Objective To explore the cinical significance of Plasma Endothelin-1(ET-1) and Nitrous Oxide(NO) in the children associated with left to right shunt congenital heart defect(CHD) with Pulmonary Hypertension(PH).Methods 42 children associated with left to right shunt congenital heart defect with pulmonary hypertension(as patient's group),26 children associated with left to right shunt congenital heart defect without pulmonary hypertension(as heathly group),20 children had the checkup(as control group).Plasma ET-1 and NO2+/NO3+ was cletected in all groups by radioimmunoassay and nitric acid-restored method.Results ET-1 of patient's group was significantly higher than that of control group(P0.05);NO2+/NO3+ of that was significantly lower control group(P

Objective: To understand the pathogen spectrum composition of non-bacterial respiratory infections in Pinghu City, Zhejiang Province, so as to provide insights for the prevention and control of respiratory infectious diseases. Methods: A total of 592 throat or nasopharynx swab samples were collected from fever patients in Pinghu First People's Hospital from Jamuary 2021 to November 2022. Multiple real-time fluorescence quantitative polymerase chain reaction was used to detect the nucleic acids of rhinovirus (RhV), respiratory syncytial virus (RSV), parainfluenza virus (PIV), adenovirus (ADV), metapneumovirus (MPV), coronavirus (CoV), Boca virus (Boca), enterovirus (EV), influenza virus (Flu), chlamydia pneumoniae (CP) and mycoplasma pneumoniae (MP). The detection rates of pathogens and mixed infections in different age groups and seasons were analyzed. Results: A total of 212 samples were tested positive for at least one pathogen's nucleic acid from 592 samples, with a total detection rate of 35.81%. The detection rates of RhV (9.80%), PIV (7.26%), Flu (6.76%), RSV (4.39%) and CoV (3.72%) were relatively high. The detection rates were higher among patients at ages of 0 to 2 years and 3 to 17 years than among patients at ages of 18 to 59 years, and in autumn than in spring and winter (all P<0.05). Twenty-three samples were infected by mixed pathogens, accounting for 3.89%. The mixed infections were all detected two pathogens, with PIV, CoV, RhV, and ADV predominant. Conclusions From 2021 to November 2022, the main pathogens of non-bacterial respiratory infections in Pinghu City were RhV, PIV, FLu, RSV and CoV, and there were mixed infections. It is necessary to strengthen the prevention and control of respiratory infection in infants and children.

Objective To investigate the inhibitory effect of ethosuximide,an antiepileptic drug,on sevoflurane-induced emergence delirium in neonatal rats.Methods Twelve SD rats (17-23 g) at postnatal day 9-11 were randomly divided into ethosuximide group (4 mg/100 μl,intraperitoneal injection,n=6) and saline group (100 μ1,intraperitoneal injection,n =6).Then the pups were treated with 1％ sevoflurane for 10 minutes and agitated behaviors was observed using PAHBs scoring method for every 2 minutes.Ten more pups were randomly divided into two groups as previously described.Then the pups were treated with 1％ sevoflurane for 10 minutes and EEG power spectrum of frontal lobe was monitored for every 2 minutes.Results Both PAHBs scores and EEG power spectrum at 2 min,4 min,6 min,8 min and 10 min were significantly decreased in ethosuximide group when comparing with saline group under 1％ sevoflurane inhalation (P ＜ 0.05).Conclusion Ethosuximide can decrease frontal lobe EEG power spectrum in sevoflurane-induced agitated rats and inhibit sevoflurane-induced emergence delirium in neonatal rats.