Macromolecular Substances ; chemistry ; Porosity ; Resins, Synthetic ; chemistry ; Spectrophotometry, Ultraviolet