Aim To investigate the expression of G protein-coupled receptor TGR5 and its effects on FN and TGF-β1 expression cultured under high glucose condition in rat glomerular mesangial cells , and then to explore the role of TGR5 in diabetic nephropathy. Methods INT-777 and TGR5 plasmid were used to activate TGR5 under high glucose(HG,30 mmol·L - 1 glucose ) condition, and anti-TGR5 small interfering RNA(TGR5 siRNA) was used to knock down TGR5. The protein expression of FN and TGF-β1 in rat me-sangial cells was detected by Western blot. Results TGR5 could be detected in rat glomerular mesangial cells. Both FN and TGF-β1 protein levels could be in-creased by high glucose compared with control group(P < 0. 05),and be inhibited by activiation of TGR5(P <0. 05). On the other hand,knockdown of TGR5 could increase FN and TGF-β1 protein to abnormal levels(P< 0. 01,P < 0. 05). Conclusion TGR5 suppresses HG-induced FN and TGF-β1 expression in rat glomer-ular mesangial cells,suggesting a protective role in the process of diabetic nephropathy.

Objective:To explore CPEB4 expression in patients with NSCLC and its prognostic value.Methods:The CPEB4 mRNA expres-sion levels were detected by real-time quantitative PCR,while the expression levels of proteins were detected by Western blot.The protein expression in formalin-fixed samples were analyzed by immunohistochemistry.The prognostic significance of CPEB4 in NSCLC patients was investigated by Log-rank and Cox proportional hazard regression model.Results:The mRNA and protein expression levels of CPEB4 in NSCLC cell lines were both higher than those in normal lung cell line.The mRNA and protein expression levels of CPEB4 were significantly upregulated in NSCLC tissues compared to the adjacent non-tumor tissues.The CPEB4-positive expression ratio was 38.2% in the formalin-fixed samples.N stages were identified as the relative factors of CPEB4 expression(P<0.05).Cox multivariate analysis showed significant relationships between overall survival and T stage,and between N stage and CPEB4 expression.Conclu-sions:CPEB4 expression was elevated in NSCLC and was related with the prognosis.Hence,it may be a potential target in NSCLC treat-ment.