1.Diagnostic challenges in fine needle aspiration cytology of salivary gland lesions
Fereshteh Ameli ; Asmazila Baharoom ; Nurismah Md. Isa ; Sharifah Noor Akmal
The Malaysian Journal of Pathology 2015;37(1):11-18
Background: Fine needle aspiration cytology (FNAC) has been widely accepted as a safe method
for diagnosis of salivary gland lesions and its accuracy is increased with increasing the experience
of the physician. This study was conducted to examine the sensitivity, specificity and accuracy of
FNAC of salivary gland lesions by cyto-histological correlation and to identify the discrepancies that
contribute to false diagnoses. Method: A retrospective study was carried out over a 7-year period
from 2003 to 2009 to review the cases of patients with salivary gland lesions who underwent FNAC
with histopathological confirmation. Results: A total of 101 cases had cytological correlation of
whom 76 (75.3%) were neoplastic (58.4% benign, 16.8% malignant) and 25 (24.7%) were nonneoplastic.
Pleomorphic adenoma (PA) was the most frequent benign neoplasm while adenoid cystic
carcinoma (ACC) was the most frequent malignant neoplasm. FNAC had a sensitivity of 80% and a
specificity of 98.8% for overall benign and malignant diagnoses and positive predictive and negative
predictive values of 92.3% and 96.4% respectively. The most common false negative cases were
pleomorphic adenoma. Conclusions: This study demonstrated that FNAC of the salivary gland is
a useful technique for diagnosis of salivary gland lesions. Combined with clinical and radiological
findings, it can provide a preliminary assessment on which management decision can be based.
2.Association study of TYMS gene expression with TYMS and ENOSF1 genetic variants in neoadjuvant chemotherapy response of gastric cancer
Khadijeh ARJMANDI ; Iman SALAHSHOURIFAR ; Shiva IRANI ; Fereshteh AMELI ; Mohsen ESFANDBOD
Journal of Pathology and Translational Medicine 2025;59(2):105-114
The present research was designed to study the associations between genetic variants of TYMS and ENOSF1 genes with TYMS and ENOSF1 gene expression in neoadjuvant chemotherapy response among patients with gastric cancer. Methods: Formalin-embedded and paraffin-fixed matched tumor and normal gastric cancer tissue samples from patients who received neoadjuvant 5-fluorouracil (5-FU) treatment were obtained. DNA and RNA were extracted for all samples. A 28-bp variable number tandem repeat (VNTR) at the 5' untranslated region of TYMS gene and rs2612091 and rs2741171 variants in the ENOSF1 gene were genotyped for normal tissue samples. The real-time polymerase chain reaction method was used to study the expression of ENOSF1 and TYMS genes in both normal and tumor tissues. Data were analyzed using REST 2000 and SPSS ver. 26.0 software programs. Results: A significant association between TYMS 2R3R VNTR genotypes and 5-FU therapy was found (p = .032). The 3R3R and 2R2R genotypes were significantly associated with increased and decreased survival time, respectively (p = .003). The 3R3R genotype was significantly associated with TYMS overexpression (p < .001). Moreover, a significant association was found between the rs2612091 genotype and treatment outcome (p = .017). Conclusions: This study highlights the impact of TYMS and ENOSF1 genes as predictive indicators for survival and response to 5-FU–based neoadjuvant chemotherapy in gastric cancer patients.
3.Association study of TYMS gene expression with TYMS and ENOSF1 genetic variants in neoadjuvant chemotherapy response of gastric cancer
Khadijeh ARJMANDI ; Iman SALAHSHOURIFAR ; Shiva IRANI ; Fereshteh AMELI ; Mohsen ESFANDBOD
Journal of Pathology and Translational Medicine 2025;59(2):105-114
The present research was designed to study the associations between genetic variants of TYMS and ENOSF1 genes with TYMS and ENOSF1 gene expression in neoadjuvant chemotherapy response among patients with gastric cancer. Methods: Formalin-embedded and paraffin-fixed matched tumor and normal gastric cancer tissue samples from patients who received neoadjuvant 5-fluorouracil (5-FU) treatment were obtained. DNA and RNA were extracted for all samples. A 28-bp variable number tandem repeat (VNTR) at the 5' untranslated region of TYMS gene and rs2612091 and rs2741171 variants in the ENOSF1 gene were genotyped for normal tissue samples. The real-time polymerase chain reaction method was used to study the expression of ENOSF1 and TYMS genes in both normal and tumor tissues. Data were analyzed using REST 2000 and SPSS ver. 26.0 software programs. Results: A significant association between TYMS 2R3R VNTR genotypes and 5-FU therapy was found (p = .032). The 3R3R and 2R2R genotypes were significantly associated with increased and decreased survival time, respectively (p = .003). The 3R3R genotype was significantly associated with TYMS overexpression (p < .001). Moreover, a significant association was found between the rs2612091 genotype and treatment outcome (p = .017). Conclusions: This study highlights the impact of TYMS and ENOSF1 genes as predictive indicators for survival and response to 5-FU–based neoadjuvant chemotherapy in gastric cancer patients.
4.Association study of TYMS gene expression with TYMS and ENOSF1 genetic variants in neoadjuvant chemotherapy response of gastric cancer
Khadijeh ARJMANDI ; Iman SALAHSHOURIFAR ; Shiva IRANI ; Fereshteh AMELI ; Mohsen ESFANDBOD
Journal of Pathology and Translational Medicine 2025;59(2):105-114
The present research was designed to study the associations between genetic variants of TYMS and ENOSF1 genes with TYMS and ENOSF1 gene expression in neoadjuvant chemotherapy response among patients with gastric cancer. Methods: Formalin-embedded and paraffin-fixed matched tumor and normal gastric cancer tissue samples from patients who received neoadjuvant 5-fluorouracil (5-FU) treatment were obtained. DNA and RNA were extracted for all samples. A 28-bp variable number tandem repeat (VNTR) at the 5' untranslated region of TYMS gene and rs2612091 and rs2741171 variants in the ENOSF1 gene were genotyped for normal tissue samples. The real-time polymerase chain reaction method was used to study the expression of ENOSF1 and TYMS genes in both normal and tumor tissues. Data were analyzed using REST 2000 and SPSS ver. 26.0 software programs. Results: A significant association between TYMS 2R3R VNTR genotypes and 5-FU therapy was found (p = .032). The 3R3R and 2R2R genotypes were significantly associated with increased and decreased survival time, respectively (p = .003). The 3R3R genotype was significantly associated with TYMS overexpression (p < .001). Moreover, a significant association was found between the rs2612091 genotype and treatment outcome (p = .017). Conclusions: This study highlights the impact of TYMS and ENOSF1 genes as predictive indicators for survival and response to 5-FU–based neoadjuvant chemotherapy in gastric cancer patients.