1.Double bridge PAP labelling of fibronectin in paraffin processed tissue.
Douk Ho HWANG ; Young Seok KIM ; In Yup CHANG ; Wang Jae LEE ; Ka Young CHANG
Korean Journal of Anatomy 1991;24(2):260-167
No abstract available.
Fibronectins*
;
Paraffin*
2.Immunohistochemical localization of fibronectin during experimental enchondral bone formation.
Young Ho KWON ; Jae Do KIM ; Jae Hee SEO ; Man Ha HUH
The Journal of the Korean Orthopaedic Association 1992;27(4):1172-1177
No abstract available.
Fibronectins*
;
Osteogenesis*
3.Comparison of plasma fibronectin in preeclampsia of before delivery and post delivery.
Chan LEE ; Jun MOON ; Eun Hee LEE ; Dong O KIM ; Chan Il PARK ; Jun Yong HUR ; Ho Suk SUH ; Yong Gyun PARK ; Kap Soon JU ; Soo Yong CHOUGH
Korean Journal of Perinatology 1993;4(3):305-314
No abstract available.
Fibronectins*
;
Plasma*
;
Pre-Eclampsia*
4.Binding of fibronectin to staphylococcus aureus.
Jung Wan KIM ; Sang Hwa LEE ; Yoo Chul LEE ; Sung Yong SEOL ; Dong Taek CHO
Journal of the Korean Society for Microbiology 1993;28(6):431-441
No abstract available.
Fibronectins*
;
Staphylococcus aureus*
;
Staphylococcus*
5.In vivo assessment of Fibroblast growth factor(FGF)- Fibronectin fusion protein coating on titanium: Histomorphometric analysis in rabbit tibia.
Ho Kyun NA ; Tae Il KIM ; Sang Hoon LIM ; Ki Young CHO ; Chong Pyoung CHUNG ; Soo Boo HAN ; Young KU
The Journal of the Korean Academy of Periodontology 2005;35(1):153-161
No abstract available.
Fibroblasts*
;
Fibronectins*
;
Tibia*
;
Titanium*
7.The effect of homologous exogenous fibronectin in wound healing.
Kyung Tae YOUN ; Jin Suk BYUN ; Bong Soo BAIK ; Woon E BAIK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(6):916-929
No abstract available.
Fibronectins*
;
Wound Healing*
;
Wounds and Injuries*
9.Mesenchymal Stem Cells: The Promotion of Endodermal-Induction Using Activin A.
Sang Woo LEE ; Seon Ok MIN ; Shin Young KIM ; Sae Byeol CHOI ; Hyun Ok KIM ; Kyung Sik KIM
Korean Journal of Hepato-Biliary-Pancreatic Surgery 2009;13(4):205-214
PURPOSE: The most important consideration for therapy using MSCs is the differentiation of the target organ's cell type. For in-vitro hepatogenic differentiation of MSCs, the main focus is efficient induction of the MSCs into the endoderm stage. Activin A, which is a signaling molecule that is similar to Nodal, promotes the induction of definitive endoderm from both ESs and MSCs. The protocols for induction into definitive endoderm have shown different efficiency and reproducibility depending on the researchers or the sources of the MSCs. Thus, a study on the various conditions of Activin A is needed to efficiently differentiate MSCs into the definitive endoderm lineage of MSCs. METHODS: MSCs were isolated from human adipose tissues and these were cultured in MCM (MSCs Culture Medium) on a human fibronectin coated plate. At 70~80% confluence, the MSCs were harvested and cultured in MCM supplemented with Activin A, at a 50 ng/mL concentration, and FGF4. The expression of the genes related with MSCs or primitive endoderm were analyzed by RT-PCR. The changes of cell morphology for differentiation were also observed by a light microscope & a SEM. RESULTS: The expression of genes related with primitive foregut endoderm was seen in the groups that were treated with a higher concentration of Activin A. The morphology of the cells that differentiated into definitive endoderm were not different from those of the undifferentiated MSCs. The expression of genes related with functional primitive hepatocytes was seen in the early phase during hepatic differentiation. The cell morphology was changed to a similar cuboidal form in a time-dependent manner. CONCLUSION: Activin A promotes a more rapid induction of definitive endoderm. It also makes an efficient condition for the differentiation into primitive foregut endoderm at a higher concentration.
Activins
;
Endoderm
;
Fibronectins
;
Hepatocytes
;
Humans
;
Light
10.Mesenchymal Stem Cells: The Promotion of Endodermal-Induction Using Activin A.
Sang Woo LEE ; Seon Ok MIN ; Shin Young KIM ; Sae Byeol CHOI ; Hyun Ok KIM ; Kyung Sik KIM
Korean Journal of Hepato-Biliary-Pancreatic Surgery 2009;13(4):205-214
PURPOSE: The most important consideration for therapy using MSCs is the differentiation of the target organ's cell type. For in-vitro hepatogenic differentiation of MSCs, the main focus is efficient induction of the MSCs into the endoderm stage. Activin A, which is a signaling molecule that is similar to Nodal, promotes the induction of definitive endoderm from both ESs and MSCs. The protocols for induction into definitive endoderm have shown different efficiency and reproducibility depending on the researchers or the sources of the MSCs. Thus, a study on the various conditions of Activin A is needed to efficiently differentiate MSCs into the definitive endoderm lineage of MSCs. METHODS: MSCs were isolated from human adipose tissues and these were cultured in MCM (MSCs Culture Medium) on a human fibronectin coated plate. At 70~80% confluence, the MSCs were harvested and cultured in MCM supplemented with Activin A, at a 50 ng/mL concentration, and FGF4. The expression of the genes related with MSCs or primitive endoderm were analyzed by RT-PCR. The changes of cell morphology for differentiation were also observed by a light microscope & a SEM. RESULTS: The expression of genes related with primitive foregut endoderm was seen in the groups that were treated with a higher concentration of Activin A. The morphology of the cells that differentiated into definitive endoderm were not different from those of the undifferentiated MSCs. The expression of genes related with functional primitive hepatocytes was seen in the early phase during hepatic differentiation. The cell morphology was changed to a similar cuboidal form in a time-dependent manner. CONCLUSION: Activin A promotes a more rapid induction of definitive endoderm. It also makes an efficient condition for the differentiation into primitive foregut endoderm at a higher concentration.
Activins
;
Endoderm
;
Fibronectins
;
Hepatocytes
;
Humans
;
Light
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