Objective To understand the achievement exchange and interpenetration situation between parasitological jour?nals and the journals of other disciplines. Methods The citing journals and cited journals of Chinese parasitological journals were analyzed. Three Chinese core journals namely Chinese Journal of Parasitology and Parasitic Diseases Chinese Journal of Schistosomiasis Control and Chinese Journal of Zoonoses were selected as the study objects. The number and citation times of citing and cited journals from 2002 to 2012 were collected from CNKI. These journals were classified according to China Journal Citation Reports-Expand 2013 and analyzed by the method of bibliometrics. Results The number of published pa?pers in Chinese Journal of Parasitology and Parasitic Diseases Chinese Journal of Schistosomiasis Control and Chinese Journal of Zoonoses were 1 160 1 541 and 2 494 from 2002 to 2012 respectively. The numbers of citing journals of the 3 above journals included by the citation reports were 496 547 and 592 respectively the total citation frequencies were 4 778 9 547 and 8 301 and the average citation frequencies per paper were 4.12 6.20 and 3.33 respectively. The numbers of the cit?ed journals were 532 407 and 659 respectively the total citation frequencies were 4 470 7 206 and 7 885 and the average citation frequencies per paper were 3.85 4.68 and 3.16 respectively. The top three disciplines of the citing journals and cited journals were medical and health basic science and agricultural sciences and the top three secondary disciplines belonged to medical and health were general medical and health preventive medicine and hygiene and clinical medicine. Conclusion There is an extensive exchange between parasitology journals and other journals which promotes the exchange between parasi?tology and other relevant disciplines.

Objective: To evaluate the efficacy of postoperative radiotherapy in the treatment of parotid gland carcinoma. Methods: Eighty-six postoperated patients with parotid gland carcinoma( 7 in stage Ⅰ, 28 in stage Ⅱ, 33 in stage Ⅲ and 18 in stage Ⅳ) were radiated by 60Co ?-ray or linear accelerator X-ray combined with electron beam. All patients were diagnosed by pathology and followed up for more than 5 years. Results: The five year survival rate and the local control rate were 73.3% and 87.2% respectively. Poor prognosis was observed in the cases with the neoplastic classification of undifferentiated carcinoma, sequamous cell carcinoma and malignant pleomorphic adenoma, but the better prognosis was obtained in the cases with acinic cell carcinoma and mucoepidermoid carcinoma. Poor prognosis was observed in the cases with stage Ⅲ and Ⅳ of clinical stage. Radiotherapy undertaken in 2 weeks after surgical operation gave higher 5- year survival ratio( 83.8%). The group given 51～60 Gy radiation showed 82.9% of five- year survival rate. Conclusion: The combination of surgery with radiation is effective in the treatment of parotid gland carcinoma.Radiation of 51～60 Gy 2 weeks after operation may result in better prognosis. Neoplastic type and clinical stage are important factors for prognosis.

We analyzed the sequence of vertebrate molecular marker genes, then we selected the mitochondrial DNA (mtDNA) 16S rRNA gene as marker gene. In order to detect four kinds of animal-derived ingredients, which including bovine, goat, pig and chicken. We utilized a pair of universal primers, designed four sets of species-specific microarray probes and two pairs of quality control probes. We optimized the PCR amplifications and hybridization conditions, therefore these four kinds of animal-derived ingredients could be rapid and accurate detected by this approach. The detection limits were all reaches 1 pg. We established the detection platform of these four kinds of animal-derived ingredients. This universal PCR-microarray assay provides a new method for the identification of animal-derived ingredients in the import-export field.
Animal Feed ; analysis ; Animals ; Cattle ; Chickens ; DNA, Mitochondrial ; genetics ; Food Contamination ; analysis ; Goats ; Meat ; analysis ; Microarray Analysis ; methods ; Mitochondria, Muscle ; enzymology ; genetics ; Polymerase Chain Reaction ; methods ; RNA, Ribosomal, 16S ; genetics ; Sensitivity and Specificity ; Swine

Objective To investigate the effects of Pixu Yihao Fang (PXYHF, No.1 Formula for Spleen Deficiency) on the expression of Glucose Transporter (GLUT1)protein (GLUT1) and mRNA in small intestine tissue of FD spleen-deficient rats and functional dyspepsia (FD) the intervention of Pixu I Fang.Methods 70 10-day-old male SD rat pups were randomly divided into normal control group (n=10), FD model group (single model, n=10), and spleen-deficient FD model group (double model, n=50).The normal control group received intragastric administration of 0.2 mL of 2% sucrose, while the single and double model groups were given 0.2 mL of 0.1 % iodoacetamide (IA)IA in 2 % sucrose, for 6 days.6 weeks after birth consecutive days, the Modified multiple platform method (MMPM) was also applied to establish double model for 14 days after week 6.These spleen-deficient FD rats were randomly divided into double model control group, domperidone group, PXYHF low-dose, medium-dose and high-dose dose groups, and were orally gavaged with 10 mL/(kg·d) of distilled water, 3.125 mg/(kg·d) of domperidone, 1.275, 2.55, 5.1 g/(kg·d) of PXYHF respectively for 14 days.Immunohistochemistry, Western-blot and RT-PCR methods were used to measure the GLUT1 protein expression in liver tissues.Results Compared with the normal group, average optical density of GLUT1 protein in small intestinal mucosal tissue, the expression of GLUT1 protein and GLUT1 mRNA in small intestinal tissue of double model group were decreased (P<0.05, P<0.01);Compared with double model group, average optical density of GLUT1 protein in small intestinal mucosa tissue, expression of GLUT1 protein and GLUT1 mRNA in small intestinal tissue of low-dose PXYHF were increased (P<0.01).Conclusion The expression of GLUT1 gene and protein decreased in the FD rats with spleen deficiency, and Pixu Yihao Fang could increase the expression of GLUT1 gene and protein.

Objective To investigate the intervention of Pixu Fang 1 (Spleen-deficiency Formula 1) to the protein and mRNA expressions of myosin light chain (MLC) in gastric tissue in rats with functional dyspepsia (FD)-spleen deficiency pattern(SDP).Methods SD rats (n =60,aged 7 d) were randomly divided into normal group (n =10) and FD-SDP model group (n =50).The normal group was orally given 2％ sugar solution and FD-SDP model group was given 0.1％ iodoacetamide sugar solution in a dose of O.2 mL each rat a day for 6 d.The FD-SDP model group was fed with normal forage until rats aged 6 weeks and additionally given modified multiple platform method (MMPM) continuously for 14 d.After modeling,FD-SDP model group was divided randomly into model group (distilled water,10 mL/kg · d),domperidone group (3.125 mg/kg · d),low-dose Pixu Fang 1 group (low-dose group,1.275 g/ kg · d),mid-dose Pixu Fang 1 group (mid-dose group,2.550 g/kg · d) and high-dose Pixu Fang 1 group (high-dose group,5.100 g/kg · d,each n =10),and all groups were given orally corresponding medicinal for 14 d.The protein and mRNA expressions of MLC in gastric tissue were detected by using immunohistochemistry technique,Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with normal group,MLC protein expression decreased in model group detected by immunohistochemistry technique and Western blotting,and MLC mRNA expression decreased in model group detected by RT-PCR (P ＜0.01).Compared with model group,MLC protein expression increased in low-dose group,mid-dose group and high-dose group detected by immunohistochemistry technique (P ＜ 0.05,P ＜ 0.01).MLC protein expression increased in mid-dose group and high-dose group detected by Western blotting (P ＜ 0.O1).MLC mRNA expression increased in high-dose group detected RT-PCR (P ＜ 0.01).Conclusion There are decreases of protein and mRNA expressions of MLC in FD-SDP rats,and Pixu Fang 1 can up-regulate these expressions.