Aims:To evaluate the effectivity of Lactobacillus plantarumBSL isolated from Indonesian sauerkraut against Listeriamonocytogenes ATCC 7644through in vitroand in vivoassay. Methodology and results:In vitroexamination for antimicrobialactivity against L.monocytogenesATCC 7644was performed using seven isolates of lactic acid bacteria (LAB). LactobacillusplantarumBSL demonstrated the highest activity against L. monocytogenesandstudied further in Sprague-Dawley (SD) rats. Treatmentgroup of rats received 0.5 mL culture suspension (109CFU/mL) of L. plantarumBSL and control group received 0.5 mL of 0.85% w/v NaCl daily during nine days of treatment. Both groups were infected at 3rd day with0.5 mL of suspension of L. monocytogenes (109CFU/mL). At the 2nd(before infection), 5th, 7th, and 9thday (after infection), the rats were sacrificed and the faeces, caecum, and caecum content were examined for the population of LAB and L. monocytogenes. Administration of L. plantarumBSL significantly increased the population of LABby 1.2–1.4 log unit, while the number of L. monocytogeneswas reduced by 1.8–1.9 log unitcompared to control group eithr in the faeces, caecum, or caecum content. Administration of L. plantarumBSLcould be able to reduce the liver and spleen damageof the experimental rats, butdid not show any changes in immunoglobulin A (IgA) response in comparison with control group. Conclusion, significance and impactofstudy: LactobacillusplantarumBSL was promising as probiotic candidate with health promotion to protect the gastrointestinal from infection by L. monocytogenesATCC 7644.