Objective:To clone human CGI-100 gene and reconstruct its eukaryotic expressive vector for further investigation.Methods:The full coding domain sequence of human CGI-100 gene was cloned from human leukemic K562 cells with RT-PCR and was sub-cloned into pMD18-T Simple vector.After confirmed by DNA sequencing,the targeted DNA fragment,digested with BamHⅠand PstⅠ,was directionally cloned into eukaryotic expressive plasmid pIRES2-EGFP,then,the reconstructed plasmid was identified with enzyme digestion,PCR and sequencing.Results:It was demonstrated that the full coding domain sequence of human CGI-100 gene was accurately cloned into digestion sites between BamHⅠand PstⅠin pIRES2-EGFP without mutation and transposition.Conclusion:The reconstruction and verifying of eukaryotic expressive plasmid containing CGI-100 gene are successful,which establishes the foundation of further investigation.

Objective To observe the clinical effect of Guizhi decoction combined with reduced glutathione in the treatment of pulmonary heart failure patients with acute liver injury.Methods 72 patients with pulmonary heart failure and acute liver injury were divided into treatment group and control group.Patients in the control group were given conventional treatment for pulmonary heart disease,patients in the treatment group were treated with Guizhi decotion and reduced glutathione for three weeks.After three weeks,the clinical efficacy and clinical indicators were evaluated.Results In the treatment group,clinical symptoms,signs,liver function improvement,and oxidized antioxidant indicators,the difference were statistically different compared with those in the control group (t =10.25,5.45,3.23,17.34,11.49,11.61,all P ＜0.05).The total effective rate of the treatment group was 88.9％,which was significantly higher than thant of the control group 52.8％ (x2 =11.36,P ＜ 0.05).After treatment,the plasma lipid peroxide (LPO),superoxide dismutase(SOD) had statistically significant differences between the two groups (t =53.08,-9.03,all P ＜ 0.05).Conclusion Guizhi decoction combined with the reductive glutathione can improve symptoms of pulmonary heart failure patients with liver dysfunction,and have good clinical efficacy.

Objective Observe the synergy nerve protective effect and its mechanism of Sodium Ferulate (SF) and Ginseng saponins Rg1.Methods Ischemia-reperfusion injury models of rats were copied.The effects of SF,Ginsengsaponins Rg1 and the combination on infarction volume,lectin markers positive cells,peroxidase proliferation of activated receptorγ(PPAR-γ) mRNA expression,SOD activity and MDA content were observed.Results The blank control group didn't show infarction areas under TTC staining,and the cerebral infarction volume percentage of model control group,sodium ferulate group,ginsenoside Rg1 group and combination group was 44.2％,25.0％,20.4％,6.2％ respectively.The lectin positive cells number of blank control group,model control group,sodium ferulate group,ginsenoside Rg1 group and combination group were 11.4,44.6,27.8,23.0,13.4/500μm2 respectively; the PPAR-γmRNA expression were1883,1022,1473,1537,1843 respectively; the MDA content was 1.52,3.50,2.62,2.38,1.66 mmol/mg respectively; and the SOD acitivity was 71.54、73.14、81.72、82.22、91.10 U/mg respectively.Compared with model control group,sodium ferulate group,ginsenoside Rg1 group and combination group reduced the volume of cerebral infarcts (P＜0.01),inhibited the microglia activation(P＜0.01),increased the p PPAR-γ mRNA expression(P＜0.01),decreased the MDA content(P＜0.01) and increased SOD activity(P＜0.05,P＜0.01)significantly,and the effect of combination group was better than either sodium ferulate group or ginsenoside Rg1 group(P＜0.05,P＜0.01).Conclusion SF and Ginseng saponins Rg1 had collaborative neural protection and its activation on PPAR-γ may be one of the mechanisms.

Pruritus is one of the common clinical manifestations of cholestatic liver diseases such as primary biliary cirrhosis,primary scle-rosing cholangitis,and intrahepatic cholestasis of pregnancy.Potential incentives of itching include bile salts,histamine,progesterone me-tabolites,endogenous opioid substances,and lysophosphatidic acid,according to previous studies.However,the exact pathogenesis of chol-estatic pruritus remains unclear,and the current treatment can only alleviate the symptoms in some of the patients.Novel methods for treating pruritus have been proposed and/or are being studied.The recent experimental and clinical studies on the pathogenesis and treatment of pru-ritus in cholestatic hepatitis are reviewed,in order to improve the understanding and management of cholestatic pruritus.

Objective:To compare the level of low density lipoprotein cholesterol (LDL-C)before and after treat-ment between Uygur and Kazak nationality patients diagnosed as coronary heart disease (CHD).Methods:A total of 191 Uygur nationality patients and 184 Kazak nationality patients,who received coronary angiography and were diagnosed as CHD first time, and received measurement of LDL-C concentration.They received CHD secondary preventive treatment for 8~12 months,LDL-C concentration was measured again after treatment,and it's com-pared and analyzed between two nationalities.Results:After treatment,there were significant reductions in levels of total cholesterol (TC)and LDL-C in Uygur and Kazak nationality CHD patients.Compared with Kazak nationality pa-tients,there were significant reductions in levels of LDL-C [(2.74±0.13)mmol/L vs.(2.21±0.17)mmol/L]in Uygur nationality CHD patients (t=3.141,P<0.01).Conclusion:Effect lowering in Uygur nationality patients with coronary heart disease ,especially low density lipoprotein cholesterol is better than that of Kazak nationality.

Objective To study the best process of puerarin refined.Methods Results of the research on the selection of dissolved agent, agent and precipitator, and column chromatography of the resin, alumina, silica gel, fly ash and activated carbon were used to study the combined process. Results The average purity of optimization of the three craft products were 99.06052%, 99.22620% and 99.69880%.The yield of average values were 44.180%, 44.438% and 42.642%.Conclusion This study optimized the optimum process is feasible, puerarin purity is more than 99%, the yield is above 40%.

By clinical observing and summarizing a large amount of data, we found that the regularity of tongue presentation in stroke patient has important clinical significance. In the acute stage, the tongue coating and body of tongue should be observed carefully to tell the seriousness and the tendency of a disease; in the stable stage, the tongue coating and the texture of tongue should be observed carefully to tell progress of diseases; and in the recovery stage the texture of tongue should be observed carefully to judge the changes of Qi and Xue in viscera and prevent the relapse prevention of disease.

Objective This research summarized the nursing experience of critically ill patients infected by H7N9 avian influenza who were treated with extracorporeal membrane oxygenation (ECMO) and artificial liver device.Methods 10 critically ill patients infected by H7N9 avian influenza were selected,during treatment of ECMO combined with artificial liver device,nursing care such as careful observation of the changes of symptoms,strict disinfection and protection,contact isolation,symptomatic treatment to fever,correct management of airway and ventilator circle,pipeline nursing of ECMO and artificial liver device,mental nursing,symptomatic and support therapy which included antivirus,anti-hypoxia and anti multiple organ failure,anti-shock,anti-infection,microecological balance maintenance and water-electrolyte balance maintenance.Results 4 critically ill patients infected by H7N9 avian influenza improved and removed ECMO and artificial liver device,among whom one patient rehabilitated and was discharged.Another 6 patients were in a steady state.Conclusions For critically ill patients infected by H7N9 avian influenza,comprehensive and elaborate care can facilitate early recovery of patients.

Objective To disucss the coordination strategy between nurses and pharmacists in phar-macy intravenous admixture services (PIVAS) during the handling of clinical work. Methods Ques-tionnaires were adopted to investigated relative staff for information collection and analysis by combina-tion of specified post responsibility of nurses and pharmacists in PIVAS and experience from clinical work. Results We proposed the sixteen-word guideline of "clear duty, study and respect each other to achieve win- win development". Conclusions The coordination of nurses and pharmacists in PIVAS is important in clinical work, the countermeasures summarized from clinical practice has certain instructive significance to coordinate their relations.