1.Result of serology test for yellow fever virus (YFV) in the entry-exit persons in Guangzhou frontier port and the forecast of risk on YFV infection.
Man-quan LI ; Jin-feng ZHANG ; Jian-ming ZHANG ; Wei KE ; Jing BAI ; Yun-kai YANG ; Rong-rong LIANG ; Ying MENG ; Yan-rong ZHOU ; Mei-li CHEN
Chinese Journal of Epidemiology 2012;33(5):544-545
2.Serologic studies of Xinjiang hemorrhagic fever in Bachu county, 2001.
Lei HAN ; Qing TANG ; Xiuqin ZHAO ; Masayuki SAIJO ; Xiaoxia TAO
Chinese Journal of Epidemiology 2002;23(3):179-181
OBJECTIVETo investigate the situation of Xinjiang hemorrhagic fever (XHF) in patients who have been diagnosed as XHF by clinical methods and to predict the condition in people who were liable to infection and in the host-animals.
METHODSSera collected from XHF patients and some peasants under the risk of contracting the disease, followed by checking the specific antibody against XHF with IgG-ELISA and IgM capture ELISA, and XHF viral antigen with antigen capture ELISA. In addition, 80 sheep/goats serums were collected from two places where there were more XHF cases and specific IgG antibody against XHF checked by ELISA method.
RESULTSPositive rate of IgG and IgM antibodies were 39.62% (21/53) and 20.75% (11/53) respectively in the serums of patients; one patient's serum showed XHFV antigen positive by antigen capture ELISA. IgG antibody positive rate for peasants' sera was 21.05% (4/19), but IgM antibody detection showed negative for all sera. In sera from 80 sheep and goats, 70% (56/80) showed IgG positive.
CONCLUSIONResults showed that XHF broke out in Bachu county from April to June 2001 while recessive infection of the disease remained serious.
Animals ; Antibodies, Viral ; blood ; Antigens, Viral ; blood ; China ; epidemiology ; Goats ; Hemorrhagic Fever Virus, Crimean-Congo ; immunology ; Hemorrhagic Fever, Crimean ; blood ; epidemiology ; virology ; Humans ; Immunoglobulin G ; blood ; Immunoglobulin M ; blood ; Mice ; Mice, Inbred BALB C ; Sheep
3.Investigation of Tahyna virus infection among unknown fever cases in Xinjiang, China.
Zhi LV ; Shi-hong FU ; Feng-tian WANG ; Olga L KOSOY ; Roger S NASCI ; Guo-dong LIANG
Chinese Journal of Virology 2011;27(1):71-74
To investigate the infection status and the spatial distribution of Tahyna virus infection among unknown fever cases in Xinjiang, China. Sera samples of unknown fever cases from Kashi in southern Xin-jiang and Yili in northern Xinjiang were tested against Tahyna virus by IFA. Partial positive cases were tested against Tahyna virus/Snowshoe hare virus/Inkoo virus parrelled. Finally, 742 sera samples of unknown fever cases were collected from Kashi, Southern Xinjiang in 2007-2008, the positive rate of IgM antibody against Tahyna virus was 5.3%, the positive rate of IgG antibody against Tahyna virus was 18.3%. 222 sera samples of unknown fever cases were collected from Yili, Northern Xinjiang in 2008, no positive case of IgM antibody against Tahyna was found. 10 cases showed antibody neutralization against Tahyna virus by plaque reduction neutralization test. Our results demonstrate that there is current infection and past infection of Tahyna virus among Southern Xinjiang residents.
Adolescent
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Adult
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Antibodies, Viral
;
blood
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Child
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Child, Preschool
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China
;
epidemiology
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Encephalitis Virus, California
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immunology
;
physiology
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Encephalitis, California
;
blood
;
epidemiology
;
virology
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Female
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Fever
;
blood
;
epidemiology
;
virology
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Humans
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Immunoglobulin M
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blood
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Infant
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Male
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Middle Aged
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Young Adult
4.Sero-survey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea.
Seong In LIM ; Chang Hee KWEON ; Dong Seob TARK ; Seong Hee KIM ; Dong Kun YANG
Journal of Veterinary Science 2007;8(1):45-49
Vector-borne arboviruses produce mild to severe symptoms in domestic animals. Bovine ephemeral fever (BEF), Akabane, Aino, and Chuzan virus have been primarily attributed to reproductive disorders or febrile diseases in cattle, and Japanese encephalitis virus (JEV) is mainly associated with reproductive failures in swine. We investigated antibody titers from domestic swine against four bovine arboviruses (BEF, Akabane, Aino, and Chuzan virus) and from cattle against JEV in Korea. While the positive rates for Akabane and BEF were 37.4% and 15.7%, the positive incidence of Chuzan and Aino were relatively low, with positive rates of 3.04% and 0.4%, respectively, based on a virus neutralization assay. Antibody titers against more than one virus were also frequently detected in domestic swine. The incidence of JEV was 51.3% among domestic cattle. In addition, one positive case was detected in the thoracic fluids from 35 aborted calves, based on the hemagglutination inhibition test. Our results indicate that swine are susceptible hosts of bovine arboviruses without showing clinical symptoms in a natural environment. Moreover, we confirmed that JEV could be associated with reproductive failure in pregnant cattle, as were other vector-borne bovine arboviruses assessed in this study.
Animals
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Antibodies, Viral/blood
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Cattle
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Cattle Diseases/*epidemiology/*virology
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Encephalitis Virus, Japanese/*immunology
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Encephalitis, Japanese/blood/epidemiology/*veterinary/virology
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Ephemeral Fever/blood/*epidemiology/virology
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Ephemeral Fever Virus, Bovine/*immunology
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Hemagglutination Tests
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Incidence
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Korea/epidemiology
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Neutralization Tests
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Swine
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Swine Diseases/*epidemiology/*virology
5.Surveillance on hemorrhagic fever with renal syndrome in Jiangsu province from 1996 to 2000.
Rong-qiang ZU ; Yang-sheng WU ; Feng-cai ZHU ; Guang-zhong LIU
Chinese Journal of Epidemiology 2003;24(2):109-112
OBJECTIVETo analyze the incidence rate, animal hosts, and human inapparent infection rate from surveillance data collected in Jiangsu province from 1996 to 2000 and the risk factors of hemorrhagic fever with renal syndrome (HFRS).
METHODSData on the incidence rate was collected from the whole province to describe the epidemiologic characteristics of HFRS. Data on the density of rats, proportion of rats with virus, index of rats with virus and the human inapparent infection rate were collected in special areas according to the standardised protocol in the project.
RESULTSThe incidence rate of HFRS reduced continuously in Jiangsu province, with a 42.17% reduction from 1996 to 2000. The ratio between the incidence rate of autumn-winter peak and spring peak had also reduced. The main host in spring was Rattus norvegicus (with a density of 3.07%), while that in autumn was Apodemus agrarius (with a density of 4.64%). The density of main hosts and mixed species of rats had all reduced during the five years of observation. The proportions of Rattus norvegicus, Apodemus agrarius, Mus musculus with virus were relatively high in spring and autumn. The index of Rattus norvegicus and Mus musculus with virus in spring were 0.025 5 and 0.028 4 respectively, while that of Apodemus agrarius with virus in autumn was 0.030 2. The average human inapparent infection rate was 4.73% in the five years.
CONCLUSIONThe incidence rate of HFRS in Jiangsu reduced significantly from 1996 to 2000, but the risk factors still widely existed. Suggesting that the surveillance program needs to be carried out steadily in Jiangsu.
Adult ; Animals ; Antibodies, Viral ; blood ; China ; epidemiology ; Disease Reservoirs ; Hemorrhagic Fever with Renal Syndrome ; epidemiology ; transmission ; Humans ; Incidence ; Middle Aged ; Muridae ; virology ; Rats ; virology ; Risk Factors ; Seasons ; Time Factors
6.Production of Chemokines in Kawasaki Disease, Henoch-Schonlein Purpura and Acute Febrile Illness.
Hyo Seok CHUNG ; Hyo Young KIM ; Hee Sun KIM ; Hee Jung LEE ; Ji Hyun YUH ; Eun Sil LEE ; Kwang Hae CHOI ; Young Hwan LEE
Journal of Korean Medical Science 2004;19(6):800-804
We compared the production of three chemokines; interferon-gamma-inducible protein-10 (IP-10), monocyte chemoattractant protein-1 (MCP-1) and growth-related onco-gene-alpha(Gro-alpha) that attracts monocytes or neutrophils, or both, in peripheral blood at acute stage of Kawasaki disease (n=29), Henoch-Schonlein purpura (n=15) and acute febrile illnesses (n=12). The production of the chemokines was assayed by ELISA. The plasma levels of IP-10 were markedly elevated in Kawasaki disease (538.6 +/-336.4 pg/mL) and acute febrile illnesses (417.1 +/-262.2 pg/mL) compared with in Henoch-Schonlein purpura (58.7 +/-95.7 pg/mL) (p<0.05). The MCP-1 levels were elevated in Kawasaki disease (443.0 +/-473.1 pg/mL) and acute febrile illnesses (328.6 +/-261.1 pg/mL) compared with in Henoch-Schonlein purpura (82.9 +/-79.0 pg/mL) (p<0.05). The Gro- levels were elevated only in acute febrile illnesses (134.3 +/-153.6 pg/mL) compared with in Kawasaki disease (31.8 +/-22.1 pg/mL) or Henoch-Schonlein purpura (29.4 +/-53.3 pg/mL) (p<0.05). According to these results, monocytes may play an important role in Kawasaki disease. In acute febrile illness-es, both monocytes and neutrophils may play an important role. By contrast, Henoch-Schonlein purpura may not be associated with the role of monocytes and neutrophils. Further studies using a larger number of cases are needed.
Acute Disease
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Adolescent
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Chemokines/*blood
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Child
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Child, Preschool
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Comparative Study
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Female
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Fever/*blood/*epidemiology
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Humans
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Infant, Newborn
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Korea/epidemiology
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Male
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Mucocutaneous Lymph Node Syndrome/*blood/*epidemiology
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Purpura, Schoenlein-Henoch/*blood/*epidemiology
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Research Support, Non-U.S. Gov't
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Risk Assessment/methods
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Risk Factors
7.Epidemiological study of hemorrhagic fever with renal syndrome in 1984-2003 in Weifang City, Shandong Province.
Fu-lian HAN ; Jian-ping LI ; Jian ZHOU
Chinese Journal of Epidemiology 2004;25(12):1096-1096
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Antibodies, Viral
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blood
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Child
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Child, Preschool
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China
;
epidemiology
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Female
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Hantaan virus
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isolation & purification
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Hemorrhagic Fever with Renal Syndrome
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epidemiology
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Humans
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Immunoglobulin G
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blood
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Male
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Middle Aged
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Prevalence
8.Serological surveillance on vaccine against hemorrhagic fever with renal syndrome.
Zhen-yu GONG ; Jing-qing WENG ; Zhi-ya ZHAO ; Yu-tu CHEN ; A-gen ZHU ; Gui-ming FU ; Chun-fu FANG ; Zhi-yong ZHU ; En-fu CHEN ; Chui-zhang WANG ; Wei WANG ; Lin-hai LEI ; Zhen WANG
Chinese Journal of Epidemiology 2004;25(5):400-402
OBJECTIVETo observe the serological and epidemiological efficacy of hemorrhagic fever renal syndrome (HFRS) vaccine in Zhejiang province.
METHODSImmunofluorescent antibody assay and Mcro-CPE method were used to test specific IgG antibody and the titer of neutralizing antibody.
RESULTSTwo weeks after the injection of the third dose, the sero-conversion rates by both immunofluorescent antibody test (IgG) and neutralization test were 100.0% (67/67) (95% CI: 96.3 - 100.0) and 44.4% (8/18)(95% CI: 22.0 - 69.0) with geometric mean titers (GMTs) 72.1 and 4.6 respectively. The rates of seroconversion of immunofluorescent antibody by immunofluorescence antibody assay (IFA) were 28.6%, 83.3%, 75.0%, 53.1%, 22.6%, 10.0% and 55.0% before reinforcement, two weeks, one year, one year and a half years, two years, three years and five years after reinforcement. The rates of neutralizing antibody seroconversion by the Mcro-CPE method were found as 14.8%, 55.6%, 35.0%, 31.3%, 26.0%, 10.0% and 50.0% respectively. We found some antibody dependent immunization enhancement phenomenon among the inoculated population, but further observation was needed.
CONCLUSIONHFRS vaccine was immunologically effective and the duration of serous antibody last long.
Adolescent ; Adult ; Antibodies, Viral ; blood ; China ; epidemiology ; Fluorescent Antibody Technique ; Hantaan virus ; immunology ; Hemorrhagic Fever with Renal Syndrome ; epidemiology ; prevention & control ; Humans ; Immunization Schedule ; Immunoglobulin G ; blood ; Male ; Middle Aged ; Neutralization Tests ; Vaccination ; Viral Vaccines ; immunology
9.Evaluation of a self-prepared anti-WNV-IgG diagnostic ELISA kit with a panel of serum samples collected from the people from areas in which West Nile fever is endemic.
Yu-Chun WANG ; Qiang SHAO ; Li-Ping ZHANG ; Wei ZHEN ; Xue-Min WU ; Xue-Zheng MA ; Yong ZHAO ; Kong-Xin HU
Chinese Journal of Virology 2014;30(5):549-553
In view of that there is no report of west Nile virus infection cases in our country, evaluation the self-prepared anti-WNV-IgG diagnostic ELISA kit should be employed with the establishment of the serum sample panel collected from the entry personnel. All individuals of entry personnel were traveled from epidemic area of infectious west Nile disease. In our study, the serum samples were both detected by self-prepared anti-WNV-IgG diagnostic ELISA kit and the FDA certified kits ,which are FOCUS West Nile Virus IgG Dxselect and Panbio Dengue IgG Capture ELISA kits. The self-prepared kit and FDA certified kits were compared and assessed simultaneously. Furthermore, the specificity, repeatability and stability of the kits were also evaluated. The results indicated that no significant difference of detective rates (35. 6% for self-prepared kit vs. 32.5% for FOCUS kit, χ2 = 3. 05, P > 0.05) and good consistency (Kappa = 0.8372) between the self-prepared kit and FDA certified kits. Also, the positive coincidence rate, the negative coincidence rate and the total coincidence rate were calculated as 91.18%, 95.34% and 92.66%, respectively. The laboratory self-developed kit presented similar quality as the counterpart kits with FDA certificate. The development of our self-prepared anti-WNV-IgG diagnostic ELISA kit will provide technical support for the prevention and control of west Nile virus entry.
Endemic Diseases
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Enzyme-Linked Immunosorbent Assay
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Humans
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Immunoglobulin G
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blood
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Reagent Kits, Diagnostic
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West Nile Fever
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epidemiology
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West Nile virus
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immunology
10.Clinical research of multisystem inflammatory syndrome in children.
Ruo Hang WENG ; Wei Ying ZHAO ; Ting Yan HE ; Xiao Lin LI ; Xiao Qing LI ; Dong Mei ZHAO ; Yun Kun HAN ; Ping ZENG ; Xue Mei TANG ; Xiao Chuan WU ; Li LIU ; Jun YANG
Chinese Journal of Pediatrics 2023;61(12):1086-1091
Objective: To analyze the clinical characteristics of children with multisystem inflammatory syndrome (MIS-C) associated with SARS-CoV-2 in China, and to improve the understanding of MIS-C among pediatricians. Methods: Case series study.Collect the clinical characteristics, auxiliary examinations, treatment decisions, and prognosis of 64 patients with MIS-C from 9 hospitals in China from December 2022 to June 2023. Results: Among the 64 MIS-C patients, 36 were boys and 28 were girls, with an onset age being 2.8 (0.3, 14.0) years. All patients suffered from fever, elevated inflammatory indicators, and multiple system involvement. Forty-three patients (67%) were involved in more than 3 systems simultaneously, including skin mucosa 60 cases (94%), blood system 52 cases (89%), circulatory system 54 cases (84%), digestive system 48 cases (75%), and nervous system 24 cases (37%). Common mucocutaneous lesions included rash 54 cases (84%) and conjunctival congestion and (or) lip flushing 45 cases (70%). Hematological abnormalities consisted of coagulation dysfunction 48 cases (75%), thrombocytopenia 9 cases (14%), and lymphopenia 8 cases (13%). Cardiovascular lesions mainly affected cardiac function, of which 11 patients (17%) were accompanied by hypotension or shock, and 7 patients (12%) had coronary artery dilatation.Thirty-six patients (56%) had gastrointestinal symptoms, 23 patients (36%) had neurological symptoms. Forty-five patients (70%) received the initial treatment of intravenous immunoglobulin in combination with glucocorticoids, 5 patients (8%) received the methylprednisolone pulse therapy and 2 patients (3%) treated with biological agents, 7 patients with coronary artery dilation all returned to normal within 6 months. Conclusions: MIS-C patients are mainly characterized by fever, high inflammatory response, and multiple organ damage. The preferred initial treatment is intravenous immunoglobulin combined with glucocorticoids. All patients have a good prognosis.
Male
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Child
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Female
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Humans
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Immunoglobulins, Intravenous/therapeutic use*
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Blood Coagulation
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COVID-19
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China/epidemiology*
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Connective Tissue Diseases
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Coronary Aneurysm
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Fever
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Systemic Inflammatory Response Syndrome/therapy*