Objective To evaluate the safety and efficacy of the interventional techniques for the management of hepatic artery stenosis (HAS) after orthotopic liver transplantation (OLT).Methods Seven patients diagnosed with HAS after OLT were treated with interventional procedures. Three (patients) presented with biliary ischemic complications, 1 with hepatic abscess, and 3 with elevation of liver enzymes and bilirubin levels. Interventional techniques included transcatheter hepatic arterial thrombolysis with a low dose of urokinase (UK), and stent placement in the stenotic segment of the hepatic artery. All patients were followed up regularly by duplex ultrasound scan after discharge.(Results) HAS with over 70 % degree at the anastomosis was found in 3 patients. Complete occlusion of the proper hepatic artery was found in 4 patients, and the hepatic artery flow was re-established in these patients at 3 to 7 days after transcatheter hepatic arterial thrombolysis. However, an over 90 % degree of stenosis at the anastomosis was seen in 4 cases. Stent placement in the stenotic segment of the hepatic artery was performed successfully in 7 patients, and significant improvement of liver (function) was seen in these patients at 2 weeks after the procedures. No complications related to the procedure occurred. The 7 patients had a good clinical course with normal graft function during a mean follow-up of 9 months (4 to 18 months), and patent hepatic artery flow was identified by follow-up Doppler ultrasonography.Conclusion Transcatheter endovascular interventional techniques are safe and effective methods for treatment of HAS after OLT.

Objective To investigate the frequency, patterns of the angiographic findings and risk factors for development of complications after transcatheter arterial chemoembolization (TACE) via the inferior phrenic artery (IPA).Methods One hundred and sixty eight patients with hepatic tumor fed by the IPA underwent a total of 226 procedures of TACE of the IPA, with combined routine TACEO(88.7%) or without (11.3%) the combination of routine TACE of the hepatic artery. TACE of the malignancy was performed with an emulsion of iodized oil and anti-cancer agents and then followed by gelatin sponge particle injection. The complications were assessed on clinical observation, laboratory tests, and computed tomographic (CT) images after TACE of the IPA; and finally were correlated with the angiographic findings of the IPA. Results The tumor supplied by IPA was noted at initial TACE in 25 (14.9%), at successive TACE in 97 (57.8%), after percutaneous tumor ablation guided by ultrasound in 28 (16.7%), and after hepatic surgery in 18 (10.7%) cases. The abnormal findings on arteriography showed enlargement of the IPA with tumor staining in all cases, dense non-tumor staining in 61(36.3%), artery-to-artery anastomosis in 62(36.9%),arteriovenous shunts in 12(7.1%) cases. 96.4% of these patients complained of shoulder pain during TACE of the IPA. The cumulative complication rate after the procedure was 8.9%,including hiccup in 13 cases (5.8%), chest pain with cough and mild hemoptysis in 9 (4.0%), phrenoparalysis in 8 (3.5%), angina with abnormal ECG in 4 (1.8%), increasing of CPK, AST, and ALT significantly in 6 (2.7%), transient myeloparalysis in 2 (0.9%), and subphrenic abscess in 2 cases (0.9%). Two patients with the subphrenic abscess were successfully treated with percutaneous catheter drainage and antibiotics. The symptoms of these mild complications subsided with symptomatic treatment.Conclusions Complications of TACE via the IPA occur in about 9% of the patients and these complications do not worsen the patients survival. IAP angiographic abnormalities such as arteriovenous shunts, artery-to-artery anastomoses, dense non-tumor contrast staining,are important risk factors for complications of TACE of the IPA.

Transcatheter internal iliac arterial embolization has become a first-choice treatment for control of life-threatened bleeding associated with pelvic tumors or obstetric disorders.Recently,uterine arterial embolization(UAE)has offered a successful alternative to surgery in the treatment of uterine fibroid.Clinical failure rates for this procedure was reported from 4% to 19% in literature,probably due to the existence of collateral flow to the pelvic lesions,especially the ovarian arteries(OA)being the most important source.Furthermore,the anastomosis between the ovarian and uterine arteries has been suggested as the pathway for nontarget embolization of the ovaries causing premature menopause.The OA are simple paired vessels and they usually arise from the anterolateral part of the abdominal aorta at the level of the second lumbar vertebra.Variations in origin of OA occur in 2%-10% with diameter smaller than 1.1 mm and routinely not identified on conventional aortography.However,in the presence of a uterine or other pelvic diseases,the OA become dilated as the result of pathologic blood supply demand.OA supply to the pelvic lesions are more frequently found in patients with large fundal fibroids,history of the pelvic surgery,post embolization of the uterine arteries,and dysplasia of uterine artery.In patient with one or more of these predisposing factors,the extent of OA supply to the pelvic lesions should be assessed using pigtail catheter pelvic aortography with the tip at the level of the renal arteries,followed by selective OA catheterization.In case of identified OA supply to the pelvic lesions,superselective embolization of the OA should be considered,using particles with diameters larger than 500 ?m.Proximal OA embolization,in combination with bilateral UAE,may significantly increase the risk of iatrogenic ovarian dysfunction.When necessary in a premenopausal patient,OA embolization should be performed unilaterally and as near as possible to the lesions,and the procedure is performed only with specific informed consent.

Objective To investigate the effects of high concentrations of iodide exposure on mitochondrial superoxide production,cell viability and cell damage in the thyroid of metallothionein Ⅰ/Ⅱ knockout (MT-Ⅰ/Ⅱ KO) mice and corresponding wild type (WT) mice.Methods Thyroid cell suspension of six to eight weeks old healthy male MT-Ⅰ/Ⅱ KO mice and WT mice were prepared.The thyroid cells were treated with high concentrations (10-4,10-3,10-2 mol/L) of potassium iodide(KI),or 10-3 mol/L hydrogen peroxide(H2O2) for 2 hours,respectively.Cell viability was evaluated with methyl thiazolyl tetrazolium(MTT) assay.Lactate dehydrogenase (LDH) level in cell culture medium was detected by enzyme-linked immunosorbent assay(ELISA).Mitochondrial superoxide production in the thyroid cells was measured by flow cytometry using a fluorescent probe,mitochondrial superoxide(MitoSOX).Results Compared to the control group[(100.00 ± 0.00)％,(100.00 ± 0.00)％],the cell viability of 10-4,10-3,10-2 mol/L KI and 10-3 mol/L H2O2 exposure groups were significantly decreased in the thyroid cells of both WT [(73.63 ± 2.05)％,(72.41 ± 2.26)％,(69.63 ± 2.29)％,(44.90 ± 2.93)％] and MT-Ⅰ/Ⅱ KO mice[(65.40 ± 2.39)％,(64.51 ± 2.27)％,(61.48 ± 2.33)％,(40.80 ± 2.76)％,all P＜ 0.05].Compared to the control group [(1 995.28 ± 30.52),(2 004.96 ± 19.71)U/L],significantly increased LDH activities were detected in the thyroid cells of WT [(2 809.22 ± 156.53),(2 850.80 ± 137.83),(2 920.45 ± 152.92),(4 487.49 ± 130.67)U/L] and MT-Ⅰ / Ⅱ KO mice [(3 261.06 ± 120.44),(3 474.19 ± 142.15),(3 597.08 ± 150.86),(4 706.64 ± 148.57)U/L,all P ＜ 0.05].Compared to the control group (26.49 ± 7.66,37.11 ± 8.48),the MitoSOX red fluorescence intensities of 10-2 mol/L KI and 10-3 mol/L H2O2 groups were significantly increased in WT mice(58.96 ± 5.11,87.95 ± 4.25) and MT-Ⅰ/ⅡKO mice(71.21 ± 5.55,99.76 ± 4.42) by flow cytometry (all P ＜ 0.05).Compared to the thyroid cells in WT mice,significantly decreased cell viability (all P ＜ 0.05),significantly increased LDH activity(all P ＜ 0.05) and significantly increased MitoSOX red fluorescence intensity by flow cytometry (all P ＜ 0.05) were detected in the thyroid cells of MT-Ⅰ/Ⅱ KO mice following treatment with KI or H2O2.Conclusions High concentrations of iodide (10-2 mol/L) and 10-3 mol/L H2O2 may lead to significant increase of mitochondrial superoxide production and LDH activity,decrease of cell viability in both WT and MT-Ⅰ / Ⅱ KO mice.More significant increase of superoxide production is detected in MT-Ⅰ / Ⅱ KO mice,indicating the potential protective role of metallothionein in the thyroid cells of WT mice.

mbus in IVC. Interventional treatment can prolong the survival time and promote the quality of life.

90%) of CA. The stenotic segments were dilated and stented during the same session. One patient with balloon expandable Palmaz stent placed in the proximal celiac artery, the another with 2 wallstents deployed in the CA trunk. The postprocedural arteriograms showed good dilation of the lesions with immediate improvement of CA blood flow. Follow-up Doppler ultrasound scans showed normal flow patterns in the CA. Three months after the procedures, their upper gastrointestinal symptoms had resolved and regained body weights. They remained well and free of symptoms, at 16 months and 26 months follow-up, respectively, after the procedure.Conclusions CA stenosis can successfully be treated with angioplasty and stenting.

Objective To analyze the causes of recurrent hemoptysis after interventional treatment and to provide the reference to improve the interventional radiology technique in dealing with the hemoptysis.Methods From Oct.2000 to Oct.2008,repeated interventional procedure was carried out in 22 patients with recurrent hemoptysis after initial interventional treatment.After the bronchial arterial angiography and collateral branch arterial angiography were performed,embolization of diseased arteries with gelatin sponge particle,PVA particle or microcoil was conducted.Results Of 22 patients,re-canalization of the bronchial arteries was seen in 4,blood supply from additional bronchial artery other than from the already embolized bronchial artery in 3,and blood supply from the collaterals in 15.Technical success of the second interventional treatment was achieved in all the 22 cases.Bleeding ceased immediately after the second interventional treatment and no recurrent hemoptysis occurred during the follow-up period of 3 months to 4 years.Conclusion Complete embolization of all arteries related to the hemoptysis together with actively dealing with the primary disease can effectively improve the successful rate of interventional treatment for the massive hemoptysis.

Objective To connect the content of clinical immunology and inspection with network,so that it is more convenient for medical students to understand and study this course,and the learners’interest in learning is aroused.Methods FrontPage 2003 web page authoring software,as well as animation software(flash) and picture editing software(Photoshop) were used to make a vivid image of the teaching content be shown through the web page,and effectiveness evaluation was done through peer review and students survey about the software.Results Using FrontPage to create web pages and change knowledge into learning pattern.got good results in studetns’self-study,and the"online self-test"section was added,which would more effectively check study effect.There were good student feedbacks.Conclusion Applying this learning pattern to study will play a very good role in deepening students’understanding of curriculum,improving their study efficiency and stimulating their interest in learning.

Objective To assess the efficacy and safety of interventional techniques for the management of symptomatic portal vein (PV) and superior mesenteric vein (SMV) thrombosis. Methods Six patients with thrombosis of the PV and SMV were treated by transjugular intrahepatic portosystemic (TIPS) approach. Contrast PV SMV venography was carried out to verify the access to the PV branch. Then a 8F large bore catheter was used to aspirate thrombus. A J shaped guide wire and pig tail catheter were used to fragment the thrombus. Local thrombolysis with urokinase (UK) was delivered through a catheter in the SMV. An 4 French multiple side hole catheter was put into the SMV and UK was continuously infused for 3 to 13 days.Results The procedure was successful in all cases. One patient died of intra abdominal sepsis 12 days after. Five patients were followed up by color Doppler ultrasonography for 4～36 months with confirmed patency of the PV and SMV in all these patients. Conclusions This procedure is both safe and effective in the treatment of symptomatic PV and SMV thrombosis.

Objective To evaluate the safety and efficacy of transcatheter arterial embolization (TAE) of massive polycystic liver disease (PLD).Methods A total of 21 patients with symptomatic PLD were enrolled.The patients consisted of seventeen women and four men (aged 36-64 years,mean age,49 years).Transcatheter superselective embolization was performed with the mixture of N-butylcyanoacrylate (NBCA) and iodized oil.All patients underwent contrast enhanced computed tomography (CT) of the liver before TAE and at every 3 months for the first half year after TAE,and at 6-monthly intervals thereafter.Laboratory data,including routine blood tests and liver enzymes,were collected.T test was used for statistics.Results All procedures were successful without serious complications.There was no obvious improvement during the first three months.At follow-up of 6-12 months,symptoms notably improved in 18 of 21 patients,and these patients experienced further relief of the symptoms in the follow-up period.TAE failed to benefit in 3 patients,but there were no complaints of worsening of the symptoms.At follow-up CT,the total liver volume and total intra-hepatic cyst volume decreased significantly (t =6.75,7.73,P ＜0.01)compared with pre-TAE in 18 patients at 12 months after TAE.The total liver volume decreased from (8270 ± 3016) cm3 to (6120 ± 2680) cm3 and the total intra-hepatic cyst volume decreased from (7120 ±3070) cm3 to (4560±2488) cm3.Mild elevation of the liver enzymes returned to the normal range within 1 month in all patients.Conclusions It is suggested that transcatheter super selective embolization with the mixture of NBCA and iodized oil is a safe and effective treatment for PLD patients.This technique is a supplemental option for traditional therapy.