Objective To study whether the silica nanoparticles can be used as a biomacromolecular carrier of oral drugs.Methods Studyed the influence of the amount of calcium ions on nano silica nanoparticles morphology,the encapsulation rate of silica nanoparticles on proteins or peptides in the presence of different calcium ions,the situation of proteins or peptides released from silica nanoparticles,enzymolysis of protein packaged by silica, silica nanoparticles labeled peptides with 125 I released in mice.Results Experiments showed that the synthetic silica nanoparticles packaged with peptides could smoothly reach the small intestine through the gastric acid in mice, it had potential to be biomacromolecular carrier of oral drug. Conclusion Proteins or peptides is easy to release in alkaline condition,but release few in acidic condition of pH2 (PH of gastric acid).The silica nanoparticles prepared have the potential to be oral drug carrier.

Objective By finding out the Chinese official pharmacy talent training and education model in Sui and Tang dynasties,we can used it in the cultivation of contemporary talents in pharmacy.Methods pharmacy education in CNKI,Sui and Tang history books,Chinese herbal medicine and legal literature were collected and categorized in training organizations,education and evaluation three aspects.Results In the Sui and Tang dynasties,pharmacy institutions included imperial pharmacies,drug possession Bureau and the Department of imperial medicine disciplines.Meanwhile,there were relevant regulations in education,teaching and quality assessment.According to the traditional theory,practice and correlation characteristics,focusing on the connection of clinical pharmacy practice courses and lessons,the government trained a large number of professional talents in pharmacy.Conclusion In the Sui and Tang dynasties,Chinese material medical attaches great importance to the comprehensive quality of talents cultivation,which is worthy for modern reference.

Objective:To statistics and analyze the detection rate of 5 kinds of common respiratory virus specific IgM in children with respiratory tract infection to guide the clinical.Methods: 1 609 cases of children with respiratory tract infection in department of Pediatrics in our hospital were collected in 2012 from 1 to 12 months.Serum was collected to detect the specific IgM of respiratory syncytial virus (RSV),adenovirus (ADV),influenza A virus (IVA),influenza B virus (IVB) and parainfluenza virus ( PIV) by method of indirect immunofluorescence.Results:263 positive cases were detected from 1 609 cases ( 16.35%) , and the positive rates of IVB was the highest (8.45%) with a total of 136 cases,followed by the rest of IVA 63 cases(3.92%),PIV 62 cases (3.85%),ADV 44 cases(2.73%) and RSV 42 cases(2.61%).The positive detection rate was different in children of different age groups,and the difference was statistical significant (χ2=134.822,P<0.01).Positive detection rate was gradually increased along with the age growth,and the detection rate of school-age children was the highest (34.55%);The comparison differences of IVB ,PIV,IVA positive detection rate between different age stages were significant (χ2=102.660 ,86.145 ,39.791 ,P<0.05 ).The detection rate of IVB and IVA was the highest in school-age children,20.00%and 8.64%respectively.The detection rate of PIV was the highest (12.07%) in preschool children.The positive rates of different respiratory virus specific IgM had their own characteristics in different months .IVB appeared two positive peaks in March and October with the rate of 20.15%and 11.11%respectively;IVA appeared two positive peaks in March and May with the rate of 10.27% and 11.81% respectively;PIV and RSV , the detection rate of which was the highest ( 7.98%and 6.46 respectively ) in March increased gently from January to June ,and then declined steadily.The detection rate of ADV was the highest (6.19%) during three months of March ,April and May.Conclusion:The positive rate of 5 kinds of respiratory virus specific IgM was 16.35%,especially the detection rate of IVB was the highest.Positive detection rate of respiratory virus specific IgM was gradually increased along with the age growth;The detection rate of IVB and IVA was the highest in school-age children ,while PIV in preschool children.5 kinds of respiratory tract virus own respectively prevalent characteristics in 2012.

Objective · To compare urine sample collection methods for measuring urinary concentrations of phenolic endocrine disrupting chemicals including bisphenol A (BPA),triclosan (TCS),and 4-n-nonylphenol (4-n-NP) in pregnant women.Methods· Urine samples were collected from women at late pregnancy by two methods:urine catheter and collection bag (n=176),urine collecting containers made of polypropylene (PP) (n=642).Urinary concentrations of BPA,TCS and 4-n-NP were measured with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)method.Confirmation experiment used PP containers,urine catheter and collection bags,as well as low-density polyethylene (LDPE) tubes and glass containers as both negative controls to collect urine samples from each of the 5 women.Results · Geometric mean (GM) of urinary BPA concentration collected by urine catheter and collection bags was 82.5 ng/mL (95％ CI 71.4-95.4 ng/mL),which was 63 times higher than that from PP containers (GM 1.3 ng/mL;95％ CI 1.3-1.5 ng/mL).Concentrations of urinary 4-n-NP and creatinine were similar between two collection methods.Confirmation experiment showed that urinary BPA concentration collected by urine catheter and collection bags was much higher than those collected by other three methods.Conclusion· In collection of urine samples for measuring phenolic chemicals,PP urine collection container as well as LDPE containers are adequate for use in epidemiologic studies,but urine catheter and collection bag is not.

AIM: To investigate the ameliorative effect of water extract of propolis (WEP) preconditioning on mesenteric microcirculation of rats subjected to small intestinal ischemia reperfusion (I/R). METHODS: Thirty-two male Wistar rats were randomly divided into sham, I/R and WEP (100, 200 mg/kg) preconditioning groups. Model of small intestinal I/R injury was made by clamping super mesenteric artery for 45 min followed by 120 min of reperfusion in rats. Mesenteric microcirculation was detected at the end of reperfusion. The degree of small intestinal injury was The content of soluble intercellular adhesion molecule-1 (sICAM-1) in plasma and myeloperoxidase (MPO) activity in intestinal tissue were detected using enzyme linked-immuno-sorbent assay (ELISA) and spectrophotometer, respectively. RESULTS: (1) WEP preconditioning alleviated significantly the pathologic lesion in small intestine, and wet/dry ratio, compared to those in I/R group (P<0.01). (2) The disturbance of the blood flow in microvessel induced by I/R was improved significantly by WEP. In addition, WEP preconditioning alleviated significantly the decrease in diameters of microvessels and microcirculatory blood velocity (P<0.05 vs I/R group) and inhibited the adherence of leukocytes to venule (P<0.01 vs I/R group) in a dose-dependent manner. SICAM-1 content in plasma and MPO activity in intestinal tissue were decreased in WEP preconditioning group, compared to those in I/R group (P<0.05 or P<0.01). CONCLUSION: WEP preconditioning ameliorates mesenteric microcirculation of rats subjected to small intestinal I/R through suppressing the activation of polymorphonuclear neutrophils mediated by ICAM-1.

Objective To explore the relationship between the polymorphisms of the primary gout predisposing gene (BCAS3) rs11653176 locus and the incidence of gout in Han Chinese men in coastal areas of Shandong Province. Methods One hundred and fifty-two cases of patients with gout remission,68 cases of acute stage,252 patients with hyperuricemia, and 280 healthy subjects, total males, were enrolled. Genotyping the rs11653176 locus of BCAS3 gene by TaqMan probe technique. The expression level of BCAS3 gene mRNA in each PBMC was measured by RT-qPCR. The levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-18(IL-18)in serum were measured by ELISA. Results The change of allele frequency of rs11653176 locus in BCAS3 gene was associated with gout(P<0.01). BCAS3 mRNA in patients with gout was significantly higher than that of healthy people and patients with hyperuricemia(P<0.01). In gout patients, the expression level of BCAS3 gene containing C allele was higher than that of T allele(mRNA,P<0.05). The inflammatory factors in the acute phase of gout were significantly higher than those in phases of remission and hyperuricemia(P<0. 01). Conclusion Changes in the allele frequency of BCAS3 alleles rs11653176(high C, low T)may contribute to the expression of this gene,and lead to gout. And the onset of gout is closely related to the production of inflammatory factors.