1.Prevalence of Cryptosporidium spp. infections in large - scale pig farms in north of Yangtze River in Anhui Province
Wen-Chao LI ; Hui-Hui YANG ; Zhen-Zhen KAN ; Yun-Long YANG ; Yue-Yang SUN ; You-Fang GU ; Hui-Liang CHEN
Chinese Journal of Schistosomiasis Control 2018;30(4):420-423
2.Progress of researches on the allergens Ara h 1, Ara h 2 and Ara h 3 from peanut.
Shengjuan JIANG ; Zhengyi ZHOU ; Yujun SUN ; Songhua WANG
Journal of Biomedical Engineering 2010;27(6):1401-1405
Peanut is one of the most popular foods in the world due to its high nutrition; however, it contains multiple seed storage proteins which are identified as allergens and hence are the most common cause of life-threatening, IgE-mediated anaphylaxis among the hypersensitive individuals. Three peanut proteins, Arachis hypogaea allergy 1, 2, 3 (Ara h 1, Ara h 2 and Ara h 3), which have the common biochemical characteristics like resistance to proteases and heat, are considered as the major allergens because they are recognized by serum IgE from a peanut-allergic patient population. The linear IgE-binding epitopes in the allergens lay the foundation of the anaphylaxis in the peanut-allergic individuals. Peanut allergy is often a life-long problem, so many investigators are focusing on decreasing clinical reactivity. In this review, the latest advances in the researches on biochemical characteristics, structure and function of the three major allergens were described and particular attention was given to the immunity properties of the three allergens. The future research directions were also discussed.
2S Albumins, Plant
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chemistry
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genetics
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immunology
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Animals
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Antigens, Plant
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chemistry
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genetics
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immunology
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Arachis
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chemistry
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DNA
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genetics
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Glycoproteins
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chemistry
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genetics
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immunology
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Humans
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Immunoglobulin E
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genetics
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Plant Proteins
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chemistry
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genetics
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immunology
3.Effects of surface treatment and adhesive application on shear bond strength between zirconia and enamel.
Yinghui LI ; Buling WU ; Fengyang SUN
Journal of Southern Medical University 2013;33(3):420-423
OBJECTIVETo evaluate the effects of sandblasting and different orthodontic adhesives on shear bond strength between zirconia and enamel.
METHODSZirconia ceramic samples were designed and manufactured for 40 extracted human maxillary first premolars with CAD/CAM system. The samples were randomized into 4 groups for surface treatment with sandblasting and non-treated with adhesives of 3M Transbond XT or Jingjin dental enamel bonding resin. After 24 h of bonded fixation, the shear bond strengths were measured by universal mechanical testing machine and analyzed with factorial variance analysis.
RESULTSThe shear bond strength was significantly higher in sandblasting group than in untreated group (P<0.05) and comparable between the two groups with the adhesives of Transbond XT and dental enamel bonding resin (P>0.05).
CONCLUSIONThe shear bond strength between zirconia and enamel is sufficient after sandblasting regardless of the application of either adhesive.
Adhesives ; Adolescent ; Adult ; Computer-Aided Design ; Dental Enamel ; Humans ; In Vitro Techniques ; Molar ; Resins, Synthetic ; Shear Strength ; Surface Properties ; Young Adult ; Zirconium
4.Effect of clopidogrel on plasma protein binding rate of ginsenosides: a liquid chromatography-mass spectrometry-based study.
Shi-Tang MA ; Guo-Liang DAI ; Wen-Zhu ZHAO ; Bing-Ting SUN ; Wen-Zheng JU ; Heng-Shan TAN
Journal of Southern Medical University 2015;35(1):109-112
OBJECTIVETo investigate the effect of clopidogrel on the binding rate of ginsenosides with rat serum proteins (RSA).
METHODSEquilibrium dialysis and liquid chromatography-mass spectrometry were employed to quantify the concentration of ginsenoside Rg1 and Rb1. The protein-binding rates of Rg1 and Rb1 in the presence or absence of clopidogrel (1.0 mg/L) were determined. A molecular simulation model (consisting of homology modeling and molecular docking interaction) was used to reveal the target protein-compound interactions.
RESULTSThe binding rates of ginsenosides Rg1 (0.4, 1.0, and 2.0 mg/L) with RSA were (30.16∓2.82)%, (33.42∓4.21)%, and (34.61∓3.42)%, and those of and Rb1 were (50.13∓2.34)%, (51.23∓3.23)%, and (53.11∓3.26)%, respectively. In the presence of clopidogrel, the binding rates of Rg1 decreased to (22.13∓2.72)%, (21.42∓3.22)%, and (25.45∓3.52)%, and those of Rb1 to (40.13∓3.24)%, (41.25∓4.15)%, and (43.11∓3.31)%, receptively. The molecular docking suggested that these compounds competed to bind with RSA.
CONCLUSIONClopidogrel can competitively bind to RSA with ginsenosides to lower the plasma protein binding rates of ginsenosides.
5. Application value of contrast-enhanced ultrasound in differential diagnosis of complex renal cysts and clear renal cell carcinoma with cystic change
Pei SUN ; Beijian HUANG ; Liyun XUE ; Cuixian LI ; Fengyang ZHENG ; Lixia YAN ; Wenping WANG
Chinese Journal of Ultrasonography 2019;28(12):1045-1049
Objective:
To investigate the value of contrast-enhanced ultrasound(CEUS) in differential diagnosis of complex renal cysts and clear renal cell carcinoma with cystic change(CRCCC).
Methods:
The ultrasonographic datas of 82 lesions in 82 patients with complicated renal cysts or CRCCC confirmed by pathology were analyzed. The characteristics of conventional ultrasound and CEUS were observed and evaluated. The lesions were graded according to Bosniak classification criteria.
Results:
Pathological examination showed that 36 cases were complicated renal cysts and 46 cases were CRCCC. Routine ultrasound showed there were 9 cases (25.0%) with cystic masses and 27 cases (75.0%) with solid and cystic masses in complex renal cysts, of which 14 cases (38.9%) could detect color flow signals. In CRCCC, 2 cases (4.3%) were with cystic masses and 44 cases (95.7%) were with solid and cystic masses, of which 33 cases (75.0%) could detect color flow signals. CEUS showed that only 18 cases (50.0%) of the complex renal cysts showed enhancement of cystic wall or septum, with equal or low enhancement at the peak, 9 cases (50.0%) accompanied by decrease of renal cortex, 35 cases (97.2%) had thin and regular cystic wall, no enhancement of cystic wall in all lesions, and 33 cases (91.7%) had septal thickness less than 1 mm. Forty-five cases (97.8%) of CRCCC showed enhancement of cystic wall or septum, 40 cases (88.9%) showed equal or high enhancement at peak, 30 cases (66.7%) were faster than the decrease of renal cortex, 37 cases (80.4%) showed uneven thickness of cystic wall, 24 cases (52.2%) showed enhancement of cystic wall nodules, and 28 cases (60.9%) showed uneven thickness of septum. After CEUS, 33 cases (91.7%) of complex renal cysts were classified as grade Ⅰ and Ⅱ, while 42 cases (91.3%) of CRCCC were classified as grade Ⅲ and Ⅳ.
Conclusions
The CEUS manifestations of complex renal cysts are different from those of CRCCC. The application of Bosniak criteria in CEUS is helpful for the differential diagnosis of complex renal cysts and CRCCC.