1.Evaluation of modified vaccinia virus Ankara expressing VP2 protein of infectious bursal disease virus as an immunogen in chickens.
Flavia Adriana ZANETTI ; Maria Paula Del Medico ZAJAC ; Oscar Alberto TABOGA ; Gabriela CALAMANTE
Journal of Veterinary Science 2012;13(2):199-201
A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Besides, as the epitopes of VP2 responsible to induce IBDV-neutralizing antibodies are discontinuous, our results suggest that VP2 protein expressed from MVA-VP2 maintained the correct conformational structure. To our knowledge, this is the first report on the usefulness of MVA-based vectors for developing recombinant vaccines for poultry.
Animals
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Antibodies, Viral
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Birnaviridae Infections/prevention & control/*veterinary
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Cells, Cultured
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Chick Embryo
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Chickens
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Fibroblasts/metabolism
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Infectious bursal disease virus/*immunology
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Poultry Diseases/*prevention & control/virology
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Specific Pathogen-Free Organisms
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Vaccinia virus/*genetics/immunology/metabolism
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Viral Structural Proteins/genetics/*immunology/metabolism
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Viral Vaccines/*immunology