Objective To investigate the risk factors of restless leg syndrome (RLS) among male elders.Methods A case-control study between 148 old male RLS patients (case group) and 155 non-RLS male elders (control group) was conducted to investigate the risk factors of RLS.The general demographic data (age,education level,marital status,etc.),lifestyle (including physical exercise,smoking and alcohol) and past medical history were compared between two groups.Univariate analysis and non-conditional multivariate Logistic regression analysis was conducted by SPSS 13.0 software.Results In univariate analysis:there was significant difference in history of Parkinson disease between two groups [8.8％ (13/148)vs.3.2％ (5/155)] (P ＜0.05),but there was no significant difference in smoking,alcohol,physical exercise,history of diabetes mellitus and history of peripheral nerve disease between two groups (P ＞ 0.05).The serum creatinine in case group was higher than that in control group [(103.92 ± 32.07) μ mol/L vs.(85.35 ± 36.14) μ tmol/L] (P =0.000),and there was no significant difference in systolic blood pressure,diastolic blood pressure,hemoglobin,fasting blood glucose between two groups (P ＞ 0.05).Multivariate analysis showed that after adjusting for potential confounders,history of Parkinson disease and peripheral nerve disease,and serum creatinine was the independent risk factors of RLS among male elders.The values of OR(95％ CI) were 5.68(1.046-30.846),4.13(1.109-15.368),1.02(1.014-1.036),respectively.Conclusion Histories of Parkinson disease and peripheral nerve disease and serum creatinine are the independent risk factors of RLS among male elders.

Objectives To investigate the clinical feature of Parkinson's disease (PD) with restless leg syndrome (RLS) and the pathogenesis of RLS. Methods We conducted a cross-sectional and control study. The case group concluded 31 PD with RLS patients, meanwhile 39 PD patients were selected as the control group. Clinical history, clinical manifestations, complications and laboratory examinations were compared respectively between the two groups. Results All the RLS symptoms did not appear in RLS patients until the PD symptoms came out. Significant differences were found in complications such as swallow disturbance, constipation and illusion, when we compared the two PD groups (P ＜ 0.05 ). Compared with the PD or healthy group, the level of serum ferritin and the H-reflex latency of tibial nerve were significantly decreased in PD with RLS group ( P＜0.05). Conclusions Secondary RLS is a complication of PD.Deficiency of iron and decreased inhibition function of spinal cord may lead to the occurrence of RLS in PD patients. When their motor symptoms are serious and complications are more common, PD patients are more possible to have RLS symptoms.

Objective:To study the effect of injection bovine retinal soluble antigen (S-Ag)into anterior chamber(AC)in inducing immunological tolerance.Methods:S-Ag was extracted from fresh bovine retina and inoculated into the AC of Wistar rats,One week later,the S-Ag mixed with complete fruend's adjuvant(CFA)was injected into footpad intradermaly.The clinical symptoms and the pathological changes were observed.The IL-4 and IFN-? concentrations in the supernatant of splenic cell culture were measured by ELISA.Results:The incidence of EAU in pretreated group(group A) was markedly higher than that of positive controls(gropu B).There were histopathological changes in all rats with clinical symptoms.The IL-4 level of splenic cell culture in group A were significantly higher than those in group B ( P

In the present paper, the safety of several major plant growth regulators to environment, agricultural products and animals was discussed synthetically. Paclobutrazol is stable in physical and chemical properties with slow degradation, and is able to secondly control the plant growth obviously, but it is degradable and becomes mutagenic substance, it can be enriched in the soil and is able to bio-magnify. Uniconazole is quick degradable with less residue. Ethephon is potentially mutagenic to the animals, it is degradable and can produce residues which will be harmfulto animal body. Moreover, their residual quantity differs with different organs in a plant and their residue in the leafage of several plants is in a high level.

Objective This study is to analyze the fundamental frequencies of different speech materials . Methods 60 normal subjects (22 males ,38 females) were studied .When pronouncing /a/,/i/,/u/and the short paragraphs ,the fundamental frequencies were measured using Praat software .Results The analysis of the funda‐mental frequencies for different materials indicates that /i/and /u/were the highest while /a/was the next and the paragraphs were the lowest .For males ,the fundamental frequencies from high to low were:148 .47 Hz ,146 .77 Hz ,136 .33 Hz ,and 132 .85 Hz ;for females ,they were:257 .39 Hz ,250 .52 Hz ,239 .43 Hz and 222 .16 Hz .The experimental results showed that there was no significant difference between the fundamental frequencies of vowels between the two genders but there were significant differences when pronouncing different vowels among the female subjects .Conclusion When measuring fundamental frequencies ,reading short paragraphs can be more accurate and the vowel /a/is more useful in this purpose .

Objective To investigate the present situation and to analyze related factors of nursing practice environment among 22 public hospitals in Shanghai area.Methods We investigated 1 600 nurses from the 22 hospitals with general information questionnaire and the Chinese version of Practice Environment Scale,and also analyzed related factors by single and multiple factor analysis.Results 1 582questionnaires were taken back.49％ were dissatisfied with their working environment,and 28.1％ were evaluated to have nice practice conditions.Nurse concerned with workforce management and adequate resources were widespread.Logistic analysis indicated that hospital's level,the nurse's age,educational background and seniority were main factors influencing nurses' satisfaction.Nurse work in tertiary hospitals reported lower satisfaction.Conclusions More attention should be paid on improving nursing practice environment.

Objective : To learn the epidemiological characteristics of acute hepatitis B in Jiangxi Province from 2014 to 2021, so as to provide insights into perfecting hepatitis B prevention and control strategy. Methods: Data pertaining to acute hepatitis B was collected through the Chinese Disease Prevention and Control Information System. The temporal, spatial and population distributions of acute hepatitis B were analyzed using a descriptive epidemiological method. The trend in incidence of acute hepatitis B was analyzed using annual percent change (APC). Results: Totally 8 890 cases of acute hepatitis B were reported in Jiangxi Province from 2014 to 2021, with the reported incidence showing a tendency towards a decline (APC=-11.730%, P<0.05). The average annual reported incidence rate of acute hepatitis B was 2.42/105. Acute hepatitis B occurred all the year round, without obvious seasonal characteristics. The top three highest incidence rates were reported in Pingxiang City (7.12/105), Ganzhou City (3.12/105) and Fuzhou City (2.87/105). The lowest and highest incidence rates of acute hepatitis B were seen among residents aged under 10 years (0.30/105) and 20-29 years (3.86/105). The incidence rate of males was higher than that of females (3.25/105 vs. 1.55/105, P<0.05). Farmers were predominant population affected acute hepatitis B (5 764 cases, 64.84%). Conclusions The incidence of acute hepatitis B showed a downward trend in Jiangxi Province from 2014 to 2021, and the disease predominantly affected young males, farmers. Health education should be strengthened, and hepatitis B vaccination coverage should be promoted.

Objective To investigate the expression and significance of caveolin-1 in femoral nerve of diabetic patients with foot amputation. Methods Forty patients with foot amputation were assigned to 3 groups according to their duration of type 2 diabetes: group A ( ＜6 years＝, group B (6-10 years), and group C ( ＞10 years). Hematoxylin and eosin (HE) stain and Weil's stain were used to examine the femoral nerve. Silver staining was used to observe the axons and to count the nerve fiber density. The expression of caveolin-1 in Schwann cells of femoral nerve was tested by immunohistochemisty. Results There were evident progressive pathological changes in femoral nerve in the 3 groups. The variance of nerve fiber density in the 3 groups reached statistical significance ( P＜0. 05 ＝, the nerve fiber density showed negative correlation with HbA1C( r =-0. 792, P＜0. 01 ＝ and duration ( r=-0.592, P＜0. 01 ＝. The expression of caveolin-1 in Schwann cells of femoral nerve was positive in all the 3 groups and the variance with statistical significance (P＜0. 01 ), it was negatively correlated with HbA1C (r=-0. 762, P＜0. 01 )and duration (r=-0. 532, P＜0. 01 ), and it was positively correlated with nerve fiber density (r=0. 721, P＜0.01 ), the partial correlation coefficient of caveolin-1 and HbA1Cwas-0. 505 ( P＜0. 01 ).Conclusion In patients with diabetic foot amputation, caveolin-1 may play a role in the development of diabetic peripheral neuropathy and diabetic foot.

Objective To investigate the clinical value of transthyretin (TTR) from patients with early rheumatoid arthritis (ERA). Methods 58 patients with ERA , 34 patients with later RA (LRA) and 34 healthy control (HC) were included in the research. TTR was analyzed by ELISA, whose variance was analyzed. TTR density, disease activity score28 (DAS28) score and rheumatoid factor (RF) were tested, and their correlation with TTR was analyzed. Results Serum level of TTR with ERA significantly increased compared with that with LRA and HC (P < 0.05), no statistical significance with LRA group and HC. TTR level was no correlation with the number of swelling and tender joints, disease activity score 28, RF, ESR, CRP, anti-cyclic citrylinated peptide antibody and anti-keratin antibodies, hemoglobin, thrombocyte and albumin. Conclusion Serum level of TTR significantly increased with ERA patients, contributing to early diagnosis for RA.

BACKGROUNDIt has been proved that EphB4 and HIF-1α are closely related to the oncogenesis and development of lung cancer. The aim of this study is to investigate the biological significances of EphB4 and HIF-1α in lung cancer and their relationship with each other.

METHODSThe expression of EphB4 and HIF-1α was detected in 54 lung cancer tissues and 10 normal lung tissues as control by immunohistochemical method.

RESULTSEphB4 and HIF-1α proteins were detectable in 50.0% and 42.6% of all 54 lung cancer tissues respectively, which were significantly higher than those of the control (P < 0.05); the positive ratios and the levels of the expressions of EphB4 and HIF-1α proteins were closely related to gross types, differentiations and clinical stages (P < 0.05), but not to histological classification, age, sex and lymph node metastasis (P > 0.05). A highly positive correlation was observed between EphB4 and HIF-1α expression (P < 0.01 ).

CONCLUSIONSOverexpression of EphB4 and HIF-1α may play an important role in the pathogenesis, progression and malignant degree of lung cancer. Detection of EphB4 and HIF-1α expression might be helpful to predict prognosis of patients with lung cancer.