Objective To establish alcoholic liver disease (ALD) model of rats. Method Rats were given wine by ig for 13 weeks. ALD were assessed by the level of serum glutamic-pyruvic transaminase (ALT), glutamic oxalacetic transaminase (AST), malonaldehyde (MDA) and pathological changes of liver. Result Over-dose wine can increase the level of serum ALT, AST, and MDA, decrease the level of serum albumin (ALB), and induce hepatic steatosis. Conclusion Alcoholic liver disease model of rats can be established by long-time and over-dose wine. The model can be applied in the research of ALD.

Objective To investigate the diagnostic value of Enzymes linked immunosorbent assay(ELISA)detection of anti phospholipase A2 receptor antibody(anti-PLA2R)in idiopathic membranous nephropathy patients.Methods All articles published from January 2012 to April 2017 about ELISA test on anti phospholipase A2 recepeor antibody as a maker for IMN were collected, and the quality of the studies was evaluated by using the guidelines of the updated Quality Assessment of Diagnostic Accuracy Stud-ies(QUADAS-2)tool.According to the inclusion criteria and exclusion criteria,the research objects were selected and the data were extracted,and the analysis was carried out by Meta-Disc1.4 software.The results are summarized on the basis of sensitivity,speci-ficity,and summary receiver operating characteristic curve(ROC curve).Results Ten articles were selected according to inclusion criteria and 1517 cases were included in the study.The combined sensitivity was 0.68[95% CI(0.64,0.70)];the merge specificity was 0.92[95% CI(0.90,0.95)],and the subjects'working curve showed AUC 0.807 4.Conclusion The detection of anti-PLA2R by ELISA is effective in the diagnosis of idiopathic membranous nephropathy,which can be used as an effective supplement for the diagnosis of idiopathic membranous nephropathy.